FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207613
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGCCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCGGGACAGCAGGTCACCATCTC59130.4896436192720681No Hit
CCTATGAACATTGTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA47290.39159896423771523No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC46220.38273850977092827No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG41480.34348752456291876No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC40360.33421303016777726No Hit
GGGTTTATGGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG40140.3323912544830173No Hit
GTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG38410.3180654729619506No Hit
CCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36010.2981915564009331No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCGGGACAGCAGGTCAC34520.28585316653596804No Hit
GACTTAGGACGGTCAGTCTGGTCCCTCCGCCGAACACCCAGGCACTGCGG34100.28237523113779No Hit
GCCTTGGGCTGACTTAGGACGGTCAGTCTGGTCCCTCCGCCGAACACCCAGGCACTGCGGCTGAGATCCCATGTT32350.26788383364538143No Hit
GTGCCTGGGTGTTCGGCGGAGGGACCAGACTGACCGTCCTAAGTCAGCCC32290.267386985731356No Hit
GTCTGGTCCCTCCGCCGAACACCCAGGCACTGCGGCTGAGATCCCATGTTCCGCAGTAGTAATTGGCCTCGTCAG30810.2551314038520619No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGT30680.2540549000383401No Hit
GGTTTATGGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30540.2528955882389474No Hit
CCCAGGCACTGCGGCTGAGATCCCATGTTCCGCAGTAGTAATTGGCCTCG29370.24320705391545136No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGG29250.24221335808740052No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAA28650.23724487894714613No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT28520.23616837513342437No Hit
GGACCAGACTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG28250.23393255952030992No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC27480.22755634462365013No Hit
GTAGTAATTGGCCTCGTCAGCAGTCTGGAGTCCGGCGATGAACAGGGTGG26260.21745377037179955No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTCTGGTCCCTC25560.21165721137483615No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24500.20287956489372008No Hit
CCACCACACCCTCCAAACAAAGTGACAACAAGTACGCGGCCAGCAGTTAC24340.2015546371229856No Hit
CCCTCAGTGTCTGCGGCCCCGGGACAGCAGGTCACCATCTCCTGTTCTGG23880.19774546978212393No Hit
GGTAACTGCTGGCCGCGTACTTGTTGTCACTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG23780.19691738992541485No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAACTGCTGG23100.19128644689979324No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22790.18871939934399512No Hit
GCTCAGGCGTCAGGCTCAGGTAACTGCTGGCCGCGTACTTGTTGTCACTTTGTTTGGAGGGTGTGGTGGTCTCCA22750.1883881674013115No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTCTGGTCCCTCCGCCGAACAC22590.18706323963057703No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22560.18681481567356428No Hit
CTCCTGTTCTGGAAGCAACTTCAACATTACCTTCGAGGATGTTTCTTGGTATCAACAGTTCCCAGGAACAGCCCC22410.1855726958885007No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22350.18507584797447527No Hit
GTTCATCGCCGGACTCCAGACTGCTGACGAGGCCAATTACTACTGCGGAA22160.18350249624672804No Hit
GTATCAACAGTTCCCAGGAACAGCCCCCAAACTCCTCATTTATGAAAATCATAAGCGACCCTCACGAATTCCTGA22130.18325407228971533No Hit
CACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21500.17803716919244822No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAACTG21320.17654662545037195No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20780.172074994224143No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20070.16619562724150866No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCTCTGCACAGAGT19990.1655331633561414No Hit
CTCCAGGTCTGCCACGTCAGCCACCCTGTTCATCGCCGGACTCCAGACTG19770.16371138767138146No Hit
GCCCCCAAACTCCTCATTTATGAAAATCATAAGCGACCCTCACGAATTCCTGACCGATTCACTGGCTCCAGGT19670.16288330781467242No Hit
GTTTGGGGGCTGTTCCTGGGAACTGTTGATACCAAGAAACATCCTCGAAG19600.16230365191497606No Hit
ACCCTGTTCATCGCCGGACTCCAGACTGCTGACGAGGCCAATTACTACTG19500.16147557205826701No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG19450.16106153212991248No Hit
GTCTGGAGTCCGGCGATGAACAGGGTGGCTGACGTGGCAGACCTGGAGCC18770.15543058910429086No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18530.15344319744818913No Hit
AATTACTACTGCGGAACATGGGATCTCAGCCGCAGTGCCTGGGTGTTCGG18500.15319477349117638No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGA18440.15269792557715095No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18100.14988245406434014No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCTCTG18050.1494684141359856No Hit
