FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045548
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGT44150.42226660086385326No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42730.4086852062267825No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36850.3524467551944052No Hit
CTCTATTACTGTGCGAAAACACGGGGTAACTACGACTTTGGTTGGGGGGCCTTTGACTCCTGGGGCCAGGGAAGC34640.33130951424516136No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG34070.32585782766549215No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31930.3053900920856814No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31330.299651474633398No Hit
GTGGCATAGTGTCCGTCCAATGTTCACTTTCGGCCCTGGGACCAAGGTGG26540.25383817863933555No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26220.25077758266478445No Hit
CTATGCCACTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGTTGTTGATGGTGAAAGTAAAATGTGTC25400.2429348054799971No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAACACATTAACAACCAATTAAATTGGTATCAGCAGAAG24480.23413559205316256No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24210.23155321419963504No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23490.22466687325689494No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23080.2207454846645013No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22160.21194627123766677No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22160.21194627123766677No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21470.20534686116754083No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAACACATTAACAA20990.20075596720571412No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20490.19597378599547796No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGTTGTTGATGGTGAAAGTAAAATGTGTCCCAGATCCACTTCCG20220.19339140814195044No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20050.19176546653047016No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19840.18975695042217094No Hit
ATATTACTGTCAACAGTGGCATAGTGTCCGTCCAATGTTCACTTTCGGCC19630.18774843431387178No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19410.18564427458136787No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19200.18363575847306868No Hit
GATCTACGATGCATCCAATTTGGAAGCAGGGGTCCCATCAAGGTTCAGCGGAAGTGGATCTGGGACACATTTTA18790.17971436988067502No Hit
ACATATTACTGTCAACAGTGGCATAGTGTCCGTCCAATGTTCACTTTCGGCCCTGGGACCAAGGTGGCCATCAAA18690.1787579336386278No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18580.17770585377237583No Hit
CTATTACTGTGCGAAAACACGGGGTAACTACGACTTTGGTTGGGGGGCCTTTGACTCCTGGGGCCAGGGAAGC18130.17340189068316328No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17940.17158466182327353No Hit
GTGCAGCCACAGTTCGTTTGATGGCCACCTTGGTCCCAGGGCCGAAAGTGAACATTGGACGGACACTATGCCA17740.1696717893391791No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17710.1693848584665649No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17370.16613297524360432No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17020.162785448396439No Hit
CAGTAATAGAGAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA16920.16182901215439177No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16900.1616377249059823No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGTTGTTGATGGTGAAAGTAAAATGTGTCCCAGATCCACTTCCG16800.16068128866393508No Hit
GGCATAGTGTCCGTCCAATGTTCACTTTCGGCCCTGGGACCAAGGTGGCCATCAAACGAACTGTGGCTGCACCAT16400.15685554369574614No Hit
CCTTGGTCCCAGGGCCGAAAGTGAACATTGGACGGACACTATGCCACTGTTGACAGTAATATGTTGCAACATCTT16320.15609039470210836No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15950.15255158060653362No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAAGTGCCAGATGTGACAT15820.1513082134918722No Hit
GTATAGTAGGTGCTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG15800.15111692624346276No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC15530.14853454838993524No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGGCCACCTTGGTCC15460.14786504302050218No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15430.147578112147888No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15320.14652603228163605No Hit
CCTACTATACTGGGAGCGCCTACTACAACCCCTCCCTCAAGAGTCGAGTC14620.1398309785873054No Hit
GGACCAAGGTGGCCATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14430.13801374972741567No Hit
TCGTAGATCAGGACCTGAGGGGCTTTCCCCGGCTTCTGCTGATACCAATT14430.13801374972741567No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14220.13600523361911648No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAC14090.13476186650445507No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14040.13428364838343146No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT13880.1327533503961559No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13670.13074483428785671No Hit
CATTTTACTTTCACCATCAACAACCTGCAGCCTGAAGATGTTGCAACATATTACTGTCAACAGTGGCATAGTGTC13640.13045790341524255No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13480.12892760542796694No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13050.12481492958716386No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12990.12424106784193552No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAAGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12800.12242383898204577No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT12780.12223255173363633No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12650.12098918461897494No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12520.11974581750431354No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG12470.1192675993832899No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12390.11850245038965213No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC12160.11630264703294349No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACTTGAGAGCCAGAG12130.11601571616032932No Hit
GTGTCCGTCCAATGTTCACTTTCGGCCCTGGGACCAAGGTGGCCATCAAA12070.11544185441510098No Hit
CACTATGCCACTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGG11990.11467670542146319No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11770.11257254568895927No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11730.1121899711921404No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA11680.11171175307111676No Hit
ACCTTGATGGGACCCCTGCTTCCAAATTGGATGCATCGTAGATCAGGACCTGAGGGGCTTTCCCCGGCTTCTG11560.1105640295806601No Hit
AACCAGTACTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTCT11510.11008581145963649No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11430.1093206624659987No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11330.10836422622395145No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11300.10807729535133728No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11210.10721650273349478No Hit
GATCAGGACCTGAGGGGCTTTCCCCGGCTTCTGCTGATACCAATTTAATT11150.10664264098826644No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11130.106451353739857No Hit
CTACTATACTGGGAGCGCCTACTACAACCCCTCCCTCAAGAGTCGAGTCACCATCTCCGTTGACACGTCCACGAA11110.10626006649144754No Hit
AGTCAACACATTAACAACCAATTAAATTGGTATCAGCAGAAGCCGGGGAA11090.10606877924303809No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCCAG11080.10597313561883338No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11060.10578184837042393No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAAGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC11000.10520798662519558No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10930.10453848125576252No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10860.10386897588632947No Hit
CTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGCACCTACTATACTGGGAGCGC10800.10329511414110112No Hit
ATCTACAATAGTAGGCACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGCAC10760.10291253964428222No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10740.10272125239587279No Hit
ACTATACTGGGAGCGCCTACTACAACCCCTCCCTCAAGAGTCGAGTCACC10620.1015735289054161No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACCATCTCCGTTGACACGTCCACGAACCAGTACTCCCTGAAGCTGAG10560.10099966716018777No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT10500.10042580541495943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGG150.002545036167.6661414
CTTCGTT200.002759342566.29867669
AACGGAG13300.051.97510570
GCGTTAA200.007327078451.8065842
TGCGTTA200.007359710551.7483941
GTGGTAT2350.044.579621
ACGTCTG350.001195140240.4040553
GTATCAA15950.037.5020561
TGTTAGA12150.035.4139670
CAACGGA13900.035.29569669
AATGACG450.003787859831.9400759
TATCAAC18800.031.8092962
CGTGTCT12750.031.57753213
CCATTCG553.1415687E-431.55683745
GATTCAC1400.031.3803521
CGAACGT450.004235747731.2191450
GTATAAG652.6735037E-531.194671
AACCTCG652.6752521E-531.1916855
CCGTGTC12950.031.08984812
GTATAAT553.6763668E-430.7220251
Produced by FastQC (version 0.11.5)