FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935314
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT32220.3444832430606192No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA26660.2850379658595937No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC26470.2830065625020047No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA26440.28268581460343795No Hit
CCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCT26390.28215123477249354No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23250.24857962138918055No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22860.24440989870781363No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC22070.23596353737889092No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA21460.22944166344136835No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20930.22377511723335694No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20380.21789473909296772No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTCAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAG20020.2140457643101675No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19930.21308352061446745No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG19430.2077377223050227No Hit
GCACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAGCATATTTATCC18970.20281958786033355No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCACTGAGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG18730.20025360467180006No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTGAGGTCTTCGGAACTGGGACCAAGGT18230.19490780636235533No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17980.19223490720763292No Hit
CTATTACTGTCAGGCGTGGGACAGCAGCACTGAGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC17750.18977583998528838No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17240.18432312570965473No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16910.18079489882542119No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16830.17993957109591002No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC16810.17972573916353224No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTC16310.1743799408540875No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT16140.1725623694288763No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG15220.16272610053949796No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG14970.16005320138477558No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTCAGTGCTG14490.15492123500770863No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14370.1536382434134419No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT14320.1531036635824974No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTCAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAGC13980.149468520732075No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC13820.14775786527305268No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT13680.14626104174640817No Hit
GGCCAGTCCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGG13570.14508496611833033No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13400.1432673946931191No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13360.14283973082836351No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG13250.14166365520028568No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13140.14048757957220784No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13110.14016683167364113No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCA13090.13995299974126338No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12610.13482103336419643No Hit
AGCCAGGCCAGTCCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCC12180.1302236468180739No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAC11760.12573317623814034No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC11650.1245571006100625No Hit
GTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCTGAGCG11610.12412943674530692No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11490.12284644515104018No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11230.12006663003012892No Hit
GTGTATTACTGTGCGAGAGGGGCTACTATGGTTCGGGGAGTCGCGGAAAA11190.11963896616537335No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10690.1142931678559286No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG10520.11247559643071739No Hit
CCTCAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATA10480.11204793256596182No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10400.11119260483645065No Hit
GGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTGAGGTCTTCGGAACTGGGACCAAGGTCACCGT10160.10862662164791716No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC9930.1061675544255726No Hit
GCACAGGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCC9890.10573989056081701No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9810.10488456283130586No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC9800.10477764686511695No Hit
GCACTGAGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCC9790.10467073089892806No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9790.10467073089892806No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9740.10413615106798359No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9720.10392231913560579No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9570.10231857964277237No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9560.10221166367658348No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAGCTGACT9400.10050100821756115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAATCG200.007697154351.16455515
CGTATGC950.050.92579741
ATCTCGT1000.044.727637
TCGTATG1150.042.03043440
CGAGCTT457.995336E-539.8265964
ATGCCGT1150.039.21286844
CTCGCTA702.4125256E-838.9950323
CCGTCTT1250.038.965347
GTATCAA27600.038.313671
GCCGTCT1200.037.64449346
GTGGTAT2400.036.9541551
CGAGACT1400.036.5578423
ACGAGAC1400.036.5539322
TCGCTAT754.4303306E-836.3953624
CTTATAC1600.036.2434961
GCTAATC1250.035.65326733
TATCAAC29800.035.1417432
GTCGATC400.002455055534.895149
ACGGGTC701.0639269E-634.10970316
TCCGAGC1650.033.07430613
Produced by FastQC (version 0.11.5)