FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778734
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25580.3284818692904124No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCACTGCTACTATCCCACAC24200.3107607989377631No Hit
CTTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG23300.299203579142557No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22160.28456443406862936No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20680.26555922818317934No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCACTG19730.25335994062157297No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17320.2224122742810767No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16760.21522111529739293No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16290.2091856782932298No Hit
GGGTGGCCGTGCTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA16080.2064889936743484No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15790.20276500062922642No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14870.1909509537274602No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14630.1878690284487386No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14490.18607123870281764No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14480.18594282514953758No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13830.1775959441863332No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACT13680.1756697408871322No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13320.17104685296904976No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13110.16835016835016833No Hit
CCCCTGTGTTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT12990.16680920571080754No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12930.16603872439112713No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGC12880.1653966566247268No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12510.1606453551533643No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12220.15692136210824237No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11940.15332578261640048No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA11750.1508859251040792No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11260.14459366099335588No Hit
GTGTTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG11260.14459366099335588No Hit
CCTTTACTTCCAATGTTGTATCCCCCACAGGTAATCGTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG11210.14395159322695555No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAGCACGG10960.14074125439495386No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10900.13997077307527347No Hit
GCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGCCACCCTCGGTGTCA10610.1362467800301515No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10550.1354762987104711No Hit
GGCTCTGTGACCTCTTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTAC10500.13483423094407077No Hit
CTATTACTGTCAGGTGTGGGATAGTAGCAGTGATCATGTGGTTTTCGGCG10430.13393533607111027No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGGCGGGGATGAGGCCGACTAT10380.13329326830470994No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10060.12918403459974778No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10040.12892720749318767No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG9940.127643071960387No Hit
GGATAGTAGCAGTGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC9850.1264873499808664No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCTTA9630.1236622518087049No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT9600.12327701114886469No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9580.12302018404230455No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCTTATGTGCTGACTCAGCCAC9540.12250652982918428No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC9510.12212128916934409No Hit
GGCCAGGCCCCTGTGTTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG9510.12212128916934409No Hit
CGATTACCTGTGGGGGATACAACATTGGAAGTAAAGGTGTGCATTGGTATCAGCAGAAGCCAGGCCAGGCCC9450.12135080784966368No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCACTGC9420.12096556718982347No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9410.12083715363654342No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG9360.12019508587014308No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGATACCAATGCACACCTTTACTT9220.11839729612422215No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGCCTTCGACCCTGC9160.11762681480454173No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9030.11595743861190086No Hit
GTGTGGGATAGTAGCAGTGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG8870.11390282175941976No Hit
CTGCTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGCCTTC8840.11351758109957957No Hit
GGATACAACATTGGAAGTAAAGGTGTGCATTGGTATCAGCAGAAGCCAGG8830.11338916754629952No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8750.11236185912005896No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8690.11159137780037856No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTC8660.11120613714053836No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8640.11094931003397823No Hit
TAGTAGCAGTGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCC8590.11030724226757788No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8500.10915152028805727No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8490.10902310673477722No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC8320.10684007632901606No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCACGATTACCTGTGGGGGATACAA8230.10568435434949545No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8220.1055559407962154No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCA8180.10504228658309513No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8040.1032444968371742No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7920.10170353419781336No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTACCTGTGGGGGATACAACATTGGAAGTAAAGGTGTG7840.10067622577157283No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7820.1004193986650127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT259.489955E-486.7496470
AGGTCTA352.5363972E-548.3772745
TCGTTAT350.00141517139.03772433
AACCGTA350.001476576238.701827
ATCGGCT400.002443746734.92717748
GTGGTAT1700.033.907641
ACGGGAT400.00284283233.86626416
GCGGGAT400.002844622433.86191613
GTATCAA14650.033.0975571
TATAATG553.6203687E-430.8013612
AATCGTT450.00466336130.61050839
CTCGTTA450.00488800130.3175132
TATCAAC16200.029.9077422
AAAACCG1000.006155523528.91654670
ATCAACG16950.028.3753833
GTATAAT855.0640865E-627.9239371
TCAACGC17350.027.7176364
TTATTCC500.00799613327.4116336
CGTTATT500.00808524927.34943634
CAACGCA17650.027.2447665
Produced by FastQC (version 0.11.5)