FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175578
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTATACTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC83600.7111395415701893No Hit
GAGTATTAGTTACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGT45330.3855975528633574No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC45000.3827904230940014No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39210.33353805532257325No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36540.31082582355232913No Hit
GTATAATCATTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT35510.3020641760903998No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG34000.2892194307821344No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA31660.26931432878124634No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30580.2601273586269903No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTTACTGGTTGGCCTGGTATCAGCAGAAA29590.25170596931892225No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28710.2442202899339729No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA27250.2318008673180342No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT27010.22975931839486619No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25030.2129165397787301No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24600.20925876462472076No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23010.19573350300873274No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22680.19292637323937672No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22230.18909846900843671No Hit
CCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTAACTAATACTCTGA22070.18773743639299137No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21930.1865465328544767No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21490.182803693162002No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21130.17974136977725No Hit
ATTATACTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC21030.17889072439259665No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20640.17557320739244864No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC20390.1734465939308153No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20120.1711498513922513No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGATCCCA20030.17038427054606328No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19700.1675771407767073No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19470.16562065639200463No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTTA19110.16255833300725261No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19050.1620479457764606No Hit
ACCTTGATGGGATCCCACTTTGTAAACTAGACGCCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG18750.1594960096225006No Hit
GTATTAGTTACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT18590.15813497700705526No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTAACTA18220.1549875890838379No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18200.15481746000690724No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18080.15379668554532322No Hit
ATCATTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA17850.15184020116062055No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17530.1491181359297299No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTATTGCCAACAGTATAATCATTAC17510.14894800685279921No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17220.14648113523730452No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16940.1440993281602752No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16660.14171752108324587No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG16570.14095194023705787No Hit
TCTATAAGGCGTCTAGTTTACAAAGTGGGATCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT16150.13737922962151383No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16110.1370389714676525No Hit
CAGTATAATCATTACCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16070.1366987133137912No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG15910.13533768069834584No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15740.13389158354443517No Hit
GATCTATAAGGCGTCTAGTTTACAAAGTGGGATCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA15530.13210522823666315No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC15130.12870264669804982No Hit
GGTAATGATTATACTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATT14870.12649096869795112No Hit
GGCCAAAAGTGTACGGGTAATGATTATACTGTTGGCAATAATAAGTTGCA14800.1258955169286938No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGATCCCACTTTGTAAACTAGA14670.12478967792864447No Hit
GTATCAACGCAGAGTACGGGTAATGATTATACTGTTGGCAATAATAAGTTGCAAAATCATCAGGCTGCAGGCTG14490.12325851623626845No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTAGTTTACAAAGTGGGATCCCAT14180.12062151554384311No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14030.11934554746686311No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG13410.11407154608201243No Hit
GCCCCTAACCTCCTGATCTATAAGGCGTCTAGTTTACAAAGTGGGATCCCATCAAGGTTCAGCGGCAGTGGAT13350.11356115885122042No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13180.11211506169730974No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13170.11202999715884442No Hit
TTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC12690.1079468993125084No Hit
AACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA12640.10752157662018173No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12610.10726638300478572No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12590.10709625392785506No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12550.10675599577399374No Hit
GATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGC12410.10556509223547907No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12220.10394886600463772No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC12100.10292809154305371No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG11950.1016521234660737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)