FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446424
Sequences flagged as poor quality0
Sequence length21-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGCTACTGCTTGTAAATGAGCTG73440.5077349380264707No Hit
CTCCTGCACTGGTGGTTATAACTATGTCTCCTGGTTCCAACAGCACCCAG73190.5060065375021432No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC72320.4999917036774832No Hit
TCTCTGGACTCCAGGCTGAAGACGAGGCTGATTATTACTGCAGCTCATTT59190.4092161081397986No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG54500.37679131430341306No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGTGGTTATAACTATGTC52280.3614431176473842No Hit
GACCTACGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGCTACTGCTTGTAAATGAGCTGCAGTAATAAT50010.34574924088648973No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45200.312494814798427No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42390.29306759290498496No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG41750.2886428875627064No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG41520.287052759080325No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG39720.2746082753051664No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38870.2687317135224526No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG38630.26707244901909816No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTC38330.26499836838990504No Hit
GATTATTACTGCAGCTCATTTACAAGCAGTAGCACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCGTAGGT35900.2481983152934409No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT35640.24640077874814023No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG34300.2371365519377444No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA34090.23568469549730925No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31840.220129090778361No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT31290.2163266096248403No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC31250.21605006554094788No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31150.21535870533121687No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30240.20906732742266443No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30070.20789201506612168No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC29800.20602534249984789No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29220.20201545328340792No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT29170.2016697731785424No Hit
GTCGATCACCATCTCCTGCACTGGTGGTTATAACTATGTCTCCTGGTTCC28930.2000105086751879No Hit
GTAATAATCAGCCTCGTCTTCAGCCTGGAGTCCAGAGATGGTCAGGGAGG28490.19696852375237134No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27750.19185245820036173No Hit
CAGTAGCACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA26780.18514626416597071No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26780.18514626416597071No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG25020.17297832447470451No Hit
GTGCTACTGCTTGTAAATGAGCTGCAGTAATAATCAGCCTCGTCTTCAGC24600.1700746115938342No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24490.16931411536313004No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGTGGTTATAACTATGT24440.16896843525826452No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23940.16551163420960935No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23740.16412891379014727No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGTGGTTATAA23610.16323014551749693No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23390.16170915305608866No Hit
CCCAAGTGCTACTGCTTGTAAATGAGCTGCAGTAATAATCAGCCTCGTCT23120.15984248048981486No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22710.15700790362991765No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21990.1520301101198542No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAACAC21540.14891898917606455No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21460.14836590100827973No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT21340.1475362687566025No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21130.1460844123161673No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT20160.1393782182817763No Hit
GGTTATAACTATGTCTCCTGGTTCCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCACTAAT19830.13709672958966387No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC19710.13626709733798667No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19650.13585228121214804No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19540.1350917849814439No Hit
GATCAGGACTGCACACAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGG19320.13357079252003562No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19190.13267202424738528No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19070.13184239199570805No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18770.12976831136651493No Hit
GTCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18430.12741768665342942No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18350.1268645984856446No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18250.12617323827591356No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT17930.12396088560477425No Hit
GGTTCCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC17410.12036581251417289No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17310.11967445230444186No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17000.11753123565427565No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16920.11697814748649082No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT16850.1164941953396791No Hit
ATAATAAACAGCCGTCTCCTCGGCTTTCAGACTGTTCATTTGCAGATACA16700.11545715502508254No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16590.1146966587943784No Hit
CCCCTGGGGGCCGATTAGTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGAACCAGGAGA16530.11428184266853979No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16490.11400529858464738No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16190.11193121795545428No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC16120.11144726580864256No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16070.11110158570377703No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15900.10992627334723429No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGTGCTACTGCTTGTAAATGAGCTGCAGTAATAATCAGCCTCGTCTTCAG15880.10978800130528807No Hit
ACCTACGCGGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACGC15590.10778305669706809No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGCTA15450.10681515240344464No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15410.10653860831955222No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAACACC15100.10439539166938602No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC15070.10418798360646671No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14890.10294353522895085No Hit
ACCTACGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGCTACTGC14850.10266699114505842No Hit
CCCCAGGGGTCTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGACTCCAGG14830.10252871910311223No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC14820.10245958308213912No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14800.10232131104019292No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT14590.10086945459975775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)