FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260015
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43170.3426149688694182No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTATTAGTATCCCACAC40850.32420248965290094No Hit
CTATTAGTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC40460.3211072884052968No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37520.29777423284643434No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTA37480.29745677630821854No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG36770.29182192275488783No Hit
CCTATGTCCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG33930.269282508541565No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31350.2488065618266449No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29440.23364801212683975No Hit
GGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTCCACTGGTACCAGCAGAAGCCAGGCCAGGCCC27980.22206084848196253No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGT27580.21888628309980435No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27550.2186481906961425No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24430.19388658071530895No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23960.19015646639127312No Hit
CCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGAAATCCCT23250.1845216128379424No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT22670.17991849303381308No Hit
GTCCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG22140.17571219390245355No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22110.17547410149879167No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21830.17325190573128096No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGACTCAGC20780.16491867160311585No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20730.16452185093034608No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20720.1644424867957921No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20660.1639663019884684No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGA20220.16047428006809442No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20010.1588076332424614No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGGACACTTTTACTT19980.15856954083879954No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19510.1548394265147637No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19340.1534902362273465No Hit
ATACTAATAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT19060.1512680404598358No Hit
GCCTTGTATCACTGTGTAAAAGATATCTCGGGTGGAACCCTCACGGGCTACTTTGACAACTGGGGCCAGGGAACC19050.15118867632528185No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18990.15071249151795812No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18740.14872838815410927No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18430.14626809998293672No Hit
GTGATACAAGGCCGTGTCCTCAGCTCTCAGACTGTCCATTTGCAGATACA18390.14595064344472092No Hit
GTCTGAGAGCTGAGGACACGGCCTTGTATCACTGTGTAAAAGATATCTCG18350.14563318690650506No Hit
GGGTGGCCGTGTTGCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATTTCTGAGGGCCGGTCGCTATCATCATAGA18230.14468081729185764No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGACT18190.1443633607536418No Hit
GGCTCTGTGACCTCCTATGTCCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC17900.14206180085157716No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTAT17830.14150625190969948No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG17800.14126815950603763No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17740.1407919746987139No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC17700.14047451816049808No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17690.14039515402594413No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17660.14015706162228228No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17400.13809359412387948No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17370.13785550172021763No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA17160.1361888548945846No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16850.13372856672341202No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16800.13333174605064227No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16650.13214128403233294No Hit
GCCTATGGTCTTACTATTCCAACTAATACCTGAGACCCACTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTG16580.13158573509045526No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16440.1304746372066999No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15830.12563342499890873No Hit
CTCCTGTGCAGCCTCTGGACTCACCTTTGATGATTATGCCATGCACTGGGTCCGGCAAGCTCCAGGGAAGGGC15770.12515724019158503No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGACTCAGCCAC15720.12476041951881525No Hit
GCACAGGCTCTGTGACCTCCTATGTCCTGACTCAGCCACCCTCGGTGTCA15670.12436359884604549No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCAT15470.1227763161549664No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15090.11976047904191617No Hit
TACTAATAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCC14950.11864938115816082No Hit
GACTTAGTACAGCCTGGCAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGACTCACCTTTGATGATTATGCCATG14910.11833192461994502No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14750.11706209846708174No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14330.1137288048158157No Hit
TCTATGATGATAGCGACCGGCCCTCAGAAATCCCTGAGCGATTCTCTGGCTCCAACTCTGGCAACACGGCCACC14040.11142724491375103No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13950.11071296770276545No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13890.11023678289544173No Hit
TTGTATCACTGTGTAAAAGATATCTCGGGTGGAACCCTCACGGGCTACTT13850.10991932635722591No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA13830.10976059808811801No Hit
GTACTAAGTCTCCCCCAGACTCCACCAGCTGCACTTCACACTGGACACCT13730.10896695674257846No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13610.10801458712793102No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGA13580.10777649472426916No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13250.10515747828398869No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13150.10436383693844915No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13090.10388765213112541No Hit
CTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGTATTAGTTGGAATAGTAAG13070.10372892386201751No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12980.10301464665103194No Hit
CTCCAGAGACAACGCCAAGAACTCCCTGTATCTGCAAATGGACAGTCTGAGAGCTGAGGACACGGCCTTGTATCA12950.10277655424737006No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG12940.10269719011281613No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12880.10222100530549239No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12820.10174482049816869No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12710.10087181501807517No Hit
ACCATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCCCTGTATCTG12660.10047499434530541No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTCCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG12630.10023690194164356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)