FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196968
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAACTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA45200.3776207885256749No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42680.35656759412114614No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG38640.32281564753610786No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37700.3149624718455297No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA37150.3103675286223191No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30140.2518028886319434No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA29640.2476256675199337No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28770.24035730278503686No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26680.22289651853683642No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT26560.2218939854699541No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25150.21011422193408677No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGTTGGCTTTTT24090.20125851317662627No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT23740.19833445839821948No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23650.19758255859805776No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23570.1969142032201362No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23310.19474204824189117No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTCATGCTCTGGGTC21510.17970405223865635No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20560.17176733212583795No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGTACTGATTTCACACTGAA20520.17143315443687718No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20420.17059771021447523No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20330.16984581041431351No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTCATGCTCTGGGTCCCAGG20220.1689268217696714No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19770.16516732276886267No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTGATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA19650.16416478970198034No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19420.16224326799045588No Hit
CGTATACAGTGATGGAAACACCTTCTTGCATTGGTTTCAGCAGAGGCCAG19260.1609065572346128No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19140.15990402416773047No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19140.15990402416773047No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18930.1581495913006864No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18760.15672933612260312No Hit
CTCGTATACAGTGATGGAAACACCTTCTTGCATTGGTTTCAGCAGAGGCC18530.15480781441107866No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18520.15472426998883845No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18510.15464072556659827No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18460.1542230034553973No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17930.14979514907666705No Hit
GCTCCTGGGGCTGCTCATGCTCTGGGTCCCAGGCTACAGCGGGGATGTTGTGATGACTCAGTCTCCACTCTCC17480.14603565007585834No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATA17460.14586856123137795No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATCAGGTTTCTAACCGGGAC17060.1425267843417702No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16800.1403546293635252No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC16610.1387672853409615No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16540.13818247438528014No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16340.13651158594047627No Hit
GTTTGTACCTTGCAAGCAGAAATAAACCCCAACATCTTCAGCCTCCACTC16340.13651158594047627No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCGACTGGACCATGATGTGGGTCCGCCAGGCCCCAGGGAAGGGG16300.1361774082515155No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT15460.12915967678333926No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA15190.126903977382854No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14980.12514954451580995No Hit
GAAATATACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATATA14680.12264321184860415No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14540.12147358993724142No Hit
AGTTTGTACCTTGCAAGCAGAAATAAACCCCAACATCTTCAGCCTCCACT14510.12122295667052085No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14380.12013687918139833No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC14110.11788117978091311No Hit
GGTACAAACTGGCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT13950.11654446902507001No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13930.11637738018058963No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTCATGCTCTGGGTCCCAGGCTACAGCGGGGATGTTGTGATGA13920.11629383575834944No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13890.11604320249162885No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC13650.1140381363578642No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13220.11044572620153587No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13200.11027863735705548No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13110.10952673755689375No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12880.10760521584536929No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC12780.10676977162296737No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGTTGGC12770.10668622720072718No Hit
GCTCATGCTCTGGGTCCCAGGCTACAGCGGGGATGTTGTGATGACTCAGT12610.10534951644488408No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT12610.10534951644488408No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12580.1050988831781635No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12540.10476470548920272No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG12440.10392926126680077No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGTTGGCTTTTTCTTGT12380.10342799473335963No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC12320.10292672819991845No Hit
GTGATGGAAACACCTTCTTGCATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATCAGGTTT12150.10150647302183517No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA12140.10142292859959498No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT12100.1010887509106342No Hit
GGTCTAGTCAAAGCCTCGTATACAGTGATGGAAACACCTTCTTGCATTGGTTTCAGCAGAGGCCAGGCCAATCTC12010.10033685111047247No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA11980.10008621784375188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)