FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260015
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48610.385789058066769No Hit
CTATTAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC46050.3654718396209569No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTATTAGTATCCCACAC43400.3444403439641592No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG43280.3434879743495117No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTA41570.3299167073407856No Hit
CCTATGTCCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG40320.31999619052154143No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32970.2616635516243854No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32300.2563461546092705No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30690.24356852894608397No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGT30500.24206061038955884No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27840.22094975059820718No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC27670.21960056031078995No Hit
GGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTCCACTGGTACCAGCAGAAGCCAGGCCAGGCCC26130.20737848358948108No Hit
CCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGAAATCCCT25210.20007698321051734No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23270.1846803411070503No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22680.17999785716836705No Hit
GTCCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG22490.17848993861184193No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT21730.17245826438574144No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGGACACTTTTACTT21660.17190271544386376No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTAT21130.1676964163125042No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21060.16714086737062653No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21030.16690277496696468No Hit
ATACTAATAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT20990.16658531842874885No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20820.16523612814133165No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20720.1644424867957921No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20320.16126792141363397No Hit
GTGATACAAGGCCGTGTCCTCAGCTCTCAGACTGTCCATTTGCAGATACA20020.15888699737701537No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19880.15777589949325999No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19650.15595052439851906No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGACTCAGC19570.15531561132208743No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGA19560.15523624718753348No Hit
GCCTTGTATCACTGTGTAAAAGATATCTCGGGTGGAACCCTCACGGGCTACTTTGACAACTGGGGCCAGGGAACC19460.15444260584199396No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19440.15428387757288603No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC19150.15198231767082138No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19080.1514267687289437No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19020.15095058392161997No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18780.1490458446923251No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18720.14856965988500137No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG18520.1469823771939223No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGACT18450.14642682825204462No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18020.1430141704662246No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17990.14277607806256273No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCAT17880.14190307258246926No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17840.14158561604425343No Hit
GCCTATGGTCTTACTATTCCAACTAATACCTGAGACCCACTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTG17800.14126815950603763No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17310.1373793169128939No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17200.1365063114328004No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17030.1351571211453832No Hit
GGCTCTGTGACCTCCTATGTCCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC16890.13404602326162784No Hit
TACTAATAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCC16700.13253810470510272No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGACTCAGCCAC16580.13158573509045526No Hit
GTCTGAGAGCTGAGGACACGGCCTTGTATCACTGTGTAAAAGATATCTCG16460.13063336547580784No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16400.13015718066848408No Hit
CTCCTGTGCAGCCTCTGGACTCACCTTTGATGATTATGCCATGCACTGGGTCCGGCAAGCTCCAGGGAAGGGC16250.1289667186501748No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16150.12817307730463526No Hit
GCACAGGCTCTGTGACCTCCTATGTCCTGACTCAGCCACCCTCGGTGTCA16060.12745880009364968No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA16020.12714134355543386No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15810.12547469672980083No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15730.1248397836533692No Hit
TCTATGATGATAGCGACCGGCCCTCAGAAATCCCTGAGCGATTCTCTGGCTCCAACTCTGGCAACACGGCCACC15580.12364932163505991No Hit
GACTTAGTACAGCCTGGCAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGACTCACCTTTGATGATTATGCCATG15360.12190331067487292No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14940.11857001702360685No Hit
GGGTGGCCGTGTTGCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATTTCTGAGGGCCGGTCGCTATCATCATAGA14890.11817319635083709No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT14760.1171414626016357No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14700.11666527779431196No Hit
TTGTATCACTGTGTAAAAGATATCTCGGGTGGAACCCTCACGGGCTACTT14650.1162684571215422No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14620.11603036471788035No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14210.11277643520116824No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG14080.11174470145196684No Hit
GTACTAAGTCTCCCCCAGACTCCACCAGCTGCACTTCACACTGGACACCT14050.11150660904830498No Hit
ACCATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCCCTGTATCTG13870.11007805462633381No Hit
CTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGTATTAGTTGGAATAGTAAG13760.10920504914624031No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13750.10912568501168637No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13740.10904632087713241No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGA13690.10864950020436263No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13430.10658603270595984No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13410.10642730443685194No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13380.10618921203319007No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13310.1056336630913124No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13290.10547493482220449No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG13180.10460192934211102No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA13180.10460192934211102No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13150.10436383693844915No Hit
CTCCAGAGACAACGCCAAGAACTCCCTGTATCTGCAAATGGACAGTCTGAGAGCTGAGGACACGGCCTTGTATCA13120.10412574453478728No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12720.10095117915262913No Hit
ATACAAGGCCGTGTCCTCAGCTCTCAGACTGTCCATTTGCAGATACAGGG12680.10063372261441333No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12660.10047499434530541No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12650.10039563021075146No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12630.10023690194164356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)