GCTCTAGTCTCCCGTGGTGGGGAGTGAGGGATGACAACCTATGAACATTG17420.14425151103871853No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAACTGCTGGCCGCGTACTT17310.14334062319633856No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTC17270.14300939125365494No Hit
GGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCTCTGCACAGAGTCCTGGGCCCAGC17040.14110480758322408No Hit
GAACAGGGTGGCTGACGTGGCAGACCTGGAGCCAGTGAATCGGTCAGGAA16590.1373784482280333No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGAGTGAGGGATGACAACCTATGAACATTGTGTAGGGGC16360.1354738645576025No Hit
GGGTGGCTGACGTGGCAGACCTGGAGCCAGTGAATCGGTCAGGAATTCGT16220.13431455275820978No Hit
CTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTT16200.13414893678686798No Hit
GCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGA15210.12595094620544825No Hit
GTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15150.12545409829142282No Hit
GCCCCGGGACAGCAGGTCACCATCTCCTGTTCTGGAAGCAACTTCAACATTACCTTCGAGGATGTTTCTTGGTAT14810.12263862677861202No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGAAAATCATAAGCGACCCTCACGAATTCCTGACCGATTCACTGG14770.1223073948359284No Hit
GGCCCAGCCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCGGGAC14610.12098246706519389No Hit
ACTCCAGACTGCTGACGAGGCCAATTACTACTGCGGAACATGGGATCTCA14550.12048561915116845No Hit
GCAGTTACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTAC14270.11816699555238308No Hit
TCTCTGCACAGAGTCCTGGGCCCAGCCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCGGGACAGCAGGT14030.11617960389628133No Hit
GAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCC14020.11609679591061044No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14010.11601398792493953No Hit
CAATAATATATCGCCGTGTCCTCACTTCTCAGGCTGCTCATTTGCAGATA13870.11485467612554684No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13710.11352974835481233No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATGGCAGCC13600.11261886051243236No Hit
CTGCTGACGAGGCCAATTACTACTGCGGAACATGGGATCTCAGCCGCAGT13560.11228762856974875No Hit
GACCTGGAGCCAGTGAATCGGTCAGGAATTCGTGAGGGTCGCTTATGATTTTCATAAATGAGGAGTTTGGGGG13550.11220482058407785No Hit
CCCCTCTCCTCCTCACCCTTCTCATTCTCTGCACAGAGTCCTGGGCCCAG13550.11220482058407785No Hit
CTCCAGACTGCTGACGAGGCCAATTACTACTGCGGAACATGGGATCTCAG13540.11212201259840693No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGTGACAACAAGTACGCGGCCAGCAGTTACCTGAGCCTGACGC13350.11054866087065972No Hit
GGACAGCAGGTCACCATCTCCTGTTCTGGAAGCAACTTCAACATTACCTT13250.10972058101395067No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13220.10947215705693794No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTCTGGTC13120.10864407720022888No Hit
GTTCATAGGTTGTCATCCCTCACTCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCT13020.1078159973435198No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCCTGAAG12990.1075675733865071No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA12930.10707072547248167No Hit
CCCCTACACAATGTTCATAGGTTGTCATCCCTCACTCCCCACCACGGGAG12830.1062426456157726No Hit
GCCTGGGTGTTCGGCGGAGGGACCAGACTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC12720.10533175777339263No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGAGTGAGGGATGACAAC12710.10524894978772173No Hit
CTGCTTCAGCTGTGAGCGCAGAAGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACC12610.10442086993101268No Hit
CTGTTGATACCAAGAAACATCCTCGAAGGTAATGTTGAAGTTGCTTCCAGAACAGGAGATGGTGACCTGCTGTCC12580.10417244597399995No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGTCTGGTCCCTCCGCCGAACACC12470.10326155813161998No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA12430.10293032618893636No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCC12400.10268190223192362No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12270.10160539841820185No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12080.10003204669045464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT556.6555676E-660.63955770
TTGACGA200.00781014650.9771910
GTGGTAT3150.044.204861
GTACGTA558.686959E-539.27005469
GGTACGT509.540394E-538.6507568
GCTTGAC558.236082E-637.049751
AATGGTA8800.036.38373670
ATTATAC701.1023494E-633.9566463
ATCAACG34250.033.807933
TCAACGC34850.033.4207464
CAACGCA35700.032.623665
AACGCAG36550.031.871576
TCTAATG550.004107284431.41604469
TATAAGA553.5748153E-430.8696782
ACGCAGA38200.030.5075537
CGCAGAG38650.030.1548568
TCTCGCG4000.030.0165870
CCCAATT500.005380149529.72956767
GTATAAG803.1214604E-629.7169881
AGAGTAC39300.029.57700211
Produced by FastQC (version 0.11.5)