FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207613
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGAACATTGTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA52730.4366465084426882No Hit
GACTTAGGACGGTCAGTCTGGTCCCTCCGCCGAACACCCAGGCACTGCGG47480.3931723159654624No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC46190.3824900858139156No Hit
GTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG40950.33909870132236075No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC40870.33843623743699347No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG39250.32502134375830666No Hit
CCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39110.323862031958914No Hit
CCCAGGCACTGCGGCTGAGATCCCATGTTCCGCAGTAGTAATTGGCCTCG37870.3135938417357216No Hit
GCCTTGGGCTGACTTAGGACGGTCAGTCTGGTCCCTCCGCCGAACACCCAGGCACTGCGGCTGAGATCCCATGTT36420.3015866838134402No Hit
GGGTTTATGGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34970.2895795258911588No Hit
GTCTGGTCCCTCCGCCGAACACCCAGGCACTGCGGCTGAGATCCCATGTTCCGCAGTAGTAATTGGCCTCGTCAG34010.28162995926675183No Hit
GTAGTAATTGGCCTCGTCAGCAGTCTGGAGTCCGGCGATGAACAGGGTGG32580.26978841731581227No Hit
GGACCAGACTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG31820.2634950104048234No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGG31650.26208727464841797No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTCTGGTCCCTC31490.2607623468776835No Hit
GTCCTGGGCCCAGCCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCGGGACAGCAGGTCACCATCTC31400.26001707500664534No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGT31030.25695317953682184No Hit
GTGCCTGGGTGTTCGGCGGAGGGACCAGACTGACCGTCCTAAGTCAGCCC30300.25090819658284563No Hit
GGTTTATGGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29540.2446147896718568No Hit
CCACCACACCCTCCAAACAAAGTGACAACAAGTACGCGGCCAGCAGTTAC28620.23699645499013341No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC28090.23260763174957538No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAA27870.23078585606481544No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT27020.22374717728278845No Hit
CCCTCAGTGTCTGCGGCCCCGGGACAGCAGGTCACCATCTCCTGTTCTGG26240.21728815440045776No Hit
GGTAACTGCTGGCCGCGTACTTGTTGTCACTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG25730.21306494713124155No Hit
CACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25250.20909016381903803No Hit
GCTCAGGCGTCAGGCTCAGGTAACTGCTGGCCGCGTACTTGTTGTCACTTTGTTTGGAGGGTGTGGTGGTCTCCA24100.19956724546688387No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23990.1986563576245039No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23960.19840793366749115No Hit
GTTTGGGGGCTGTTCCTGGGAACTGTTGATACCAAGAAACATCCTCGAAG23750.1966689659684021No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAACTGCTGG23650.1958408861116931No Hit
GTTCATCGCCGGACTCCAGACTGCTGACGAGGCCAATTACTACTGCGGAA23120.19145206287113503No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAACTG22600.18714604761624792No Hit
GAACAGGGTGGCTGACGTGGCAGACCTGGAGCCAGTGAATCGGTCAGGAA21680.17952771293452455No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGA21290.1762982014933592No Hit
GTCTGGAGTCCGGCGATGAACAGGGTGGCTGACGTGGCAGACCTGGAGCC20990.17381396192323204No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20940.1733999219948775No Hit
CTCCTGTTCTGGAAGCAACTTCAACATTACCTTCGAGGATGTTTCTTGGTATCAACAGTTCCCAGGAACAGCCCC20860.17273745810951024No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20620.17075006645340848No Hit
ACCCTGTTCATCGCCGGACTCCAGACTGCTGACGAGGCCAATTACTACTG20600.1705844504820667No Hit
GCCCCCAAACTCCTCATTTATGAAAATCATAAGCGACCCTCACGAATTCCTGACCGATTCACTGGCTCCAGGT20570.17033602652505397No Hit
AATTACTACTGCGGAACATGGGATCTCAGCCGCAGTGCCTGGGTGTTCGG20480.16959075465401582No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTCTGGTCCCTCCGCCGAACAC20440.16925952271133218No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20410.16901109875431947No Hit
GTATCAACAGTTCCCAGGAACAGCCCCCAAACTCCTCATTTATGAAAATCATAAGCGACCCTCACGAATTCCTGA20350.16851425084029403No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG19850.16437385155674872No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCTCTGCACAGAGT19260.15948818040216528No Hit
GCTCTAGTCTCCCGTGGTGGGGAGTGAGGGATGACAACCTATGAACATTG18380.15220107766312552No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17670.14632171068049118No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17640.14607328672347847No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAACTGCTGGCCGCGTACTT17610.14582486276646575No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGAGTGAGGGATGACAACCTATGAACATTGTGTAGGGGC17490.14483116693841486No Hit
