FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358965
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCTACATGGGATGACAGTCTGAATGGTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA74730.5499037870732506No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC70420.5181884743168514No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC65330.4807334993910807No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG61380.45166726148208375No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG58350.4293708815164482No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT56800.41796514259013295No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG50240.3696931120374697No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC44550.32782301236602857No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43960.32348147303278596No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC43590.3207588127729559No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCG41100.30243604507842364No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG40460.29772657868304186No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC40460.29772657868304186No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCGACCATTCAGA39990.2942680642989334No Hit
GTGCTACATGGGATGACAGTCTGAATGGTCGAGTGTTCGGCGGAGGGACC39380.2897793541408351No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC39020.2871302792934328No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT38640.28433403362117493No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37850.2785207860393755No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT36670.2698377073728904No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG36520.2687339261864728No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36320.267262217937916No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36040.26520182638993645No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG35200.25902065174599787No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC34570.25438477076304394No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA34190.251588525090786No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACA33290.2449658379722804No Hit
GAAGTAATACTGTCACCTGGTATCAGCAGCTCCCAGGAACGGCCCCCAAA32900.24209600688759458No Hit
GTATTGCCATAGATGAAGACTTTGGGGGCCGTTCCTGGGAGCTGCTGATA32120.23635634471822303No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31990.23539973435666114No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT31930.2349582218820941No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC31920.23488463646966626No Hit
CCCCTGAGGGCCGCTGAGTATTGCCATAGATGAAGACTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGGTGA31890.23466388023238274No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG31860.2344431239950992No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCTACATGGGATGACAGTCTGAATGGTCGAGTGTTCGGCGGAGGGAC31500.23179404914769694No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG30300.22296379965635613No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30140.22178643305751067No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA29370.21612035630056697No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC28520.20986559624420054No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27670.2036108361878341No Hit
ATGTAGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG27250.20052024886586484No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26710.19654663659476146No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT26410.19433907422192626No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTCACCTGGTATCAGCAGCTCCCAGGAACGGCCCC26260.19323529303550863No Hit
GCTGATACCAGGTGACAGTATTACTTCCGATGTTGGAGCTGCTTCCAGAA26190.19272019514851377No Hit
GGATGACAGTCTGAATGGTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCC25790.18977677865140016No Hit
GTCTTCATCTATGGCAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATT25490.18756921627856493No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25120.18484655601873484No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC25020.18411070189445644No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT24890.18315409153289453No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24860.18293333529561098No Hit
CCCAGGAACGGCCCCCAAAGTCTTCATCTATGGCAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG24740.1820503103464769No Hit
TGGCAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGGC24270.17859179596236843No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCGACCATTCAGAC23640.17395591497941448No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23610.17373515874213097No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG23450.1725577921432855No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23210.17079174224501736No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22950.1688785215218935No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22910.16858417987218213No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG22900.1685105944597543No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22500.1655671779626407No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22460.16527283631292933No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACT22250.16372754265194467No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC22150.16299168852766627No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT22090.16255017605309924No Hit
GATGAAGACTTTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGGTGACAG21840.1607105407424032No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA21730.15990110120569698No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC21660.1593860033187021No Hit
TGGGATGACAGTCTGAATGGTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCC21550.15857656378199586No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACGTGGAGCCAGAGAATCGGTC21280.15658975764644417No Hit
GTAATACTGTCACCTGGTATCAGCAGCTCCCAGGAACGGCCCCCAAAGTCTTCATCTATGGCAATACTCAGCGGC21180.15585390352216577No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT21050.15489729316060386No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC20890.1537199265617584No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20830.15327841408719134No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC20320.1495255580533715No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20200.14864253310423742No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20120.1480538498048147No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTA19210.1413575772738812No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCTA19100.14054813773717498No Hit
GAGTATTGCCATAGATGAAGACTTTGGGGGCCGTTCCTGGGAGCTGCTGA19070.14032738149989146No Hit
GTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG18800.13834057536433977No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACT18770.13811981912705626No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC18510.1362065984039324No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC18230.13414620685595288No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG18080.13304242566953528No Hit
GGTATCAGCAGCTCCCAGGAACGGCCCCCAAAGTCTTCATCTATGGCAATACTCAGCGGCCCTCAGGGGTCC18010.1325273277825404No Hit
GTCATCCCATGTAGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCC17600.12951032587299893No Hit
ATGGCAATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGG17290.1272291780877359No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17210.12664049478831316No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17060.12553671360189556No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17000.1250952011273285No Hit
GCTTGGTCCCTCCGCCGAACACTCGACCATTCAGACTGTCATCCCATGTAGCACAGTAATAATCAGCCTCATCCT16850.12399141994091092No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGA16760.12332915122906035No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC16750.1232555658166325No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAG16580.12200461380535922No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG16570.12193102839293138No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16280.11979705143252402No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAG15940.11729514740997744No Hit
GTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGGC15900.11700080576026609No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15790.11619136622355986No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC15690.11545551209928143No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTCAC15650.11516117044957008No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC15620.11494041421228655No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG15510.11413097467558031No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15390.11324794972644622No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15380.11317436431401838No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15370.11310077890159054No Hit
GGTATCAGCAGCTCCCAGGAACGGCCCCCAAAGTCTTCATCTATGGCAAT15330.1128064372518792No Hit
GTCAGGGACCCCTGAGGGCCGCTGAGTATTGCCATAGATGAAGACTTTGGGGGCCGTTCCTGGGAGCTGCTGATA15230.11207058312760079No Hit
CTGTCATCCCATGTAGCACAGTAATAATCAGCCTCATCCTCAGACTGGAG15220.11199699771517295No Hit
CAGTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACC15210.1119234123027451No Hit
GTACAGACTGGGCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGG15140.11140831441575022No Hit
GTGCCAGACGTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG15140.11140831441575022No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15130.11133472900332238No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15060.11081963111632749No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC14990.1103045332293326No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14970.11015736240447695No Hit
TTATTACTGTGCTACATGGGATGACAGTCTGAATGGTCGAGTGTTCGGCG14860.10934792286777069No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT14720.10831772709378093No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC14490.1066252626079406No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG14410.10603657930851788No Hit
ACATGGGATGACAGTCTGAATGGTCGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGG14210.10456487105996108No Hit
GTTCCTGGGAGCTGCTGATACCAGGTGACAGTATTACTTCCGATGTTGGA14210.10456487105996108No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT14180.10434411482267755No Hit
GGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTCACCTGGTATCAGCAG13860.10198938162498665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCA200.007771556351.0414516
CGTCTAA305.8157544E-446.77034850
GTGGTAT2850.040.570471
ACGTCTA350.001253142540.0174749
GGACGTC350.001275480439.8739447
GTATCAA28650.037.3905531
ATAGGCA750.002071850536.121970
TACGTAT750.002071850536.121970
GGCTTAA502.0151665E-434.03766323
TATCAAC32550.032.6944052
CAACGCA34400.030.8395635
AACGCAG34650.030.5234176
ACCCGAG900.005061456530.10158570
ATCAACG36400.029.329753
TCAACGC36650.029.1296834
GTACGTA904.772761E-528.65524169
ACGCAGA37100.028.427567
CGCAGAG37300.028.0947828
CGGACGT500.007368719727.8759746
AGAGTAC37600.027.69780311
Produced by FastQC (version 0.11.5)