FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558066
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT71080.4562066048549933No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC66390.4261051842476506No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC62790.40299961619084174No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG62110.3986352311134445No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA62060.3983143204459888No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAATTGTTGTAGACAGCATATGAGGTGCAGTAATAAT59450.38156278360480234No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG57120.36660834650136775No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55890.358713944081958No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC53900.34594169951722203No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT52200.3350307368237289No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAATTGTTGTAGA50510.3241839562637269No Hit
GATTATTACTGCACCTCATATGCTGTCTACAACAATTGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT48870.3136580863711807No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC47070.3021053023427762No Hit
GCATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA46160.29626472819508287No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG45790.29388998925591087No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC44440.28522540123460755No Hit
GTCTACAACAATTGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGG41370.26552148625282884No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTTATTATAATTTTGTCTCCTGGTACCAACAGTACCCAGGCAAAGC40740.26147801184288727No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAATTGTTGTAGACAGCATATGAGGTG40380.2591674550372064No Hit
CCTCATATGCTGTCTACAACAATTGGGTCTTCGGAACTGGGACCAAGGTC39800.25544489129472053No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCACCTCATAT37180.23862917232004294No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG36420.2337513301747166No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG35780.2296436736312839No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG34720.22284036748122352No Hit
GTTGTAGACAGCATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGC34100.2188610752047731No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33800.216935611200039No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT33210.213148865324062No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32840.21077412638489No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGTAGATACA32190.20660228770796618No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC32180.20653810557447502No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTTATTATAATTTTGTCTCCTGGTACCAACAGTA30490.19569132501447306No Hit
GTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA29680.19049257220169108No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG29550.18965820446630632No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAAACTTGGAGCCAGAGAAG29100.18677000845920522No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT27980.179581609508198No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG27160.17431867456192485No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC26940.17290666762511986No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC25640.16456299027127222No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTTTGGCAACACGG24750.1588507803905611No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC24610.15795223052168522No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT24600.15788804838819406No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA24450.15692531638582705No Hit
GTGTATTACTGTGCGAGGGGAGATGGCTCCAATGCCCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC24250.15564167371600432No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23780.15262511344192095No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC23120.148389092631506No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG23040.1478756355635769No Hit
GTCAGGGAGGCCGTGTTGCCAAACTTGGAGCCAGAGAAGCGATCAGGGAC22970.14742636062913894No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTACTGTTGGTACCAGGAGA22870.14678453929422758No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA22690.14562926089138714No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC22140.14209924354937467No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT21960.14094396514653423No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTTTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG21680.13914686540878243No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACT21650.138954319008309No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA21460.13773485847197744No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21410.13741394780452176No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21290.13664376220262814No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC20820.13362720192854474No Hit
CAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCG20790.13343465552807135No Hit
ACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTT20720.1329853805936334No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC20720.1329853805936334No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG20690.13279283419315996No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT20650.13253610565919544No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT20600.13221519499173975No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA20570.13202264859126636No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20520.13170173792381068No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT19910.1277866277808514No Hit
GGTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC19690.12637462084404638No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC19210.12329387843647188No Hit
CTGTTGGTACCAGGAGACAAAATTATAATAACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA19070.12239532856759597No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG18930.12149677869872008No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18740.12027731816238851No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC18440.11835185415765442No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18140.11642639015292035No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18130.11636220801942922No Hit
GCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAAACTTGGAGCC18090.11610547948546467No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG18000.11552784028404445No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17980.11539947601706217No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT17670.11340982987883698No Hit
TTACTGCACCTCATATGCTGTCTACAACAATTGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC17600.11296055494439902No Hit
GTACTGTTGGTACCAGGAGACAAAATTATAATAACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA16940.10872453413398406No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG16740.10744089146416133No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16610.10660652372877658No Hit
CATATGCTGTCTACAACAATTGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACC16600.10654234159528544No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG16400.1052586989254627No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16270.10442433119007795No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA16070.10314068852025525No Hit
GTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAAACTTGGAG16040.10294814211978182No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC15870.10185704585043254No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC15790.10134358878250345No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15770.10121522451552116No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG15750.10108686024853888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)