FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974579
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCTGCGTCTGGGACCC35250.36169463942892266No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34850.3575903030949774No Hit
ATCTTAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG32090.3292703823907554No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAGCCCATTCAGGCTG30180.30967217639616695No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30180.30967217639616695No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29090.2984878598861662No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAGCCCATTCAGGCTGTCATCCCATGCTGCA28060.2879191938262573No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCTGCGTCT27160.27868443707488055No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26400.2708861980403846No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25270.2592914478969894No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23610.2422584521111167No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22940.23538368875175844No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22420.23004805151762966No Hit
CTATTAAGATAGATGAGGAGTCTGGGGGCCGCTCCGGGGAGGTGCTGGTA21800.22368633020001458No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20190.20716637645588507No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20130.20655072600579324No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19670.20183073922175626No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18840.19331424132881994No Hit
GTCTGGGGGCCGCTCCGGGGAGGTGCTGGTACCAGTTAACAGTATTACTT18660.1914672899785446No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17500.17956471461010343No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCAC17390.1784360221182685No Hit
GTGCTGATTCAGCCACCCTCTGCGTCTGGGACCCCCGGGCAGAGGGTCAC17200.17648646235964452No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16990.1743316857843233No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16790.17227951761735066No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16690.17125343353386438No Hit
GCCCCCAGACTCCTCATCTATCTTAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT16580.17012474104202943No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC16140.1656099710746897No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16070.16489171221624926No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15450.15852999089863418No Hit
GTAATAATAACCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG15430.15832477408193693No Hit
GGGTTATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGGCTGTTCG14200.14570393985505536No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14170.14539611463000948No Hit
GTCTGAGGATGAGGGTTATTATTACTGTGCAGCATGGGATGACAGCCTGA14100.14467785577156905No Hit
CCCCTGAGGGCCGCTGACTATTAAGATAGATGAGGAGTCTGGGGGCCGCTCCGGGGAGGTGCTGGTACCAGTTAA13980.1434465548713855No Hit
GCATGGGATGACAGCCTGAATGGGCTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC13740.14098395307101835No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAGCCCATTCAGGCTGT13610.13965004376248616No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13600.13954743535413752No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13560.139137001720743No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13400.13749526718716493No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13320.13667439992037586No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13310.13657179151202725No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13230.13575092424523819No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13170.13513527379514642No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA13110.13451962334505463No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG12990.13328832244487107No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT12780.13113354586954984No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12530.12856833566083406No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12530.12856833566083406No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12340.12661877590221007No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT12230.12549008341037515No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12230.12549008341037515No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAG12180.12497704136863198No Hit
GTACCAGCACCTCCCCGGAGCGGCCCCCAGACTCCTCATCTATCTTAATAGTCAGCGGCCCTCAGGGGTCCCTGA11540.11841010323431964No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11400.11697358551743882No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11370.11666576029239292No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11340.11635793506734704No Hit
GTATTACTTGCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT11260.11553706780055797No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11050.11338229122523674No Hit
GTGCAGCATGGGATGACAGCCTGAATGGGCTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGG10990.11276664077514496No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10770.11050925579147508No Hit
GGCCCAGTCTGTGCTGATTCAGCCACCCTCTGCGTCTGGGACCCCCGGGC10620.10897012966624563No Hit
TATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGGCTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGT10570.10845708762450249No Hit
GTGCTGGTACCAGTTAACAGTATTACTTGCGATGTTGGAGCTGCTTCCAG10370.10640491945752986No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10330.10599448582413534No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC10320.10589187741578672No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10270.10537883537404355No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10140.10404492606551137No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10090.10353188402376821No Hit
ATCCCATGCTGCACAGTAATAATAACCCTCATCCTCAGACTGGAGCCCAC9920.1017875410818415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11450.041.020321
GTATCAA25200.036.4660451
GTGGTAT3000.036.3039741
CGACCAA553.0025654E-431.80099349
GGTAATC901.4526631E-731.79535161
CGAGCGA553.1023953E-431.62417245
TATCAAC28850.031.6068152
TGGTATC3600.030.2442
ATCAACG30500.029.7853833
TCAACGC30750.029.4325734
AACGCAG31000.029.2027076
CAACGCA31100.029.1043265
GTAATCA1003.579753E-728.6854462
GCTTGTA605.869592E-428.3624821
GCTTGGG1504.1836756E-1127.227981
AGAGTAC34500.026.16429311
GCAGAGT34800.025.7416529
CAGAGTA35000.025.69319310
TATACTG801.049872E-425.5209925
GCGACCA700.001240134724.9522748
Produced by FastQC (version 0.11.5)