FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004686961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778734
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28100.36084208471698936No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCACTGCTACTATCCCACAC27870.3578885729915478No Hit
CTTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG25610.3288671099502526No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23290.29907516558927694No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCACTG22750.2921408337121533No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20610.2646603333102189No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19510.2505348424494115No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18360.23576728382220372No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16440.2111118815924308No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15990.20533327169482773No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15670.2012240379898656No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14600.1874837877888984No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14590.18735537423561832No Hit
CCCCTGTGTTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT14450.18555758448969736No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14210.18247565921097575No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13910.17862325261257375No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13780.17695387641993285No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13550.17400036469449132No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13480.17310146982153085No Hit
GTGTTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG13320.17104685296904976No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13090.16809334124360822No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACT13050.16757968703048795No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12920.16591031083784707No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGGCGGGGATGAGGCCGACTAT12760.163855693985366No Hit
CCTTTACTTCCAATGTTGTATCCCCCACAGGTAATCGTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG12720.1633420397722457No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12680.16282838555912543No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12280.15769184342792278No Hit
GGGTGGCCGTGCTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA12180.1564077078951221No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGC12080.1551235723623214No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11930.1531973690631204No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11690.15011544378439878No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA11670.14985861667783865No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAGCACGG11630.14934496246471837No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGCCTTCGACCCTGC11620.14921654891143832No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11530.1480608269319177No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11390.14626303718599676No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG11370.14600621007943662No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCACTGC11200.14382317967367547No Hit
GCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGCCACCCTCGGTGTCA11170.14343793901383528No Hit
CTATTACTGTCAGGTGTGGGATAGTAGCAGTGATCATGTGGTTTTCGGCG10980.140998081501514No Hit
CGATTACCTGTGGGGGATACAACATTGGAAGTAAAGGTGTGCATTGGTATCAGCAGAAGCCAGGCCAGGCCC10840.13920029175559306No Hit
GGCTCTGTGACCTCTTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTAC10750.13804456977607246No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10510.13496264449735085No Hit
GGCCAGGCCCCTGTGTTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG10380.13329326830470994No Hit
GGATAGTAGCAGTGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC10240.13149547855878901No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGCCAC10140.13021134302598833No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT9970.12802831262022718No Hit
GGATACAACATTGGAAGTAAAGGTGTGCATTGGTATCAGCAGAAGCCAGG9970.12802831262022718No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCTTA9500.12199287561606403No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9270.11903936389062247No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9240.11865412323078227No Hit
TAGTAGCAGTGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCC9200.118140469017662No Hit
CAGTAATAGTCGGCCTCATCCCCGCCTTCGACCCTGCTGATGGTCAGGGT9130.11724157414470153No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9120.11711316059142146No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9070.11647109282502113No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTACCTGTGGGGGATACAACATTGGAAGTAAAGGTGTG9020.11582902505862078No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8890.11415964886597992No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG8860.11377440820613971No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8800.11300392688645931No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC8780.11274709977989916No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATGCACACCTTTACTT8760.11249027267333904No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8590.11030724226757788No Hit
CTGCTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGCCTTC8530.10953676094789748No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8490.10902310673477722No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8430.10825262541509682No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGCCTTCGACCCTGCTGATGGTCAGGGTGGCCGTG8360.10735373054213634No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCACGATTACCTGTGGGGGATACAA8360.10735373054213634No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8350.10722531698885626No Hit
AAGTAAAGGTGTGCATTGGTATCAGCAGAAGCCAGGCCAGGCCCCTGTGT8310.10671166277573602No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8300.10658324922245593No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8290.10645483566917587No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8260.10606959500933566No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8220.1055559407962154No Hit
GTGTGGGATAGTAGCAGTGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG8190.10517070013637521No Hit
CTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGG8140.10452863236997485No Hit
GTAATAGTCGGCCTCATCCCCGCCTTCGACCCTGCTGATGGTCAGGGTGG8090.10388656460357452No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8010.10285925617733396No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCA7860.10093305287813296No Hit
TCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAGCACGGCCACC7840.10067622577157283No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7790.1000341580051725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)