FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400780
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG41220.29426462399520265No Hit
GTATTACTGTCAGCAATATGATGGGTCGCTGTATACTTTTGGCCAGGGGA39590.2826282499750139No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC34500.2462913519610503No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33740.2408658033381402No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30720.21930638644183956No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC29450.2102400091377661No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCACTCTGTAGATGGTGAGAGTGAAGTCTCTCCCAGACACACTCGCA29330.20938334356572766No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA28500.20345807335912847No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG26920.1921786433272891No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAAGTATTAC26740.19089364496923145No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC26100.18632476191835978No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG25860.1846114307742829No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA25700.183469210011565No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC25250.18025671411642086No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC24310.1735461671354531No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23990.1712617256100173No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCCTCGTCCTGCTACTCTGGCTCCCAGACATCA23980.1711903368123474No Hit
GTGTATTACTGTCAGCAATATGATGGGTCGCTGTATACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA23860.17033367124030896No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23840.17019089364496923No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23610.16854895129856223No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23220.16576478818943732No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG22500.1606247947572067No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT22420.16005368437584774No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT22170.158268964434101No Hit
GTCCTGCAAGACCAGTCACAATATTTACAGAACCTTTTTAGGGTGGTACC21840.1559131341109953No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21480.15334313739487998No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC21380.1526292494181813No Hit
GACCAGCACTGAGCACAGAGGCCTCACCATGGAGTTGGGGCTGACCTGGG21220.1514870286554634No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21220.1514870286554634No Hit
GTCCATATAGTATTTCTCACTTCCATCTTCCTTGATGTTGGCCACCCACT21070.15041619669041534No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20890.1491311983323577No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19890.14199231856537073No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC19590.13985065463527463No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19420.13863704507488683No Hit
CTATATGGACTCTGTGAAGGGCCGGTTCACCATCTCCAGAGACGGCGCCA19230.13728065791915933No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG19120.13649538114479076No Hit
GTAATACACTGCAGAATCTTCAGGCTCCACTCTGTAGATGGTGAGAGTGAAGTCTCTCCCAGACACACTCGCA19110.13642399234712088No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19020.13578149316809207No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18560.13249760847527806No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGACCAGCACTGAGC18370.13114122131955053No Hit
GTATACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG18290.1305701109381916No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18290.1305701109381916No Hit
ACCCTGTCCTGCAAGACCAGTCACAATATTTACAGAACCTTTTTAGGGTGGTACCAGAAGAAATCTGGCCAGCCT18290.1305701109381916No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTTAGTAATACTTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG18160.12964205656848327No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT17600.1256442838989706No Hit
GCACTGAGCACAGAGGCCTCACCATGGAGTTGGGGCTGACCTGGGTTTTC17480.12478761832693214No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17370.12400234155256357No Hit
GAGTTGGGGCTGACCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTCCACCTGAAAGAGT17340.12378817515955397No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG17280.12335984237353474No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16970.12114678964576879No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16710.11929068090635218No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC16600.1185054041319836No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16040.11450763146247092No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG15990.11415068747412158No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15950.11386513228344207No Hit
GTCTTTGTCTCCAGGGGAGCGAGCCACCCTGTCCTGCAAGACCAGTCACA15820.11293707791373378No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15570.11115235797198703No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA15560.11108096917431716No Hit
CCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG15470.11043846999528834No Hit
GGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACG15420.11008152600693899No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGACCAGCACTGAGCACAGAGGCCTCACCATGGAGTTGGGGCTGAC15420.11008152600693899No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG15250.10886791644655121No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC15070.10758291808849355No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15030.10729736289781407No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15000.10708319650480447No Hit
CTCCAAGGCCTTTCCACTTGGTGACCAGCACTGAGCACAGAGGCCTCACC14930.10658347492111538No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14870.10615514213509616No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14840.10594097574208655No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT14380.10265709104927256No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14360.1025143134539328No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA14280.10194320307257386No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14260.10180042547723411No Hit
GTCTGGGAGAGACTTCACTCTCACCATCTACAGAGTGGAGCCTGAAGATT14150.10101514870286554No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14130.10087237110752581No Hit
AAAGTATACAGCGACCCATCATATTGCTGACAGTAATACACTGCAGAATC14130.10087237110752581No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC14060.10037264952383672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG200.007648767451.2475437
CTTATAC3450.046.873851
GGTATCA5200.046.6484951
CGTATGC3550.044.6826641
TATACAC3700.044.6172523
TCGTATG3550.044.58979440
ATCTCGT3450.043.57278437
TACGTAT650.001021507241.71251770
TTATACA3900.041.465332
ATGCCGT3700.041.26506444
TCTACAT3700.041.259332
ACGAGAC3900.040.61161822
CCGTCTT3900.040.32332247
TACATCT3800.040.27922434
GTATGCC3850.039.49469442
TCTCCGA3950.039.234611
GTATCAA14900.039.1172521
CTTGAAA4350.038.91438357
GTGGTAT1650.038.7952081
CCCACGA4100.038.62504619
Produced by FastQC (version 0.11.5)