FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935314
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT29620.31668509185150656No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC28100.3004338649907945No Hit
CCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCT24630.263334024723248No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA24130.2579882264138033No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA22340.2388502684659911No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC22030.23553587351413538No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG21250.22719642815140156No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA20090.21479417607348975No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19710.21073136935831177No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19570.20923454583166723No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG18670.19961210887466668No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTCAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAG18630.19918444500991112No Hit
GCACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAGCATATTTATCC17890.1912726635119329No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17760.18988275595147724No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCACTGAGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG17090.18271938621682132No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17060.18239863831825462No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACTGAGGTCTTCGGAACTGGGACCAAGGT16600.17748050387356545No Hit
CTATTACTGTCAGGCGTGGGACAGCAGCACTGAGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC16470.17609059631310983No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16320.1744868568202764No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG16180.17299003329363186No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC15970.17074479800366507No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15950.1705309660712873No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15580.16657507532229818No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15520.1659335795251648No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14890.15919787365526444No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTC14120.15096534425871952No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG14110.15085842829253063No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT14030.1500031005630195No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT13870.14829244510399717No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13610.1455126299830859No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTCAGTGCTG13500.14433655435500806No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13460.14390889049025246No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12660.1353556131951409No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12610.13482103336419643No Hit
GGCCAGTCCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGG12470.1333242098375519No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11940.12765766362954045No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCTCAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAGC11860.1268023359000293No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA11710.12519859640719588No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCA11640.1244501846438736No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT11470.12263261321866238No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG11200.11974588213156223No Hit
AGCCAGGCCAGTCCCCTGTGCTGGTCATCTATCAAGATAGCAAGCGGCCC11070.11835597457110661No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC11010.11771447877397323No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10950.11707298297683986No Hit
GGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAC10830.11578999138257312No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10810.11557615945019532No Hit
GTGCTGGTCATCTATCAAGATAGCAAGCGGCCCTCAGGGATCCCTGAGCG10490.11215484853215069No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC10370.11087185693788397No Hit
GTGTATTACTGTGCGAGAGGGGCTACTATGGTTCGGGGAGTCGCGGAAAA10350.11065802500550617No Hit
CCTCAGTGCTGCTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATA9780.10456381493273915No Hit
GCACAGGATCCGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCC9600.10263932754133907No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9560.10221166367658348No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9550.1021047477103946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2200.049.606251
GTATCAA25400.040.2806661
TATCAAC26250.037.772672
CGTTCGG1550.037.370721
ACGTTCG1600.036.20867520
ATCAACG27550.036.0985263
TCAACGC27950.035.4543654
CAACGCA28050.035.2065665
AACGCAG28450.034.5918776
GTATTAT2200.034.1042981
TATAAGA807.911876E-834.0732882
TTATCCG1700.034.0532510
ACGCAGA30150.032.6414227
AATCATC2000.032.5227232
GGCAGTA2000.032.35750614
CGCAGAG30950.032.237818
AGAGTAC32100.031.08286911
AGGTGAT10550.030.85880570
TATACTG2100.030.8100815
AATGGTA5300.030.71324370
Produced by FastQC (version 0.11.5)