FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400780
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47960.3423806736246948No Hit
GTATTACTGTCAGCAATATGATGGGTCGCTGTATACTTTTGGCCAGGGGA35730.25507217407444427No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC34610.24707662873541883No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG33650.24022330415911136No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33590.23979497137309214No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC32350.23094276046202827No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCACTCTGTAGATGGTGAGAGTGAAGTCTCTCCCAGACACACTCGCA27880.1990319679035966No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAAGTATTAC27050.19310669769699737No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA26410.18853781464612573No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26100.18632476191835978No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG25900.18489698596496237No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG24890.17768671740030553No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24630.17583060866088893No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24580.17547366467253958No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCCTCGTCCTGCTACTCTGGCTCCCAGACATCA23500.16776367452419366No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG23220.16576478818943732No Hit
GTGTATTACTGTCAGCAATATGATGGGTCGCTGTATACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA23190.1655506217964277No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC22910.16355173546167134No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22740.16233812590128355No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22690.1619811819129342No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG22650.16169562672225474No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC22530.16083896115021631No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22130.15798340924342152No Hit
GTCCTGCAAGACCAGTCACAATATTTACAGAACCTTTTTAGGGTGGTACC22020.15719813246905295No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC20960.14963091991604677No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC20750.1481317551649795No Hit
GTCCATATAGTATTTCTCACTTCCATCTTCCTTGATGTTGGCCACCCACT20570.14684675680692186No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20000.1427775953397393No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG19790.14127843058867204No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTTAGTAATACTTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG19160.13678093633547025No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC18960.13535316038207285No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18800.13421093961935493No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18680.1333542740473165No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18640.133068718856637No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18510.1321406644869287No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18480.1319264980939191No Hit
ACCCTGTCCTGCAAGACCAGTCACAATATTTACAGAACCTTTTTAGGGTGGTACCAGAAGAAATCTGGCCAGCCT18460.13178372049857937No Hit
GACCAGCACTGAGCACAGAGGCCTCACCATGGAGTTGGGGCTGACCTGGG18190.12985622296149288No Hit
GTAATACACTGCAGAATCTTCAGGCTCCACTCTGTAGATGGTGAGAGTGAAGTCTCTCCCAGACACACTCGCA17950.128142891817416No Hit
CTATATGGACTCTGTGAAGGGCCGGTTCACCATCTCCAGAGACGGCGCCA17430.12443067433858279No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17120.12221762161081683No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT17020.12150373363411815No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16980.12121817844343864No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGACCAGCACTGAGC16690.11914790331101242No Hit
GTCTTTGTCTCCAGGGGAGCGAGCCACCCTGTCCTGCAAGACCAGTCACA16420.11722040577392596No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16190.11557846342751896No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA16120.11507874184382987No Hit
GTATACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG16090.11486457545082027No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16040.11450763146247092No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15670.11186624594868573No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15550.11100958037664731No Hit
GAGTTGGGGCTGACCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTCCACCTGAAAGAGT15380.10979597081625951No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15290.10915347163723069No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG15210.10858236125587174No Hit
GGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACG15000.10708319650480447No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14990.10701180770713459No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14980.10694041890946472No Hit
CACTTGCACTGCTGCCTACCCCGAGTCCAAGACCCCGCTAACCGCCACCC14820.10579819814674682No Hit
GCACTGAGCACAGAGGCCTCACCATGGAGTTGGGGCTGACCTGGGTTTTC14790.1055840317537372No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14420.10294264623995203No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14420.10294264623995203No Hit
GGGTTCCCTGGCCCCAGTTGTCAAAGAATGGACGAGCTGACATCCTTGTCGTACAATAATATATGGCCGTGTCTT14290.10201459187024371No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG14090.10058681591684634No Hit
GTGTGAGGTCCACCTGAAAGAGTCTGGGGGAGGAATTGTCCAGCCGGGGGGGTCCCTGAGACTCTCCTGTGCAGC14070.10044403832150658No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGACCAGCACTGAGCACAGAGGCCTCACCATGGAGTTGGGGCTGAC14040.10022987192849699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC2600.043.979252
GTGGTAT2850.041.3132671
TAGGAAC350.001528192538.4342774
GGTATCA6900.037.5411831
GTATCAA18000.035.322841
GTTATGC502.125172E-433.7311426
TAAGCCT400.002941848533.6323935
CATCTAA1052.2949644E-433.28941370
ATTAGAG951.1286829E-831.8599933
TATCAAC20250.031.2230342
ATCAACG20700.030.5431843
AAGTGCG450.005199786329.93821314
TCAACGC21250.029.7526534
CAACGCA21600.029.4283455
AACGCAG21550.029.3531326
TAACGGC704.152122E-529.2475136
ACGGGTG1501.8189894E-1229.18975816
AGAGTAC21850.028.51768311
CGCAGAG22350.028.1710328
TAAGTCA1755.0078725E-627.96310670
Produced by FastQC (version 0.11.5)