FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1038137
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGCGGTAGAGTCTTCGG51040.49164994600905276No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT36710.35361421469420706No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC33630.32394568346952285No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA32990.3177807938643936No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC32930.31720283546391276No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC28180.2714477954258446No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG27740.26720943382231827No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25140.24216456980148093No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT23220.22366990098609338No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC22020.21211073297647612No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC21890.21085848977543428No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21220.20440462097006465No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG20830.20064789136693906No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20760.19997360656637803No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20630.1987213633653362No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG20460.19708381456397372No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20420.19669850896365318No Hit
GTGTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTAAACATAATTACTT20420.19669850896365318No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC20400.19650585616349286No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19500.18783648015627993No Hit
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGAAGATACA18960.1826348545519522No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT18880.18186424335131104No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCTACCGCTCAGGCTGTCATCCCATG18370.17695159694722373No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC18010.17348384654433857No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17810.17155731854273568No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17680.1703050753416938No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17650.1700160961414534No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC17430.16789691533969023No Hit
CCCCTGAGGGCCGCTGATTATTCCTATAGATGAGGAGTGTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTAAA17250.16616304013824765No Hit
GATGAGGAGTGTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTAAACAT16840.16221365773496177No Hit
CTCTTGTTCTGGAAGCAACTCCAACATCGGAAGTAATTATGTTTACTGGTACCAGCAGCTCCCAGGAACGGCCCC16660.16047978253351916No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16580.15970917133287804No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16470.15864958093199646No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT15940.15354428172774884No Hit
CTCCTCATCTATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATT15900.15315897612742826No Hit
GCCCCCACACTCCTCATCTATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT15670.15094346892558497No Hit
CAGTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTTGAAGATA15640.15065448972534454No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15350.14786102412302038No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG15250.14689776012221895No Hit
GATTATTCCTATAGATGAGGAGTGTGGGGGCCGTTCCTGGGAGCTGCTGG15040.1448749057205359No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14860.14314103051909335No Hit
GTGTATTACTGTGTGGGCTGGAACAACCTCGCGGGGATTGACTACTGGGGCCAGGGAACCCAGGTCACCATCTC14710.1416961345178912No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCTACCGCTCAGG14680.14140715531765075No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14260.13736144651428472No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCTACCGCTCAGGC14240.13716879371412444No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG13860.133508390511079No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT13800.1329304321105981No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13350.12859574410699165No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13290.12801778570651082No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13280.12792145930643065No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12920.12445370890354548No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12650.1218528961013816No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12150.11703657609737443No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12040.11597698569649284No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11940.1150137216956914No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC11880.11443576329521056No Hit
GGATGACAGCCTGAGCGGTAGAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC11870.1143394368951304No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG11870.1143394368951304No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAACTCCAACATCGGAAGTAATTAT11730.11299086729400841No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG11660.1123165824934474No Hit
GTATTACTGTGTGGGCTGGAACAACCTCGCGGGGATTGACTACTGGGGCCAGGGAACCCAGGTCACCATCTCCT11630.11202760329320698No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11490.11067903369208495No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11460.11039005449184454No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11460.11039005449184454No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11430.11010107529160408No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC11420.11000474889152395No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC11290.10875250569048209No Hit
GTGCAGCATGGGATGACAGCCTGAGCGGTAGAGTCTTCGGAACTGGGACC10930.10528475528759691No Hit
GTAGAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCC10840.10441781768687562No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG10840.10441781768687562No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10810.10412883848663519No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC10800.10403251208655506No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10730.10335822728599404No Hit
GCATGGGATGACAGCCTGAGCGGTAGAGTCTTCGGAACTGGGACCAAGGT10710.10316557448583376No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10670.10278026888551317No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA10630.10239496328519261No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG10620.10229863688511245No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG10590.10200965768487204No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC10580.1019133312847919No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG10430.10046843528358974No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAA200.00742745151.6286238
TGGTATC3150.047.623252
GTGGTAT3400.044.1406861
GGTATCA10100.042.8891751
GTATCAA23800.037.1183051
TCTGGAG8950.034.80565670
CGGTACC400.002526902434.69085345
GGTACGA400.00275270234.0906318
TAGACCT400.002758561234.075854
GACAGGT1550.033.0084341
TATCAAC27150.032.1474882
TCCGCTC450.00420499531.26566156
ATCAACG28550.030.6831023
TCAACGC29050.030.2635784
AGGAGTA803.0339233E-629.824993
CAACGCA29750.029.5514955
AACGCAG30250.029.063046
GTCCTAG33100.028.8901970
ATTAGAG953.5605262E-728.7037493
CACATGT605.7471264E-428.46510528
Produced by FastQC (version 0.11.5)