FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358965
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCTACATGGGATGACAGTCTGAATGGTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA78460.577351145908835No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG75680.5568944012538954No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC66220.4872826010971585No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC62420.4593201443745792No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG57710.42466141512106637No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG54080.3979499104097604No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT53080.39059136916697634No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC49540.3645421331675209No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46380.34128914284032336No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCGACCATTCAGA46230.3401853616539057No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCG46150.339596678354483No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC45120.33201738087441546No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG43320.3187720066374042No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT41970.30883797595964574No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC40820.3003756535304441No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG39670.2919133311012425No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA39630.29161898945153114No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG39140.288013304242567No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT38400.28256798372290676No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC38340.2821264712483397No Hit
GTGCTACATGGGATGACAGTCTGAATGGTCGAGTGTTCGGCGGAGGGACC37480.2757981257795455No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG37470.27572454036711763No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA36430.26807165747462225No Hit
ATGTAGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG36280.2669678762882046No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC35450.26086028705669384No Hit
GTATTGCCATAGATGAAGACTTTGGGGGCCGTTCCTGGGAGCTGCTGATA34430.25335457498905417No Hit
CCCCTGAGGGCCGCTGAGTATTGCCATAGATGAAGACTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGGTGA34390.2530602333393428No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC33490.2464375462208372No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACGTGGAGCCAGAGAATCGGTCAGGGACC33080.24342054431129573No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC32910.24216959230002247No Hit
GAAGTAATACTGTCACCTGGTATCAGCAGCTCCCAGGAACGGCCCCCAAA32870.2418752506503111No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG32710.24069788405146564No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT32310.237754467554352No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31950.23510539270694977No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30800.22664307027774813No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29440.21663545418756186No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCTACATGGGATGACAGTCTGAATGGTCGAGTGTTCGGCGGAGGGAC29080.21398637934015963No Hit
GCTGATACCAGGTGACAGTATTACTTCCGATGTTGGAGCTGCTTCCAGAA28340.2085410588204994No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG27940.20559764232338582No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA27840.2048617881991074No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT27530.20258064041384435No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCGACCATTCAGAC27290.20081459051557618No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACA26810.19728249071903986No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26690.19639946576990577No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26610.19581078247048306No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26400.1942654888094984No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC25380.1867597767418587No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG24930.18344843318260587No Hit
GGATGACAGTCTGAATGGTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCC24410.17962199173635818No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24160.17778235642566217No Hit
CCCAGGAACGGCCCCCAAAGTCTTCATCTATGGCAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG23950.17623706276467754No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23670.174176671216698No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACGTGGAGCCAGAGAATCGGTC23640.17395591497941448No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTCACCTGGTATCAGCAGCTCCCAGGAACGGCCCC23590.17358798791727528No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG23410.17226345049357414No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC23330.17167476719415145No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC23330.17167476719415145No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT23140.17027664435802248No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT23080.16983513188345542No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT22920.16865776528460996No Hit
TGGCAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGGC22870.1682898382224708No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG22710.16711247162362533No Hit
GATGAAGACTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGGTGACAG22530.1657879341999242No Hit
GTCTTCATCTATGGCAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATT22370.16461056760107876No Hit
TGGGATGACAGTCTGAATGGTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCC22310.16416905512651173No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22310.16416905512651173No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22280.16394829888922818No Hit
GTCATCCCATGTAGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCC21900.16115205321697026No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21870.16093129697968675No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21650.15931241790627426No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT21320.15688409929615554No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC21250.15636900140916066No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC20670.1521010474883459No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCTA20570.1513651933640675No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19870.1462142144941187No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19830.14591987284440733No Hit
GAGTATTGCCATAGATGAAGACTTTGGGGGCCGTTCCTGGGAGCTGCTGA19780.14555194578226813No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA19750.1453311895449846No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19460.14319721258457724No Hit
GTAATACTGTCACCTGGTATCAGCAGCTCCCAGGAACGGCCCCCAAAGTCTTCATCTATGGCAATACTCAGCGGC19290.14194626057330395No Hit
CTGTCATCCCATGTAGCACAGTAATAATCAGCCTCATCCTCAGACTGGAG19190.14121040644902555No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC18910.139150014901046No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC18800.13834057536433977No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG18780.1381934045394841No Hit
GCTTGGTCCCTCCGCCGAACACTCGACCATTCAGACTGTCATCCCATGTAGCACAGTAATAATCAGCCTCATCCT18560.13657452546607163No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACT18310.1347348901553756No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTA18090.1331160110819631No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18020.13260091319496822No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGA17930.13193864448311768No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG17510.12884805716114836No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC17200.12656690937588533No Hit
ATGGCAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGG17180.12641973855102964No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17030.12531595736461204No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAG16860.12406500535333877No Hit
GGTATCAGCAGCTCCCAGGAACGGCCCCCAAAGTCTTCATCTATGGCAATACTCAGCGGCCCTCAGGGGTCC16710.12296122416692115No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16640.12244612627992627No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG16300.1199442222573797No Hit
GTACAGACTGGGCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGG16250.11957629519524049No Hit
GTCAGGGACCCCTGAGGGCCGCTGAGTATTGCCATAGATGAAGACTTTGGGGGCCGTTCCTGGGAGCTGCTGATA16020.11788383070940017No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTCAC16020.11788383070940017No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15950.11736873282240529No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15910.11707439117269393No Hit
GACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACGTGGAGCC15780.116117780811132No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15730.11574985374899281No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT15610.11486682879985873No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15460.11376304761344111No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT15440.11361587678858542No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15420.11346870596372977No Hit
ACATGGGATGACAGTCTGAATGGTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGG15410.11339512055130191No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA15370.11310077890159054No Hit
CAGTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACC15340.11288002266430704No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACT15230.11207058312760079No Hit
GTGCCAGACGTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG15180.11170265606546158No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAG15130.11133472900332238No Hit
GTTCCTGGGAGCTGCTGATACCAGGTGACAGTATTACTTCCGATGTTGGA15130.11133472900332238No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC15040.11067246029147183No Hit
GTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG15010.11045170405418829No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG14940.10993660616719342No Hit
GTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGGC14910.1097158499299099No Hit
GGTATCAGCAGCTCCCAGGAACGGCCCCCAAAGTCTTCATCTATGGCAAT14900.10964226451748206No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG14610.10750828755707467No Hit
TTATTACTGTGCTACATGGGATGACAGTCTGAATGGTCGAGTGTTCGGCG14510.10677243343279628No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT14510.10677243343279628No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14500.10669884802036844No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14160.10419694399782188No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG13960.10272523574926506No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13820.1016950399752753No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC13790.10147428373799179No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG13780.10140069832556395No Hit
AGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13730.10103277126342473No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC13700.10081201502614122No Hit
GCAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACG13600.10007616090186283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3200.062.0992870
TACGTAT1101.8189894E-1260.21748470
AACGACG200.00656202753.27247658
TCTCGTA2150.052.84470438
CGTATGC2750.052.82433341
TCGACGT3000.050.0695729
CTCGACG3000.050.0474228
TCGTATG2700.049.8793840
GACTCGA3050.049.17983226
CTTATAC2850.048.9974561
ATGCCGT2850.048.73122444
CGACGTC3100.048.47945430
GCCGTCT2750.048.1261346
GACGTCA3200.047.0165231
CCGTCTT2850.046.49698347
ATCTCGT2450.046.30674737
AGACTCG3300.045.44069725
ACGAGAC3300.045.41728622
CGAGACT3400.045.08665523
TCCGAGC3400.044.08148613
Produced by FastQC (version 0.11.5)