FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964790
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44910.4654898993563366No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACGGCCTGGAGCCTGAAGATT34400.3565542760600753No Hit
GTGCTACGCTGCTGACAAAAATAAACCGCAGAATCTTCAGGCTCCAGGCCGTTGATGGTGAGAGTGAAGTCTGTC32100.33271489132350046No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31840.33002000435327894No Hit
GCGTAGCACCTGGCCTCTCACTTTCGGCGGAGGTACCAAGGTTAACATCAAACGAACTGTGGCTGCACCAT31790.3295017568590056No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29110.3017236911659532No Hit
CTCCTGCACGGCCAGTCAGAGTATTAGCGACTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCCGGCT28680.29726676271520225No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27750.2876273593217177No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26570.27539671845686625No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25990.26938504752329523No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24350.25238652971112885No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCAAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGAGTATTAGCGA24200.2508317872283088No Hit
GTGCAGCCACAGTTCGTTTGATGTTAACCTTGGTACCTCCGCCGAAAGTG23210.2405704868416961No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT23020.23860114636345733No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22410.23227852693332227No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20880.21642015360855732No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20660.21413986463375448No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20630.2138289161371905No Hit
GAGTATTAGCGACTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGATACAT20320.2106157816726956No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19790.20512235823339792No Hit
GACTTCACTCTCACCATCAACGGCCTGGAGCCTGAAGATTCTGCGGTTTATTTTTGTCAGCAGCGTAGCACCTGG19770.2049150592356886No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17880.1853253039521554No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17720.18366691197048063No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17720.18366691197048063No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17660.18304501497735257No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17240.17869173602545632No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17150.17775889053576427No Hit
ATGTTAACCTTGGTACCTCCGCCGAAAGTGAGAGGCCAGGTGCTACGCTG17050.17672239554721753No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT16970.17589319955638014No Hit
GGTTAACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC16220.16811948714227967No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16000.16583919816747686No Hit
GTCTTTGTCTCAAGGGGAAAGAGCCACCCTCTCCTGCACGGCCAGTCAGAGTATTAGCGACTTCTTAGCCTGGTA15780.16355890919267405No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15760.16335161019496472No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15550.16117497071901657No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTTAACCTTGGTAC15260.15816913525223106No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15250.15806548575337637No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA15240.1579618362545217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15110.15661439276941094No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14410.14935892784958385No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14190.14707863887478104No Hit
GTACCAAGGTTAACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13910.1441764529068502No Hit
CTCCAGGCACCCTGTCTTTGTCTCAAGGGGAAAGAGCCACCCTCTCCTGC13810.14313995791830347No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13750.14251806092517544No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13360.13847573046984318No Hit
GTGCAGGAGAGGGTGGCTCTTTCCCCTTGAGACAAAGACAGGGTGCCTGG13150.13629909099389506No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13090.135677194000767No Hit
CCTTGGTACCTCCGCCGAAAGTGAGAGGCCAGGTGCTACGCTGCTGACAAAAATAAACCGCAGAATCTTCAGGCT12850.13318960602825483No Hit
CTGCTACTCTGGCTCCCAGATACCACCGCAGAAATTGTCTTGACACAGTC12810.13277500803283615No Hit
GTCTTGACACAGTCTCCAGGCACCCTGTCTTTGTCTCAAGGGGAAAGAGC12660.13122026555001606No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12230.12676333709926513No Hit
ACCCTCTCCTGCACGGCCAGTCAGAGTATTAGCGACTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCC11940.1237575016324796No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11910.12344655313591558No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11240.11650203671265251No Hit
CACCATCAACGGCCTGGAGCCTGAAGATTCTGCGGTTTATTTTTGTCAGCAGCGTAGCACCTGGCCTCTCACTTT11170.11577649022066977No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11140.11546554172410575No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11030.11432539723670436No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11010.114118098238995No Hit
GTATTAGCGACTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCC10860.11256335575617492No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAACGGCC10600.10986846878595342No Hit
ACATCTAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTC10530.10914292229397071No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10510.10893562329626137No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10450.10831372630313334No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10380.1075881798111506No Hit
GTCAGAGTATTAGCGACTTCTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGATA9970.10333855035810902No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9840.10199110687299827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG307.005862E-445.03496616
GAATCGT405.6695902E-542.2180917
GGTATCA9900.038.8454481
GTATCAA23650.034.518721
ACGGGAT851.4551551E-731.78938716
AATGGTA5550.031.31945670
TATCAAC26200.031.1542282
TCAACGC27050.030.5493354
TTAACGG450.00486351530.34937535
CAACGCA27250.030.3251195
AACGCAG27250.030.2060386
TGGTACG1002.3127359E-730.15250667
AGAGTAC27750.029.2012611
CGCAGAG28500.028.539438
ACGCAGA28850.028.533797
GAGTTTA3050.027.6509861
GGTCTAT5600.027.458370
GTGGTGA5850.027.4276970
TCGCTAT1005.979946E-727.05046324
AAAACCG1507.632871E-526.74199770
Produced by FastQC (version 0.11.5)