FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697709
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAACTTGGTCCCCCCGCCGAACACCACAAAAGTGTAG28010.40145676779287637No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25880.3709282809882057No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCCCCGCCGAACACCACAAAAGTGTAGCTGCCTGCATATGAG25570.3664851678851785No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25540.36605518919778873No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21010.3011284074019398No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19810.28392925990635065No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAACTTCAGGGAGA19000.27231983534682797No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG18480.2648668714320727No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18350.2630036304533839No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATT17270.24752439770735365No Hit
CTCCTGCACTGGAACCAGCAGTGATATTGGTGGTTATAAATTTGTCTCCTGGTACCAACAGCACCCAGGCAAGGC17090.24494452558301527No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16920.2425079796878068No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCCC15080.21613595352790343No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14470.207393053550979No Hit
TCTCTGGGCTCCTGGCTGAGGATGAGGCTGAGTATTTCTGCTGCTCATAT14330.20538648634316026No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14160.2029499404479518No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14080.20180333061491254No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTACTCACTACTGGGGCTGGATCCG13790.19764686997014516No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13660.19578362899145635No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13610.19506699784580678No Hit
ACCTAGGACGGTCAACTTGGTCCCCCCGCCGAACACCACAAAAGTGTAGCTGCCTGCATATGAGCAGCAGAAATA13400.19205714703407867No Hit
GTGTATTACTGTGCGAGATCCTCCGGGTCGAGTATAGTAAATTGGTTCGA13290.19048055851364967No Hit
ACTATAACCTGTCCCTCAAGAGTCGACTCACCATTTCCGTAGACACGTCC13070.18732738147279165No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG12830.18388755197367385No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATATTGGTGGTTATAAATTTGTCTCCTGGTACCAACAGCA12190.17471467330935964No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12160.1742846946219699No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG11950.1712748438102418No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11750.16840831922764363No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC11710.16783501431112396No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11700.16769168808199408No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11210.1606687028546285No Hit
GGATGAGGCTGAGTATTTCTGCTGCTCATATGCAGGCAGCTACACTTTTG10930.15665556843899106No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10720.15364571762726295No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10510.15063586681553484No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC10490.15034921435727502No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10260.14705271108728712No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9860.14131966192209072No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9790.14031637831818136No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9730.1394564209434019No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9560.13701987504819343No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9540.13673322258993365No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC9450.13544328652776444No Hit
GCCCTCAGGGGTCCCTGATCGCTACTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTC9390.134583329152985No Hit
CCACAAAAGTGTAGCTGCCTGCATATGAGCAGCAGAAATACTCAGCCTCA9320.13358004554907563No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT9220.1321467832577765No Hit
GTATTTCTGCTGCTCATATGCAGGCAGCTACACTTTTGTGGTGTTCGGCG9130.13085684719560733No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9100.1304268685082176No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT8860.1269870390090998No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8850.12684371277996986No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATGATGAGTTTGGGGGCCTTGCCTGGGTGCTGTTGGTACCAGGAGA8800.12612708163432032No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8760.12555377671780069No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8750.12541045048867078No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT8720.12498047180128104No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8610.12340388328085206No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGTAG8600.12326055705172212No Hit
GAGTATTTCTGCTGCTCATATGCAGGCAGCTACACTTTTGTGGTGTTCGG8420.12068068492738376No Hit
CTTTTGTGGTGTTCGGCGGGGGGACCAAGTTGACCGTCCTAGGTCAGCCC8330.11939074886521459No Hit
GCCTCATCCTCAGCCAGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCC8270.11853079149043513No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8180.11724085542826596No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8140.1166675505117463No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8100.11609424559522667No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8080.11580759313696684No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8020.11494763576218739No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7990.11451765707479766No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT7980.11437433084566775No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA7800.11179445872132938No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7790.11165113249219946No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7770.11136448003393966No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7670.10993121774264054No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC7630.10935791282612092No Hit
TCGCTACTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTG7580.10864128168047137No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT7460.10692136693091246No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7440.10663471447265263No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7330.10505812595222362No Hit
CCTCAGGGGTCCCTGATCGCTACTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCTGG7330.10505812595222362No Hit
CTATTATAGTGGGAGCGCCTACTATAACCTGTCCCTCAAGAGTCGACTCACCATTTCCGTAGACACGTCCAGGGA7310.10477147349396382No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG7270.10419816857744418No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7240.10376818989005444No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7210.10333821120266473No Hit
GCAGAAATACTCAGCCTCATCCTCAGCCAGGAGCCCAGAGATGGTCAGGG7150.10247825382788527No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7080.1014749702239759No Hit
GTACCAACAGCACCCAGGCAAGGCCCCCAAACTCATCATTTATGATGTCA7040.10090166530745627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAA5150.058.04865370
TAATACG200.007739260351.092634
GGTATCA6350.046.670631
CTACGGA5400.036.66631769
AATGGTA2004.474714E-1033.97165370
GTATCAA13950.033.4537241
GTGGTAT1550.032.9653471
TGGTATC1550.032.9629862
AGAAATA14300.031.35845270
ACGTGTC5700.030.76064763
GTGTCTA5900.029.22586365
TCTACGG6200.029.08589768
GTCTACG6050.029.07044667
GGACGTG6100.028.64218561
TATCAAC16300.028.628592
GTCGCTG2500.028.6139221
CGAGTAT5500.028.56171229
GGTCGAG5750.027.8977726
GACGTGT6300.027.78085562
TGTCTAC6250.027.74653466
Produced by FastQC (version 0.11.5)