FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974579
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC36940.3790354604398412No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAGCCCATTCAGGCTG35550.36477289167938154No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34200.3509207565523164No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAGCCCATTCAGGCTGTCATCCCATGCTGCA31860.32691038899873687No Hit
GTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCTGCGTCTGGGACCC31250.3206512760894704No Hit
ATCTTAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG28570.29315222265203744No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26420.2710914148570819No Hit
GTAATAATAACCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG26260.26944968032350375No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25930.26606360284799896No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25370.26031753198047564No Hit
CTATTAAGATAGATGAGGAGTCTGGGGGCCGCTCCGGGGAGGTGCTGGTA24220.2485175650203832No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCTGCGTCT24220.2485175650203832No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23680.24297671096955709No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22320.22902196743414335No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21460.22019764431616112No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21180.2173246088823995No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20740.21280983891505972No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20100.20624290078074736No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19740.20254899808019666No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAGCCCATTCAGGCTGT18550.19033859748670964No Hit
GTCTGGGGGCCGCTCCGGGGAGGTGCTGGTACCAGTTAACAGTATTACTT18530.1901333806700124No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18490.18972294703661785No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17860.18325861731065415No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC17190.17638385395129588No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17040.17484472782606642No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16940.17381864374258013No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16540.1697143074086349No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCAC16530.16961169900028628No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16340.1676621392416623No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16260.16684127197487325No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC15330.15729868999845062No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15150.15545173864817527No Hit
CCCCTGAGGGCCGCTGACTATTAAGATAGATGAGGAGTCTGGGGGCCGCTCCGGGGAGGTGCTGGTACCAGTTAA15040.15432304615634032No Hit
GCCCCCAGACTCCTCATCTATCTTAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT15000.1539126125229458No Hit
TAATAACCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAAT14950.15339957048120265No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14430.14806393324707387No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14420.14796132483872523No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14250.14621698189679852No Hit
ATCCCATGCTGCACAGTAATAATAACCCTCATCCTCAGACTGGAGCCCAC13960.14324133805468822No Hit
GTCTGAGGATGAGGGTTATTATTACTGTGCAGCATGGGATGACAGCCTGA13620.13975265217083477No Hit
GTGCTGATTCAGCCACCCTCTGCGTCTGGGACCCCCGGGCAGAGGGTCAC13570.13923961012909164No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13550.1390343933123944No Hit
GCATGGGATGACAGCCTGAATGGGCTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC13320.13667439992037586No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13260.1360587494702841No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13180.13523788220349506No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT12770.1310309374612012No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12690.13021007019441216No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC12480.12805529361909093No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12470.1279526852107423No Hit
GGGTTATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGGCTGTTCG12390.12713181794395323No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12270.12590051704376967No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12040.12354052365175117No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12020.12333530683505389No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA11850.12159096389312718No Hit
TATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGGCTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGT11690.11994922935954909No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11690.11994922935954909No Hit
ATGCTGCACAGTAATAATAACCCTCATCCTCAGACTGGAGCCCACTGATG11660.11964140413450321No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11600.11902575368441141No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG11510.11810227800927375No Hit
GTATTACTTGCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT11400.11697358551743882No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11330.1162553266589984No Hit
ATAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA11150.11440837530872305No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10730.11009882215808056No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10640.10917534648294289No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC10620.10897012966624563No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10550.10825187080780521No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10420.106917961499273No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10320.10589187741578672No Hit
GTGCTGGTACCAGTTAACAGTATTACTTGCGATGTTGGAGCTGCTTCCAG10260.10527622696569494No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10230.10496840174064904No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10190.10455796810725451No Hit
GTACCAGCACCTCCCCGGAGCGGCCCCCAGACTCCTCATCTATCTTAATAGTCAGCGGCCCTCAGGGGTCCCTGA9980.10240319153193328No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9970.10230058312358464No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGGTTATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGG9900.10158232426514423No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9870.10127449904009833No Hit
GTGCAGCATGGGATGACAGCCTGAATGGGCTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGG9850.10106928222340109No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAG9830.10086406540670383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCG306.7003164E-445.4445045
GGTATCA8850.045.0593761
GTATCAA22750.040.45061
ATACCGT350.001429929338.956436
GTGGTAT1700.038.0931851
TATCTCG601.3870451E-534.335336
TCTAGAC400.002755494834.0833742
TATCAAC27600.033.2189452
TCAACGC28650.032.1204574
ATCAACG28750.032.0087363
TCGTATG652.3107381E-531.86706740
AACGCAG29000.031.8535986
CAACGCA29200.031.6321745
ACGCCGG900.005457814329.63937270
TCTGGAG6300.029.63937270
CGTATGC703.7912127E-529.638641
TGGTATC2200.029.4356442
AGAGTAC31550.029.28206411
CAGAGTA31950.029.02216310
GCAGAGT32050.028.9301249
Produced by FastQC (version 0.11.5)