FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045548
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGT47020.4497163210106088No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43140.4126065948191762No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42160.40323351964711324No Hit
CTCTATTACTGTGCGAAAACACGGGGTAACTACGACTTTGGTTGGGGGGCCTTTGACTCCTGGGGCCAGGGAAGC35170.3363786263280117No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG31110.2975473149008941No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31050.29697345315566576No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29460.2817661169071147No Hit
CTATGCCACTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGTTGTTGATGGTGAAAGTAAAATGTGTC27470.2627330356903748No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26770.25603798199604416No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26010.24876906655648523No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25540.2442738162188632No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24910.23824826789396567No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23980.22935341084292638No Hit
GTGGCATAGTGTCCGTCCAATGTTCACTTTCGGCCCTGGGACCAAGGTGG23220.22208449540336742No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAACACATTAACAACCAATTAAATTGGTATCAGCAGAAG22890.21892825580461156No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22430.2145286490911943No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22120.21156369674084785No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21940.20984211150516285No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21330.20400785042867473No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21170.20247755244139914No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20480.19587814237127327No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19920.19052209941580875No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19910.190426455791604No Hit
CTATTACTGTGCGAAAACACGGGGTAACTACGACTTTGGTTGGGGGGCCTTTGACTCCTGGGGCCAGGGAAGC19840.18975695042217094No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAACACATTAACAA19790.18927873230114733No Hit
ACATATTACTGTCAACAGTGGCATAGTGTCCGTCCAATGTTCACTTTCGGCCCTGGGACCAAGGTGGCCATCAAA19710.18851358330750956No Hit
GATCTACGATGCATCCAATTTGGAAGCAGGGGTCCCATCAAGGTTCAGCGGAAGTGGATCTGGGACACATTTTA19570.18717457256864342No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGTTGTTGATGGTGAAAGTAAAATGTGTCCCAGATCCACTTCCG19370.18526170008454898No Hit
ATATTACTGTCAACAGTGGCATAGTGTCCGTCCAATGTTCACTTTCGGCC18820.18000130075328918No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18800.17981001350487974No Hit
GTGCAGCCACAGTTCGTTTGATGGCCACCTTGGTCCCAGGGCCGAAAGTGAACATTGGACGGACACTATGCCA18700.1788535772628325No Hit
CAGTAATAGAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA18480.17674941753032858No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17690.16919357121815545No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGTTGTTGATGGTGAAAGTAAAATGTGTCCCAGATCCACTTCCG17600.16833277860031295No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17390.16632426249201376No Hit
GGCATAGTGTCCGTCCAATGTTCACTTTCGGCCCTGGGACCAAGGTGGCCATCAAACGAACTGTGGCTGCACCAT16900.1616377249059823No Hit
CCTTGGTCCCAGGGCCGAAAGTGAACATTGGACGGACACTATGCCACTGTTGACAGTAATATGTTGCAACATCTT16690.15962920879768314No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGGCCACCTTGGTCC16480.15762069268938395No Hit
GTATAGTAGGTGCTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG16410.1569511873199509No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAAGTGCCAGATGTGACAT16410.1569511873199509No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16200.15494267121165167No Hit
TCGTAGATCAGGACCTGAGGGGCTTTCCCCGGCTTCTGCTGATACCAATT16130.15427316584221862No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC15900.15207336248551No Hit
CCTACTATACTGGGAGCGCCTACTACAACCCCTCCCTCAAGAGTCGAGTC15780.15092563899505332No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15620.14939534100777774No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15560.1488214792625494No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT15220.14556959603958883No Hit
CATTTTACTTTCACCATCAACAACCTGCAGCCTGAAGATGTTGCAACATATTACTGTCAACAGTGGCATAGTGTC14590.13954404771469126No Hit
GGACCAAGGTGGCCATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14560.1392571168420771No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14380.13753553160639204No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC14320.13696166986116373No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14030.13418800475922674No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13850.1324664195235417No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG13840.132370775899337No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13670.13074483428785671No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13020.12452799871454968No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12850.1229020571030694No Hit
AACCAGTACTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTCT12820.12261512623045522No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT12750.12194562086102216No Hit
CACTATGCCACTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGG12570.12022403562533715No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12480.11936324300749464No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12430.11888502488647103No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12370.11831116314124268No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAAGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12320.11783294502021907No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12290.11754601414760489No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACTTGAGAGCCAGAG12250.11716343965078599No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12240.11706779602658128No Hit
CTACTATACTGGGAGCGCCTACTACAACCCCTCCCTCAAGAGTCGAGTCACCATCTCCGTTGACACGTCCACGAA12240.11706779602658128No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12210.11678086515396711No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12030.11505927991828208No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11910.11391155642782541No Hit
ATCTACAATAGTAGGCACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGCAC11880.11362462555521123No Hit
CTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGCACCTACTATACTGGGAGCGC11860.1134333383068018No Hit
GTGTCCGTCCAATGTTCACTTTCGGCCCTGGGACCAAGGTGGCCATCAAA11810.11295512018577816No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11760.11247690206475455No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAC11730.1121899711921404No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC11720.11209432756793566No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11650.11142482219850261No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11640.11132917857429789No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11630.11123353495009315No Hit
ACCTTGATGGGACCCCTGCTTCCAAATTGGATGCATCGTAGATCAGGACCTGAGGGGCTTTCCCCGGCTTCTG11530.11027709870804593No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11530.11027709870804593No Hit
GATCAGGACCTGAGGGGCTTTCCCCGGCTTCTGCTGATACCAATTTAATT11450.10951194971440814No Hit
ACTATACTGGGAGCGCCTACTACAACCCCTCCCTCAAGAGTCGAGTCACC11420.10922501884179396No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11380.10884244434497509No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11350.10855551347236089No Hit
AGTCAACACATTAACAACCAATTAAATTGGTATCAGCAGAAGCCGGGGAA11190.10702521548508534No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA11120.10635571011565227No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11100.10616442286724283No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACCATCTCCGTTGACACGTCCACGAACCAGTACTCCCTGAAGCTGAG11100.10616442286724283No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11000.10520798662519558No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10970.10492105575258143No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10900.10425155038314836No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10890.10415590675894362No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10830.1035820450137153No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAAGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC10740.10272125239587279No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10530.1007127362875736No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10510.10052144903916416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)