FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234620
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACACCCGTGTCCGCGGCGGTCACAGAGTTCAGTCTCAGGGAGA64560.522913933032026No Hit
GTCTATGGGAGGTTCGACTATTATTGGACTTGGGTCCGCCAGACCCCAGG59020.47804182663491607No Hit
GTATATTACTGTGCGAGAGGCCTGAGGGGGGCGTCGGTGACTAGGACGGTGACGGCCAGGAAACAGTTCTACTAC40370.32698320130890474No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT38810.31434773452560305No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC36410.29490855485898493No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG35480.28737587273817045No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGG33280.26955662471043723No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32730.2651018127035039No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA32500.2632388913187863No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC31670.25651617501741425No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC31130.2521423595924252No Hit
GACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACTCCATAGAGACCA30430.24647259885632825No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG28650.2320552072702532No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC28420.23019228588553564No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG27250.22071568579805934No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25820.20913317458003275No Hit
ACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACTCCATAGAGACCAT25570.20710826003142668No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC25520.20670327712170544No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25350.20532633522865334No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCACGATGACATGCCAAGGAGACAGTCTCAGAAC24700.20006155740227763No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24090.19512076590367886No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24060.19487777615784613No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG23890.19350083426479403No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACTCCATAGAGACCATCGATGTCTCGGGA22990.18621114188981225No Hit
CCCATAGACTGCGCAGGTGAGGGACAGGGTCTCCGAGGGCCTCAATAGCCCTGCGCCCCACTGTTCTATCTGCAC22400.181432343555102No Hit
CTATTACTGTCAGTCCCGAGACATCGATGGTCTCTATGGAGTCTTCGGAT22330.1808653674814923No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC21160.17138876739401596No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21130.17114577764818323No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21080.17074079473846204No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20890.16920185968152143No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20090.16272213312598208No Hit
GGACTGGAGTGGCTCGGAGAAATCAATCATAGTGGAATCACCGCATACAACCCGTCCCTCAGGAGACGGGTCAGT19950.16158818097876268No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19430.15737635871766212No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19420.15729536213571788No Hit
CCATAGAGACCATCGATGTCTCGGGACTGACAGTAATAGTCAGCCTCATC19200.15551343733294457No Hit
GACTATTACTGTCAGTCCCGAGACATCGATGGTCTCTATGGAGTCTTCGG19170.1552704475871118No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19130.15494646125933487No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG18800.15227357405517486No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18770.15203058430934216No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGCTTCTTCTGAGTTGACTCAGGA18480.14968168343295912No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA18400.14903371077740518No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18280.1480617517940743No Hit
CAGTAATATACACCCGTGTCCGCGGCGGTCACAGAGTTCAGTCTCAGGGAGACCTGGCTCTTGGACGCGTCAA18260.1478997586301858No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17970.1455508577538028No Hit
CTATGGAGTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT17840.14449790218852765No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17830.1444169056065834No Hit
CCTTGGGGCTGGTCGGGGATGCTGATGAGACGACGACCGTGGTCCCTTGG17780.1440119226968622No Hit
GTCCCTCACCTGCGCAGTCTATGGGAGGTTCGACTATTATTGGACTTGGGTCCGCCAGACCCCAGGGAAGGGA17690.14328295345936404No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17690.14328295345936404No Hit
GGGCTATTGAGGCCCTCGGAGACCCTGTCCCTCACCTGCGCAGTCTATGG17630.14279697396769855No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGCTTCTTCTGAGTTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA17570.1423109944760331No Hit
CTCTTTGCATAGGTTCTGTGGCTTCTTCTGAGTTGACTCAGGACCCTGCT17440.14125803891075794No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17270.13988109701770585No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC17110.138585151706598No Hit
GACTAGGACGGTGACGGCCAGGAAACAGTTCTACTACCCTATCGACGTCTGGGGCCAAGGGACCACGGTCGTCGT16990.13761319272326708No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16970.1374511995593786No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16630.13469731577327437No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16490.13356336362605498No Hit
GTCTCAGGGAGACCTGGCTCTTGGACGCGTCAACTGAAAGACTGACCCGT16450.133239377298278No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16430.13307738413438952No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16400.13283439438855682No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG16120.13056649009411803No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15800.1279745994719023No Hit
GTCTCAGAACCTACTATGCAAGCTGGTACCAGAAGAAGCCAGGCCAGGCC15580.12619267466912895No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT15370.12449174644829988No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15340.12424875670246716No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15300.12392477037469019No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACTCCATAGAGAC15020.12165686608025142No Hit
ATATTACTGTGCGAGAGGCCTGAGGGGGGCGTCGGTGACTAGGACGGTGACGGCCAGGAAACAGTTCTACTACC14980.12133287975247445No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG14700.11906497545803568No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14610.1183360062205375No Hit
GTCAAGGACCTCCTGCACAAGAACATGAAACACCTGTGGCTCTTCCTCCTACTGGTGGCAGCTCCCAGATGGGTC14560.11793102331081629No Hit
GGCCAGGCCCCTCTCCTTGTCTTTTATCATAAAAACAGTAGGCCCTCAGG14460.11712105749137386No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTCAGTCCCGA14460.11712105749137386No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14140.11452916686915812No Hit
GTTCAGTCTCAGGGAGACCTGGCTCTTGGACGCGTCAACTGAAAGACTGA14100.11420518054138114No Hit
GTCTGTGGCCTTGGGACAGACAGTCACGATGACATGCCAAGGAGACAGTCTCAGAACCTACTATGCAAGCTGGTA14060.1138811942136042No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14020.11355720788582722No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG13920.1127472420663848No Hit
ATATACACCCGTGTCCGCGGCGGTCACAGAGTTCAGTCTCAGGGAGACCT13820.11193727624694237No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC13530.10958837537055936No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGATGAGACGACGACCGTGGTCCCTTGG13280.10756346082195331No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13160.10659150183862241No Hit
GGTGTATATTACTGTGCGAGAGGCCTGAGGGGGGCGTCGGTGACTAGGACGGTGACGGCCAGGAAACAGTTCTA13070.10586253260112424No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13020.10545754969140302No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12980.10513356336362605No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT12890.10440459412612788No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12800.1036756248886297No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG12740.10318964539696425No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12690.10278466248724302No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG12540.10156971375807941No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTG12490.10116473084835821No Hit
ACTCCATAGAGACCATCGATGTCTCGGGACTGACAGTAATAGTCAGCCTC12410.10051675819280426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTCG200.00750763951.48918535
TACAACG200.00765119451.2431032
TGGTATC1550.050.692112
GGTATCA6550.050.06961
GGAGAAA20750.045.7091770
GTGGTAT1950.042.0456241
GTATCAA17250.038.6179921
AACTGAA18900.038.082470
CTACGCT557.869767E-637.29641328
AATGGTA4950.036.69111670
TATCAAC19150.034.786442
TGGACCG501.9723321E-434.1620675
CGGAGAA18300.033.12309669
ATCAACG20700.032.1816643
CTTATAC851.3176214E-732.152541
TCAACGC20900.031.87374
ACTACGC652.4729374E-531.55338727
AGGGACT16500.031.48788553
CAACGCA21350.031.201895
TATTGTG450.004852757330.3638257
Produced by FastQC (version 0.11.5)