FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919897
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22240.2417661977373554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21960.23872237870109372No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21250.2310041232877159No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15530.16882324868979895No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13180.14327691034974568No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC13100.14240724776795663No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12510.1359934862272624No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACC12050.13099292638197535No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG12000.13044938726835723No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11070.12033955975505953No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC10090.10968619312814369No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA9310.10120698295570046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGT400.001480568238.6805863
GTATCAA47050.036.540761
GGTATCA19550.031.2753121
ATCAACG56100.030.4083443
TATCAAC56950.030.2430692
ACGGGTA4150.030.1248816
TCAACGC56750.030.0021384
CAACGCA57050.029.9628565
CGCAGAG57050.029.64138
AGAGTAC57400.029.35231811
AACGCAG58300.029.3220216
GCAGAGT57900.029.0372689
CAGAGTA58400.028.84813910
ACGCAGA60900.028.0268637
TACGGGT14650.027.39756615
GACCGGA2056.4013693E-726.83397170
GAGTACG43150.026.46262712
ATCGTCG500.00972442426.33395820
AGTACGG44300.025.77567713
ACGGGTC3450.024.79385616
Produced by FastQC (version 0.11.5)