FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980460
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC43700.4457091569263407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38270.39032698937233545No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32440.33086510413479386No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCAAGTCATGCTGATTGTTAATGAACTG30110.3071007486281949No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29360.29945127797156434No Hit
CTCCTGCACTGCTACCACTGTTGACGTTGGTAGTTATAAGTATGTCTCCTGGTATCATCAATACCCAGGCAAAGC28550.29118984966240335No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25500.2600820023254391No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23570.24039736450237642No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22870.2332578585561879No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22860.23315586561409948No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22710.23162597148277342No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21340.21765293841666156No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20930.2134712277910369No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20750.2116353548334455No Hit
GTCCTGGGCGCAGTCTGGCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG18670.1904208228790568No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC18370.18736103461640455No Hit
CATCAATACCCAGGCAAAGCCCCCAAACTCATCATTTCTGATGTCAGTAATCGGCCCTCAGGGATTTCTAATCG17740.18093547926483486No Hit
GGGCACAGGGTCCTGGGCGCAGTCTGGCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17670.18022152867021604No Hit
TCTCTGGGCTCCAGGCTGAGGACGAAGGTAATTATTATTGCAGTTCATTA17590.17940558513350877No Hit
GTCGATCACCATCTCCTGCACTGCTACCACTGTTGACGTTGGTAGTTATAAGTATGTCTCCTGGTATCATCAATA17380.1772637333496522No Hit
CTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCGCAGTC17370.1771617404075638No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17130.17471390979744203No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17080.174203945087No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAT16660.16992024151928686No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16140.16461660853068968No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG16080.16400465087815924No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16060.1638006649939824No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15960.16278073557309836No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15900.1621687779205679No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15730.16043489790506496No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15210.15513126491646778No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15190.15492727903229098No Hit
GGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCGCAGTCTGGCCTGACTCAGC14960.1525814413642576No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14790.15084756134875466No Hit
GTATGTCTCCTGGTATCATCAATACCCAGGCAAAGCCCCCAAACTCATCA14590.14880770250698652No Hit
GTTTGGGGGCTTTGCCTGGGTATTGATGATACCAGGAGACATACTTATAA14090.14370805540256615No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13780.14054627419782553No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13610.13881239418232258No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG13610.13881239418232258No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13560.13830242947188054No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13370.13636456357220078No Hit
TCTCAGGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGTTCCTCACCCTCCTCACTCAGGG13300.13565061297758196No Hit
GTATCAACGCAGAGTACGGGGCTGGCGTCTCAGGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCT13240.13503865532505152No Hit
CTGCTACCACTGTTGACGTTGGTAGTTATAAGTATGTCTCCTGGTATCATCAATACCCAGGCAAAGCCCCCAAA13240.13503865532505152No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13180.13442669767252108No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGCTACCACTGTTGACGTT12760.13014299410480795No Hit
CTGTTGACGTTGGTAGTTATAAGTATGTCTCCTGGTATCATCAATACCCAGGCAAAGCCCCCAAACTCATCATTT12570.1282051282051282No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC12540.127899149378863No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12020.12259551639026578No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC11830.12065765049058606No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT11770.12004569283805562No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11670.11902576341717153No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11670.11902576341717153No Hit
GTATTGATGATACCAGGAGACATACTTATAACTACCAACGTCAACAGTGGTAGCAGTGCAGGAGATGGTGATCGA11570.11800583399628746No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11560.11790384105419904No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCAAGTCATG11480.11708789751749178No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11160.11382412337066275No Hit
GTCTGGCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGCTACCA10990.11209024335515982No Hit
GCTTGGTCCCTCCGCCGAATATCCAAGTCATGCTGATTGTTAATGAACTGCAATAATAATTACCTTCGTCCTCAG10860.11076433510801052No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC10670.10882646920833078No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT10620.10831650449788874No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCCAAGTCATGCTG10550.10760255390326989No Hit
GTTATAAGTATGTCTCCTGGTATCATCAATACCCAGGCAAAGCCCCCAAA10420.10627664565612059No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10230.10433877975644086No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10170.10372682210391042No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10120.10321685739346838No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9940.10138098443587705No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9910.10107500560961181No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9840.10036105501499296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG200.007183761352.06582345
GGGTCGA200.00766693151.2156872
GTGGTAT3200.043.775771
GTATCAA30350.038.2754331
CGTTGTC501.5296105E-435.6708863
CGGTACC601.287538E-534.71055245
GCGGTAC601.2967159E-534.6745844
TTAACGG501.9098533E-434.34885435
GACAGGT2300.034.166451
TATCAAC34750.033.505132
ACGGGAC1253.6379788E-1232.7713516
ATCAACG36200.032.151593
TCAACGC36400.031.8762874
CAACGCA37150.031.3246155
TAACGGC553.3275396E-431.24868636
AACGCAG38000.030.7137376
AATGGTA4200.030.38874470
GTAATAC802.9782695E-629.8956471
GCGTTGT604.515261E-429.66248362
GCGGCAT1204.6183236E-429.54461370
Produced by FastQC (version 0.11.5)