FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980460
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC45890.4680456112437019No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33100.33759663831262876No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCAAGTCATGCTGATTGTTAATGAACTG32120.3276013299879648No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31700.3233176264202517No Hit
CTCCTGCACTGCTACCACTGTTGACGTTGGTAGTTATAAGTATGTCTCCTGGTATCATCAATACCCAGGCAAAGC28530.29098586377822655No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26500.2702812965342798No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24530.25018868694286356No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24340.24825082104318383No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24320.248046835159007No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24060.2453950186647084No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23800.2427432021704098No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23770.2424372233441446No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21200.21622503722742384No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC20040.2043938559451686No Hit
CATCAATACCCAGGCAAAGCCCCCAAACTCATCATTTCTGATGTCAGTAATCGGCCCTCAGGGATTTCTAATCG19170.19552046998347716No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19020.19399057585215104No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19010.1938885829100626No Hit
TCTCTGGGCTCCAGGCTGAGGACGAAGGTAATTATTATTGCAGTTCATTA18980.1935826040837974No Hit
GTCCTGGGCGCAGTCTGGCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG18920.19297064643126693No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18600.18970687228443792No Hit
GGGCACAGGGTCCTGGGCGCAGTCTGGCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18160.18521918283254798No Hit
CTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCGCAGTC17990.18348530281704506No Hit
GTCGATCACCATCTCCTGCACTGCTACCACTGTTGACGTTGGTAGTTATAAGTATGTCTCCTGGTATCATCAATA17950.18307733104869142No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17330.17675376863921016No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16970.17308202272402748No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16930.17267405095567387No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16860.171960100361055No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG16440.1676763967933419No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15960.16278073557309836No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15870.16186279909430268No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15810.16125084144177224No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15680.15992493319462292No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGCTACCACTGTTGACGTT15590.15900699671582727No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15590.15900699671582727No Hit
GTTTGGGGGCTTTGCCTGGGTATTGATGATACCAGGAGACATACTTATAA15460.15768108846867795No Hit
GGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCGCAGTCTGGCCTGACTCAGC15130.15431532137976053No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14980.1527854272484344No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14970.15268343430634598No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG14880.15176549782755033No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAT14740.15033759663831261No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14680.14972563898578217No Hit
TCTCAGGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGG14650.14941966015951696No Hit
GTATGTCTCCTGGTATCATCAATACCCAGGCAAAGCCCCCAAACTCATCA14380.14666585072312996No Hit
GTATCAACGCAGAGTACGGGGCTGGCGTCTCAGGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCT14120.14401403422883138No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13890.141668196560798No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13570.13840442241396894No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13160.13422271178834425No Hit
CTGTTGACGTTGGTAGTTATAAGTATGTCTCCTGGTATCATCAATACCCAGGCAAAGCCCCCAAACTCATCATTT13140.1340187259041674No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT13080.13340676825163697No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC13040.13299879648328336No Hit
CTGCTACCACTGTTGACGTTGGTAGTTATAAGTATGTCTCCTGGTATCATCAATACCCAGGCAAAGCCCCCAAA12580.12830712114721662No Hit
GTATTGATGATACCAGGAGACATACTTATAACTACCAACGTCAACAGTGGTAGCAGTGCAGGAGATGGTGATCGA12260.12504334700038758No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11870.12106562225893969No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCAAGTCATGCTG11800.12035167166432083No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11590.11820981988046428No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCAAGTCATG11580.11810782693837588No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11570.11800583399628746No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT11470.1169859045754034No Hit
GCTTGGTCCCTCCGCCGAATATCCAAGTCATGCTGATTGTTAATGAACTGCAATAATAATTACCTTCGTCCTCAG11290.11515003161781205No Hit
GTTATAAGTATGTCTCCTGGTATCATCAATACCCAGGCAAAGCCCCCAAA11180.11402810925483957No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT11160.11382412337066275No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC11120.11341615160230913No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11120.11341615160230913No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11050.11270220100769027No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10920.11137629276054097No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10750.10964241274503805No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10540.10750056096118148No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA10470.10678661036656265No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10450.10658262448238583No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10360.10566468800359013No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCAAGTCATGCTGA10270.10474675152479448No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10160.103624829161822No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10120.10321685739346838No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10100.10301287150929156No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10080.10280888562511474No Hit
GGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAA10050.10250290679884952No Hit
GTCTGGCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGCTACCA10000.10199294208840749No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9990.10189094914631908No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9920.10117699855170023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC252.6893718E-454.6820073
AGGGGGG2850.047.9911570
TCGACGT3250.047.3789629
CGAGACT3300.046.61586823
CTCGACG3350.045.95058428
CCTAACG306.611101E-445.568342
GTGGTAT2150.044.5086141
TATACAC3550.044.2847253
GTATCAA27950.043.4081231
TCCGAGC3650.042.13295713
ACGAGAC3700.041.56995422
CGACGTC3500.041.08484330
AGACTCG3750.041.02824425
CGAGCCC3600.040.81955715
GACGTCA3450.040.72117631
GACTCGA3800.040.49666626
TTATACA3900.040.3104552
CGTATGC2400.040.3101841
TCGTATG2250.039.88720340
CCCACGA3800.039.57250619
Produced by FastQC (version 0.11.5)