FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156972
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTCCCCCTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT85830.741850278139834No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC72460.6262900052896699No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54320.46950142267920053No Hit
GATCTATGCTGCATCCACTTTGCAAACTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA40280.34815017130924514No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37320.32256614680389845No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36950.31936814374073014No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36940.31928171122550936No Hit
CAGTATAATAGTTCCCCCTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT33090.28600519286551446No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31150.2692372849126859No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC31100.26880512233658205No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29470.25471662235559717No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28970.2503949965945589No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27440.23717082176578172No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGGTCATTTGCAGATACA26840.23198487085253575No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC26680.23060195060900351No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26430.22844113772848437No Hit
GTCCCTGATCTATGCTGCATCCACTTTGCAAACTGGGGTCCCATCAAAGT25840.22334161933045918No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGCCATTAGGAATTATTTAGCCTGGTTTCAGCAGAAA25440.21988431872162853No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25410.21962502117596622No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC25090.2168591806889017No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24750.21392047517139567No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23860.2062279813167475No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23050.19922694758386544No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21650.18712639545295823No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21540.1861756377855298No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT21490.18574347520942597No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG21480.18565704269420522No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21440.18531131263332218No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21120.18254547214625766No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20190.17450724823072641No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20040.1732107605024149No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19950.17243286786542802No Hit
GATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAT19520.1687162697109351No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19190.16586399670864982No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18360.15869009794532624No Hit
ATGCTGCATCCACTTTGCAAACTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA17870.1544549046995087No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT17390.15030614396891195No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16970.14667597832963977No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT16770.14494732802522448No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGTGCCAGATGTGACAT16550.14304581269036762No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTCC16310.14097143232506923No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCA16200.14002067465764081No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16160.13967494459675775No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG16060.1388106194445501No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15360.13276034337909645No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15280.13206888325733035No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCT15240.13172315319644728No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15220.13155028816600575No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15170.13111812558990193No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC15140.13085882804423962No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15130.13077239552901884No Hit
GTTTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA15130.13077239552901884No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14770.12766082498107129No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14580.12601860719187674No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA14450.12489498449400677No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13900.12014119615686464No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13810.11936330351987774No Hit
GTGTATTACTGTGCGAGTGGGGCGTATAGCAGTGGCTGGACACCGGGGGA13730.11867184339811163No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTCCCCCTTCACTTT13730.11867184339811163No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13650.11798038327634548No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13500.11668389554803402No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13150.1136587575153072No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13090.1131401624239826No Hit
GTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCAGTTTGCAAAGT13030.11262156733265802No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12920.11167080966522959No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12890.11141151211956728No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12850.11106578205868421No Hit
ACTTATTACTGCCAACAGTATAATAGTTCCCCCTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA12770.1103743219369181No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12630.10916426672382737No Hit
AGTCAGGCCATTAGGAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTATG12440.10752204893463282No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12310.10639842623676285No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12200.10544766856933442No Hit
GCCCCTAAGTCCCTGATCTATGCTGCATCCACTTTGCAAACTGGGGTCCCATCAAAGTTCAGCGGCAGTGGAT11990.10363258574969834No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11940.10320042317359453No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11860.1025089630518284No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11790.10190393544528303No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTG11660.10078031274741307No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11640.10060744771697154No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11600.10026171765608848No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11600.10026171765608848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTT200.00457491658.3559268
CGTTTCG200.00766805251.2143725
TACGTAT702.8057157E-547.55599670
GGTATCA7000.043.8088341
GTATCAA18450.039.336931
TGGTATC1800.035.955632
TATCAAC20250.035.6612552
GTGGTAT1850.034.9944461
ATCAACG20600.034.8884963
TCAACGC20750.034.6362954
TATATGA400.002763705834.063232
CAACGCA21550.033.515
TCTGGAG11050.032.53604570
AACGCAG22400.032.2481736
AGAGTAC23500.031.05955311
CGCAGAG23500.030.9023788
CAGAGTA23900.030.68243610
GCAGAGT24450.029.9818579
GAGGGAC23350.029.76177830
GTCATAG704.1920688E-529.205891
Produced by FastQC (version 0.11.5)