FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697709
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAACTTGGTCCCCCCGCCGAACACCACAAAAGTGTAG30090.4312686234518976No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28710.41148960383197003No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27980.40102678910548667No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCCCCGCCGAACACCACAAAAGTGTAGCTGCCTGCATATGAG25850.37049830230081593No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGA20760.29754525167369206No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20190.2893756566132872No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19680.2820660189276618No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATT19170.27475638124203644No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19130.2741830763255168No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18950.27160320420117845No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG17490.2506775747482116No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCCC15990.22917864037872523No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15690.22487885350482795No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGTGGTTATAAATTTGTCTCCTGGTACCAACAGCACCCAGGCAAGGC15690.22487885350482795No Hit
ACTATAACCTGTCCCTCAAGAGTCGACTCACCATTTCCGTAGACACGTCC15280.21900247811050166No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14530.20825301092575843No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14470.207393053550979No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTACTCACTACTGGGGCTGGATCCG14050.20137335192752281No Hit
ACCTAGGACGGTCAACTTGGTCCCCCCGCCGAACACCACAAAAGTGTAGCTGCCTGCATATGAGCAGCAGAAATA14050.20137335192752281No Hit
GTGTATTACTGTGCGAGATCCTCCGGGTCGAGTATAGTAAATTGGTTCGA13920.19951011094883397No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13710.1965002601371059No Hit
TCTCTGGGCTCCTGGCTGAGGATGAGGCTGAGTATTTCTGCTGCTCATAT13220.1894772749097403No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG13190.18904729622235059No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG13010.1864674240980122No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12820.1837442257445439No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12800.18345757328628412No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12690.1818809847658551No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGTGGTTATAAATTTGTCTCCTGGTACCAACAGCA12400.17772452412108772No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12090.17328141101806055No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12040.172564779872411No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC11820.169411602831553No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11430.1638218798954865No Hit
GGATGAGGCTGAGTATTTCTGCTGCTCATATGCAGGCAGCTACACTTTTG11390.1632485749789669No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11220.1608120290837584No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11110.15923544056332942No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10590.15178247664857414No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10310.14776934223293667No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT10290.14748268977467682No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10250.14690938485815722No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC10150.1454761225668581No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC9940.14246627175513No Hit
GTATTTCTGCTGCTCATATGCAGGCAGCTACACTTTTGTGGTGTTCGGCG9800.14045970454731127No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT9730.1394564209434019No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9450.13544328652776444No Hit
GCCCTCAGGGGTCCCTGATCGCTACTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTC9380.13444000292385508No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGTAG9380.13444000292385508No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9370.13429667669472517No Hit
CCACAAAAGTGTAGCTGCCTGCATATGAGCAGCAGAAATACTCAGCCTCA9170.13143015211212697No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8970.12856362752952877No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8910.12770367015474934No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8690.12455049311389133No Hit
CCTCAGGGGTCCCTGATCGCTACTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCTGG8680.1244071668847614No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8640.12383386196824177No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT8590.12311723082259222No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG8500.12182729476042303No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8490.12168396853129314No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8480.12154064230216323No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT8470.1213973160730333No Hit
GCCTCATCCTCAGCCAGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC8440.1209673373856436No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8430.12082401115651367No Hit
GCAGAAATACTCAGCCTCATCCTCAGCCAGGAGCCCAGAGATGGTCAGGG8420.12068068492738376No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC8360.11982072755260431No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATGATGAGTTTGGGGGCCTTGCCTGGGTGCTGTTGGTACCAGGAGA8340.11953407509434448No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8190.11738418165739585No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA8140.1166675505117463No Hit
GAGTATTTCTGCTGCTCATATGCAGGCAGCTACACTTTTGTGGTGTTCGG8050.1153776144495771No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT8050.1153776144495771No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7980.11437433084566775No Hit
CTTTTGTGGTGTTCGGCGGGGGGACCAAGTTGACCGTCCTAGGTCAGCCC7930.11365769970001821No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7850.11251108986697893No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC7850.11251108986697893No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7820.1120811111795892No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7790.11165113249219946No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7780.11150780626306955No Hit
TCGCTACTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTG7750.11107782757567983No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7750.11107782757567983No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7740.11093450134654992No Hit
GTATTACTGTGCGAGATCCTCCGGGTCGAGTATAGTAAATTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCAC7720.11064784888829012No Hit
CTATTATAGTGGGAGCGCCTACTATAACCTGTCCCTCAAGAGTCGACTCACCATTTCCGTAGACACGTCCAGGGA7690.11021787020090038No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC7590.10878460790960129No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7580.10864128168047137No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7330.10505812595222362No Hit
GTACCAACAGCACCCAGGCAAGGCCCCCAAACTCATCATTTATGATGTCA7330.10505812595222362No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGA7250.10391151611918437No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7240.10376818989005444No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7230.10362486366092453No Hit
CTACTGGGGCTGGATCCGCCAGCCCCCGGGGAAGGGGCTGGAGTGGATTGCGACTATCTATTATAGTGGGAGCGC7160.10262158005701516No Hit
GCTCATATGCAGGCAGCTACACTTTTGTGGTGTTCGGCGGGGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGG7110.10190494891136562No Hit
GAGTAGCGATCAGGGACCCCTGAGGGCCGCTTAGTGACATCATAAATGATGAGTTTGGGGGCCTTGCCTGGGTG7030.10075833907832635No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7020.10061501284919644No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)