FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230154
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAAGTGGAATTACTTAGCCTGGTACCAGCAGAA75590.6144759111460841No Hit
CTCCTAAGCTGCTCATTTCCTGGGCGTCTACCCGGGAATCCGGGGTCCCT59300.48205346647655495No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58780.47782635344843No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56020.4553901381453054No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA53540.43523006062655567No Hit
GCTTAGGAGGCTGTCCTGGCTTCTGCTGGTACCAGGCTAAGTAATTCCACTTGTTGGAGCTGTATAAAACACT51240.41653321454061853No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42870.3484929529148383No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38870.3159766988523388No Hit
AGCCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCGTCTACCCGGGAA35760.29069531131874543No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35680.2900449862374955No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33970.2761442876257769No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33420.27167330269218326No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32990.2681778053804646No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32320.2627313328249959No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29120.23671832957499628No Hit
GTTTATTACTGTCAGCAATATTATGATACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAGCGAA29040.2360680044937463No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28980.23558026068280885No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27870.2265570001804652No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27690.22509376874765272No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27610.22444344366640273No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27290.22184214334140276No Hit
GTCCTGGCTTCTGCTGGTACCAGGCTAAGTAATTCCACTTGTTGGAGCTG27040.21980987746249656No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC27000.21948471492187158No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26770.21761503031327784No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26730.21728986777265286No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26200.21298146410937166No Hit
GTGCAGCCACAGTTCGCTTGAATTCCACCTTGGTCCCTTGGCCGAACGTC25860.2102175825140592No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA25710.2089982229867155No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25030.20347045979609057No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA24970.2029827159851531No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24620.2001375437546844No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGAATTCCACCTTGGTCC24530.19940592803827814No Hit
ATATTATGATACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAGCGAACTGTGGCTGCACCAT22190.18038391941171594No Hit
GTCAGGGACCCCGGATTCCCGGGTAGACGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTGGCTTCTGCTGGTA21570.17534390003202852No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21270.17290518097734106No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA21210.17241743716640354No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20790.16900323048984112No Hit
TTATGATACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAGCGAACTGTGGCTGCACCATCTGTCTT20780.16892193985468484No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20520.1668083833406224No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20510.16672709270546615No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20120.16355675793437244No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20090.1633128860289037No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20020.16274385158280996No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20000.16258127031249747No Hit
CTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCC19730.16038642316327872No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC19560.1590044823656225No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATACT19460.15819157601406003No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19300.15689092585156006No Hit
GTATCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT18900.1536393004453101No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC18510.1504689656742164No Hit
TCCGAGGAGTATCATAATATTGCTGACAGTAATAAACTGCCACATCTTCA18360.14924960614687266No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT18260.14843669979531018No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17250.14022634564452907No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17170.13957602056327909No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16780.13640568579218537No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG16700.13575536071093539No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT16690.13567407007577914No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16690.13567407007577914No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAAGTGGAATTA16550.13453600118359166No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16290.13242244466952918No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16240.13201599149374793No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATACTCCTCGGACGTT16160.13136566641249794No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16070.1306340506960917No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15970.12982114434452924No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15900.12925210989843547No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA15840.128764366087498No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15750.12803275037109174No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15710.12770758783046676No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15550.12640693766796676No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15380.12502499687031055No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15330.12461854369452931No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15190.12348047480234181No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15140.12307402162656059No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15110.12283014972109182No Hit
TCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC15040.12226111527499808No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15040.12226111527499808No Hit
GCTCCAACAAGTGGAATTACTTAGCCTGGTACCAGCAGAAGCCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGG14870.12087917447734185No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14750.11990368685546689No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG14370.11681464271952942No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC14280.11608302700312319No Hit
GCCACAGTTCGCTTGAATTCCACCTTGGTCCCTTGGCCGAACGTCCGAGG14180.1152701206515607No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14060.1142946330296857No Hit
GGACCAAGGTGGAATTCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC13990.11372559858359198No Hit
GCCTGGTACCAGCAGAAGCCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCGTCTACCCGGGAATCCGGGGTC13980.11364430794843572No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13810.1122623671507795No Hit
GCTCATTTCCTGGGCGTCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT13680.11120558889374826No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13530.10998622936640454No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13520.10990493873124828No Hit
GAATTACTTAGCCTGGTACCAGCAGAAGCCAGGACAGCCTCCTAAGCTGC13490.10966106682577953No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13470.10949848555546703No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13290.10803525412265456No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATGATACTCCTC13280.1079539634874983No Hit
GTACCAGCAGAAGCCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCGTCTACCCGGGAATCCGGGGTCCCTGA13220.10746621967656082No Hit
TTTCCTGGGCGTCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACT12910.10494620998671711No Hit
GCCAAGGGACCAAGGTGGAATTCAAGCGAACTGTGGCTGCACCATCTGTC12850.10445846617577963No Hit
ATGATACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAGCGA12750.10364555982421712No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG12720.10340168791874839No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12600.1024262002968734No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12490.10153200331015466No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12450.10120684076952967No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12390.10071909695859219No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12370.10055651568827968No Hit
CCTCGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG12350.10039393441796719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)