GACCTGGAGCCAGTGAATCGGTCAGGAATTCGTGAGGGTCGCTTATGATTTTCATAAATGAGGAGTTTGGGGG16730.13853776002742602No Hit
CTCCAGGTCTGCCACGTCAGCCACCCTGTTCATCGCCGGACTCCAGACTG16390.13572228851461518No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC16230.1343973607438807No Hit
ACTCCAGACTGCTGACGAGGCCAATTACTACTGCGGAACATGGGATCTCA16220.13431455275820978No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGAAAATCATAAGCGACCCTCACGAATTCCTGACCGATTCACTGG16020.13265839304479168No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCTCTGCACAGAGTCCTGGGCCCAGC15910.1317475052024117No Hit
GTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15630.12942888160362634No Hit
GCCCCGGGACAGCAGGTCACCATCTCCTGTTCTGGAAGCAACTTCAACATTACCTTCGAGGATGTTTCTTGGTAT15590.1290976496609427No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATGGCAGCC15260.1263649861338028No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCCTGAAG15200.12586813821977735No Hit
CTGTTGATACCAAGAAACATCCTCGAAGGTAATGTTGAAGTTGCTTCCAGAACAGGAGATGGTGACCTGCTGTCC15160.12553690627709374No Hit
GCGATGAACAGGGTGGCTGACGTGGCAGACCTGGAGCCAGTGAATCGGTC15040.12454321044904286No Hit
CTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTT14940.1237151305923338No Hit
GTTCCTGGGAACTGTTGATACCAAGAAACATCCTCGAAGGTAATGTTGAA14940.1237151305923338No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCTCTG14770.1223073948359284No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTCTGGTC14690.12164493095056116No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14590.12081685109385208No Hit
GGGTGGCTGACGTGGCAGACCTGGAGCCAGTGAATCGGTCAGGAATTCGT14480.11990596325147211No Hit
CGTCAGCAGTCTGGAGTCCGGCGATGAACAGGGTGGCTGACGTGGCAGAC14390.11916069138043398No Hit
GCAGTTACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTAC14240.11791857159537038No Hit
CAATAATATATCGCCGTGTCCTCACTTCTCAGGCTGCTCATTTGCAGATA14140.1170904917386613No Hit
GGACAGCAGGTCACCATCTCCTGTTCTGGAAGCAACTTCAACATTACCTT14080.11659364382463587No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGA14040.11626241188195224No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13910.11518590806823047No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCC13730.11369536432615415No Hit
CTTAAGAGGTGTCCAGTGTCAGATGCAGCTGGTGGAGTCTGGTGGAGGTG13680.11328132439779963No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA13620.1127844764837742No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG13480.1116251646843815No Hit
AGACACACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC13430.11121112475602696No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGTGACAACAAGTACGCGGCCAGCAGTTACCTGAGCCTGACGC13420.11112831677035608No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13210.10938934907126704No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13100.10847846122888707No Hit
GCCATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13020.1078159973435198No Hit
CAGCCACCCTGTTCATCGCCGGACTCCAGACTGCTGACGAGGCCAATTAC12850.1064082615871144No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA12840.1063254536014435No Hit
CCCCTACACAATGTTCATAGGTTGTCATCCCTCACTCCCCACCACGGGAG12830.1062426456157726No Hit
CTCCAGACTGCTGACGAGGCCAATTACTACTGCGGAACATGGGATCTCAG12810.10607702964443079No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12490.10342717410296179No Hit
TATTTATTGAGGGTTTATGGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12340.10218505431789819No Hit
GAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCC12340.10218505431789819No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT12280.10168820640387277No Hit
GCCTGGGTGTTCGGCGGAGGGACCAGACTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC12210.10110855050417641No Hit
GTTCATAGGTTGTCATCCCTCACTCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCT12180.10086012654716371No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT12100.10019766266179644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2350.043.4553031
GACGCGT1850.038.68484526
TCGTATG1900.038.1604840
AATGGTA7900.037.93831670
ACGCGTA1900.037.6855727
CGTATGC1950.037.23048841
GCGTAGT2000.035.8548429
CTTATAC2000.035.7419851
ATCAACG32900.034.764243
TCAACGC32950.034.7114874
AGACGCG2100.034.06255725
TTATACA2100.034.039992
CAACGCA33650.033.9894075
CTTGAAA2300.033.9476457
AACGCAG33750.033.8915026
TCTCGTA2150.033.6201238
AATCTCG2150.033.5372636
CGAGACG2150.033.2524923
ATCTCGT2200.032.8134337
ACGCAGA35600.032.1236347
Produced by FastQC (version 0.11.5)