FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353280
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCGGTGACCCCGGGACAGACG35170.25988708914637026No Hit
ATACAGGGGCCTGGCCTGGTTTCTGGCGGAACCACTGCACACCTTTATTT32370.23919661858595415No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31590.23343284464412392No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29950.2213141404587373No Hit
CCCCTGTATTGGTCATCCATGATGATGGCGACCGGCCCTCCGGGATCCCT29470.21776720264838023No Hit
GCCTTGGGCTGACGCAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCACTTCCAGTATCCCACAC28170.20816091274532986No Hit
GTATTGGTCATCCATGATGATGGCGACCGGCCCTCCGGGATCCCTGAGCG28060.20734807283045636No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGCAGGACGGTCAGCTTGGTCCCTC28000.20690470560416177No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27700.20468786947268858No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27650.20431839678410973No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25630.18939170016552379No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23230.1716570111137385No Hit
GTATTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGGGACAATT22440.16581934263419248No Hit
GACCAATACAGGGGCCTGGCCTGGTTTCTGGCGGAACCACTGCACACCTT22250.1644153464175928No Hit
CCTTTATTTCGAATATTGTCTCCCCCACAGGTGATCGTGGCCGTCTGTCCCGGGGTCACCGACACCGAGGGTGG22200.16404587372901397No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21610.1596860960037834No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21500.1588732560889099No Hit
GTGCAGGGAGAAGGGCCTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21410.15820820524946796No Hit
CCCCGGGACAGACGGCCACGATCACCTGTGGGGGAGACAATATTCGAAAT20550.15185327500591156No Hit
GAGAAGGGCCTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20540.15177938046819578No Hit
TCCATGATGATGGCGACCGGCCCTCCGGGATCCCTGAGCGAATCTCTGGGTCCAACTCTGGGAACACGGCCACC19940.14734570820524948No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19910.14712402459210216No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19540.1443899266966186No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT19470.14387266493260817No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19350.14298593048001892No Hit
AAATAAAGGTGTGCAGTGGTTCCGCCAGAAACCAGGCCAGGCCCCTGTAT19300.14261645779144005No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18970.14017793804681958No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18910.13973457082052493No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18810.13899562544336722No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18690.13810889099077797No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18620.13759162922676757No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18360.1356703712461575No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18250.134857531331284No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18210.1345619531804209No Hit
CCTGTGGGGGAGACAATATTCGAAATAAAGGTGTGCAGTGGTTCCGCCAG18100.13374911326554742No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18080.13360132419011586No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17960.1327145897375266No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17840.13182785528493735No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17580.12990659730432724No Hit
ATCCCACACCTGACAATAATAGTCGGCCTCATCTCCGCCTTCGACCCTGC17560.1297588082288957No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC17500.1293154410026011No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17340.12813312839914873No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCGGTGACCCCGGGACAGACGGCCACGATCAC17310.12791144478600142No Hit
GGGTGGCCGTGTTCCCAGAGTTGGACCCAGAGATTCGCTCAGGGATCCCG17130.12658134310711752No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA16990.1255468195790967No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16930.1251034523528021No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16540.12222156538188697No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16530.12214767084417119No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCCTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16400.12118704185386617No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16250.12007862378812957No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGGCGGAGATGAGGCCGACTAT16200.11970915109955071No Hit
GTCTGAGACGTGAGGACACGGCTGTCTATTTCTGTGTCAGGCGCGCTAAGGACCGGGTCATATCAGTCCTTGACT16110.11904410026010877No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC16110.11904410026010877No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16010.11830515488295106No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCCTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15890.11741842043036178No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15840.11704894774178293No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15800.11675336959091984No Hit
GATATGACCCGGTCCTTAGCGCGCCTGACACAGAAATAGACAGCCGTGTC15590.11520158429888863No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15260.11276306455426816No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14970.11062012296051076No Hit
GGTCATATCAGTCCTTGACTCCTGGGGCCAGGGATCCCTCGTCACCGTCT14840.10965949397020573No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGCAGGACGGTCAGC14670.1084032868290376No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14540.10744265783873255No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14490.1070731851501537No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCG14420.10655592338614331No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC14110.10426519271695435No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG14110.10426519271695435No Hit
CTATTATTGTCAGGTGTGGGATACTGGAAGTGATCATGTGGTCTTCGGCG13960.10315677465121778No Hit
CTCCAGGGACAATTCCAAGAACACCTTGTATCTGCAAATGTACAGTCTGAGACGTGAGGACACGGCTGTCTATTT13940.10300898557578625No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT13860.10241782927406005No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13710.10130941120832349No Hit
GAAATAGACAGCCGTGTCCTCACGTCTCAGACTGTACATTTGCAGATACA13650.10086604398202885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT650.057.9465937
CGCGTAT453.1152013E-853.47591839
AGGGGGG3350.050.69502370
TCGCGTA507.262861E-848.027438
GTGGTAT2000.047.397281
ACGAGAC4200.046.7506922
CGCGTTT306.4235355E-445.83650239
TCGCGTT306.490332E-445.74038338
CGAGACT4300.044.88611623
GGTATCA7750.044.1209681
CGTTTGC551.4655416E-743.92611341
TTATACA4650.042.954472
ATCTCGT1700.042.29791637
CCCACGA4650.042.22019219
TATACAC4800.041.6121443
CTTATAC4900.041.4553451
CACGAGA4800.040.9023221
TCCGAGC4900.040.06757713
CATCGCG1200.039.8779736
CGTATGC1650.039.74267241
Produced by FastQC (version 0.11.5)