FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1234620
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGGAGGTTCGACTATTATTGGACTTGGGTCCGCCAGACCCCAGG68020.5509387503847337No Hit
GTAATATACACCCGTGTCCGCGGCGGTCACAGAGTTCAGTCTCAGGGAGA55110.44637216309471744No Hit
GTATATTACTGTGCGAGAGGCCTGAGGGGGGCGTCGGTGACTAGGACGGTGACGGCCAGGAAACAGTTCTACTAC38370.3107838849200564No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT35040.2838120231326238No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33390.2704475871118239No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGG32070.25975603829518396No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC31600.2559491989438046No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28970.23464709789246893No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28190.22832936450081806No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA28100.22760039526331988No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27820.22533249096888108No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC27030.218933760995286No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC26970.21844778150362054No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG26440.21415496266057574No Hit
GGGCTATTGAGGCCCTCGGAGACCCTGTCCCTCACCTGCGCAGTCTATGG26330.21326400025918907No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26170.2119680549480812No Hit
GACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACTCCATAGAGACCA25490.20646028737587274No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23650.19155691629813223No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23100.18710210429119892No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG22860.1851581863245371No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22850.18507718974259288No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22670.1836192512675965No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACTCCATAGAGACCATCGATGTCTCGGGA22640.18337626152176378No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22640.18337626152176378No Hit
ACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACTCCATAGAGACCAT22520.18240430253843287No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCACGATGACATGCCAAGGAGACAGTCTCAGAAC22190.1797314153342729No Hit
GTCCCTCACCTGCGCAGTCTATGGGAGGTTCGACTATTATTGGACTTGGGTCCGCCAGACCCCAGGGAAGGGA21380.1731706921967893No Hit
CTATTACTGTCAGTCCCGAGACATCGATGGTCTCTATGGAGTCTTCGGAT21220.17187474688568144No Hit
CCCATAGACTGCGCAGGTGAGGGACAGGGTCTCCGAGGGCCTCAATAGCCCTGCGCCCCACTGTTCTATCTGCAC21180.17155076055790447No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20910.16936385284540992No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20890.16920185968152143No Hit
GGACTGGAGTGGCTCGGAGAAATCAATCATAGTGGAATCACCGCATACAACCCGTCCCTCAGGAGACGGGTCAGT20810.1685538870259675No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19430.15737635871766212No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19360.15680938264405242No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGCTTCTTCTGAGTTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA19100.15470347151350214No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18670.15122061848989973No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18370.14879072103157245No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG18360.14870972444962824No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGCTTCTTCTGAGTTGACTCAGGA18220.14757577230240884No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18060.14627982699130096No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17630.14279697396769855No Hit
GACTATTACTGTCAGTCCCGAGACATCGATGGTCTCTATGGAGTCTTCGG17540.14206800473020037No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16850.13647924057604768No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16760.13575027133854953No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16750.13566927475660528No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC16720.13542628501077256No Hit
CTATGGAGTCTTCGGATCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT16700.13526429184688404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16620.13461631919133013No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16420.13299638755244528No Hit
CTCTTTGCATAGGTTCTGTGGCTTCTTCTGAGTTGACTCAGGACCCTGCT16410.13291539097050104No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT16320.13218642173300285No Hit
CCATAGAGACCATCGATGTCTCGGGACTGACAGTAATAGTCAGCCTCATC16280.1318624354052259No Hit
GACTAGGACGGTGACGGCCAGGAAACAGTTCTACTACCCTATCGACGTCTGGGGCCAAGGGACCACGGTCGTCGT16130.13064748667606227No Hit
CAGTAATATACACCCGTGTCCGCGGCGGTCACAGAGTTCAGTCTCAGGGAGACCTGGCTCTTGGACGCGTCAA16060.13008051060245257No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16050.12999951402050833No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG15890.12870356870940047No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15780.1278126063080138No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA15710.1272456302344041No Hit
GTCTCAGAACCTACTATGCAAGCTGGTACCAGAAGAAGCCAGGCCAGGCC15680.12700264048857138No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15350.12432975328441141No Hit
CCTTGGGGCTGGTCGGGGATGCTGATGAGACGACGACCGTGGTCCCTTGG15300.12392477037469019No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15260.12360078404691321No Hit
GTCAAGGACCTCCTGCACAAGAACATGAAACACCTGTGGCTCTTCCTCCTACTGGTGGCAGCTCCCAGATGGGTC15130.12254782848163806No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15030.12173786266219566No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14940.12100889342469748No Hit
GTCTGTGGCCTTGGGACAGACAGTCACGATGACATGCCAAGGAGACAGTCTCAGAACCTACTATGCAAGCTGGTA14890.12060391051497626No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGATCCGAAGACTCCATAGAGAC14780.1197129481135896No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG14660.1187409891302587No Hit
GGTGTATATTACTGTGCGAGAGGCCTGAGGGGGGCGTCGGTGACTAGGACGGTGACGGCCAGGAAACAGTTCTA14620.11841700280248173No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14440.11695906432748539No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGATGAGACGACGACCGTGGTCCCTTGG14330.1160681019260987No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT14190.11493414977887934No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14010.11347621130388297No Hit
GGCCAGGCCCCTCTCCTTGTCTTTTATCATAAAAACAGTAGGCCCTCAGG13850.1121802659927751No Hit
ATATTACTGTGCGAGAGGCCTGAGGGGGGCGTCGGTGACTAGGACGGTGACGGCCAGGAAACAGTTCTACTACC13770.11153229333722117No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13660.1106413309358345No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13390.10845442322333998No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTCAGTCCCGA13390.10845442322333998No Hit
CTCCAGTCCCTTCCCTGGGGTCTGGCGGACCCAAGTCCAATAATAGTCGA13260.10740146765806484No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTG13170.10667249842056664No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC13090.10602452576501271No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT13040.10561954285529151No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12940.10480957703584909No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA12690.10278466248724302No Hit
CACTTGCACTGCTGCCTACCCCGAGTCCAAGACCCCGCTAACCGCCACCC12570.10181270350391212No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG12450.10084074452058123No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGAT208.9587855E-568.2947125
TGGTATC2000.049.4555132
AACTGAA17250.046.68868670
GTGGTAT2200.046.5174261
GGTATCA7050.046.4514431
ATGGCAA1853.6379788E-1241.28228870
GGAGAAA21700.041.27364370
GTATCAA17600.036.8262941
AATGGCA1451.8189894E-1236.1677869
AATGGTA5750.032.60158570
AAATGGC1305.638867E-1132.52181268
TATCAAC19750.032.2939572
CAACTGA16300.031.63756469
TACGTAC1208.731149E-1131.30047612
CCTACGT450.004321531.09202651
GTATAGG450.004885209730.322471
ATCAACG21150.030.156293
ATACGTA1251.4188117E-1030.04845811
CAACGCA21600.030.0041795
TCAACGC21500.029.6641754
Produced by FastQC (version 0.11.5)