FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964790
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45540.472019817784181No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36820.3816374547829061No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACGGCCTGGAGCCTGAAGATT34640.3590418640325874No Hit
GCGTAGCACCTGGCCTCTCACTTTCGGCGGAGGTACCAAGGTTAACATCAAACGAACTGTGGCTGCACCAT34620.35883456503487804No Hit
CTCCTGCACGGCCAGTCAGAGTATTAGCGACTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCCGGCT30240.31343608453653127No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29310.30379668114304664No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28470.29509012323925415No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27880.2889748028068284No Hit
GTGCTACGCTGCTGACAAAAATAAACCGCAGAATCTTCAGGCTCCAGGCCGTTGATGGTGAGAGTGAAGTCTGTC27630.2863835653354616No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27390.28389597736294947No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCAAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGAGTATTAGCGA26150.27104343950497No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT25480.2640989230817069No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24150.25031353973403536No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23910.24782595176152325No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23090.23932669285544003No Hit
GTGCAGCCACAGTTCGTTTGATGTTAACCTTGGTACCTCCGCCGAAAGTG22200.23010188745737412No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21650.22440116502036714No Hit
GACTTCACTCTCACCATCAACGGCCTGGAGCCTGAAGATTCTGCGGTTTATTTTTGTCAGCAGCGTAGCACCTGG21110.21880409208221477No Hit
GAGTATTAGCGACTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGATACAT21070.2183894940867961No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21020.21787124659252274No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19910.206366152219654No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19590.20304936825630446No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18510.19185522237999977No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17980.18636179894070212No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT17760.1840815099658993No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17390.1802464785082764No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17390.1802464785082764No Hit
ATGTTAACCTTGGTACCTCCGCCGAAAGTGAGAGGCCAGGTGCTACGCTG16610.17216181759761193No Hit
GGTTAACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC16600.17205816809875724No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16550.1715399206044839No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16480.17081437411250117No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTTAACCTTGGTAC16040.16625379616289554No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15540.1610713212201619No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA15370.15930927973963246No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15140.15692534126597496No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14810.15350490780377077No Hit
CTCCAGGCACCCTGTCTTTGTCTCAAGGGGAAAGAGCCACCCTCTCCTGC14690.1522611138175147No Hit
GTACCAAGGTTAACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14630.15163921682438666No Hit
GTCTTTGTCTCAAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGAGTATTAGCGACTTCTTAGCCTGGTA14510.15039542283813057No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14090.14604214388623432No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13960.14469470040112356No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13620.14117061744006468No Hit
GTGCAGGAGAGGGTGGCTCTTTCCCCTTGAGACAAAGACAGGGTGCCTGG13560.14054872044693664No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13120.13598814249733104No Hit
CCTTGGTACCTCCGCCGAAAGTGAGAGGCCAGGTGCTACGCTGCTGACAAAAATAAACCGCAGAATCTTCAGGCT13000.13474434851107495No Hit
ACCCTCTCCTGCACGGCCAGTCAGAGTATTAGCGACTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCC12770.13236041003741747No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12380.12831807958208521No Hit
CTGCTACTCTGGCTCCCAGATACCACCGCAGAAATTGTCTTGACACAGTC12360.1281107805843759No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12320.12769618258895718No Hit
GTCTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCAAGGGGAAAGAGC12160.12603779060728243No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12090.1253122441152997No Hit
CACCATCAACGGCCTGGAGCCTGAAGATTCTGCGGTTTATTTTTGTCAGCAGCGTAGCACCTGGCCTCTCACTTT11830.1226173571450782No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11710.12137356315882213No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11180.11588013971952445No Hit
ACATCTAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTC11090.1149472942298324No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAACGGCC10990.11391079924128567No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10650.11038671628022678No Hit
CCCAGATACCACCGCAGAAATTGTCTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCAAGGGGAAAGAGCCAC10600.10986846878595342No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10590.10976481928709875No Hit
GTATTAGCGACTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCC10410.10789912830771464No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10350.10727723131458658No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10230.10603343732833051No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10180.10551518983405715No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10150.10520424133749313No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG10030.10396044735123705No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9960.10323490085925435No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9950.10313125136039968No Hit
CATCTAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCT9890.10250935436727164No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9850.10209475637185295No Hit
GTCAGAGTATTAGCGACTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGATA9690.10043636439017818No Hit
ATCTAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAA9670.10022906539246884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA1050.045.23450529
GTCGTAC452.1597025E-645.0164341
TCGTACC452.1597025E-645.0164342
GGTATCA7250.044.705971
ACCGTAC1100.043.0930927
CGTATGC1050.042.8084441
TACTAGA1250.040.77477331
TCGTATG1100.040.76737640
CTAGATC1200.039.73750333
GTATCAA20550.038.28041
GATCTCG1200.037.0541336
ATCTCGT1250.035.6299737
TACACAT1650.034.7854275
CTTATAC1400.033.7623251
TAGATCT1450.032.92236334
TATCAAC23750.032.8383032
ACGAGAC1450.032.61670322
GCAGCGT18300.032.3224970
GTATGCC1400.032.17465242
TCTCCGA1600.031.64725911
Produced by FastQC (version 0.11.5)