FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558066
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT64600.41461658235273735No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC62750.4027428876568772No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC62620.40190851992149246No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG61170.3926021105652777No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC60630.3891362753567564No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA57980.3721280099816054No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAATTGTTGTAGACAGCATATGAGGTGCAGTAATAAT55650.35717357287817075No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG54310.34857316699035856No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT52200.3350307368237289No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50680.32527505253307626No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC47550.30518604475035077No Hit
GATTATTACTGCACCTCATATGCTGTCTACAACAATTGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT46150.29620054606159174No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAATTGTTGTAGA44250.28400594069827595No Hit
GCATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA42050.26988587133022607No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTTATTATAATTTTGTCTCCTGGTACCAACAGTACCCAGGCAAAGC41430.2659065790537756No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC41220.2645587542504618No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCCAATTGTTGTAGACAGCATATGAGGTG40300.2586539979692773No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG39930.2562792590301053No Hit
GTCTACAACAATTGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGG39610.25422543075838894No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG38790.24896249581211582No Hit
GTTGTAGACAGCATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGC35940.23067058776714208No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC35760.22951530936430165No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG35520.2279749381605144No Hit
CCTCATATGCTGTCTACAACAATTGGGTCTTCGGAACTGGGACCAAGGTC35420.227333116825603No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34960.2243807386850108No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCACCTCATAT34880.2238672816170817No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG32220.20679483410843955No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTTATTATAATTTTGTCTCCTGGTACCAACAGTA31490.20210953836358667No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT30220.1939584074102124No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30070.19299567540784535No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG29480.18920892953186835No Hit
GTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCA29080.18664164419222293No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT27600.17714268843553482No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG27580.17701432416855256No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGTAGATACA27410.17592322789920325No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT26730.171558842821806No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAAACTTGGAGCCAGAGAAG26230.1683497361472492No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA25800.1655899044071304No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC25770.16539735800665697No Hit
GTGTATTACTGTGCGAGGGGAGATGGCTCCAATGCCCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC25700.16494808307221903No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC25690.16488390093872787No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC24650.15820895905564977No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT23940.15365202757777913No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTTTGGCAACACGG23460.1505712851702046No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTACTGTTGGTACCAGGAGA22670.14550089662440488No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC22170.1422917899498481No Hit
GTCAGGGAGGCCGTGTTGCCAAACTTGGAGCCAGAGAAGCGATCAGGGAC22120.14197087928239238No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21790.13985286887718493No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC21700.1392752296757647No Hit
GGTACCAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTC21580.13850504407387107No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACT21340.13696467287008382No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21330.1369004907365927No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21320.13683630860310156No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA21270.13651539793564585No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG21210.13613030513469904No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT21150.13574521233375222No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC20950.1344615696639295No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT20420.13105991658889932No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20240.12990463818605885No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTTTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG20130.12919863471765636No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG20110.1290702704506741No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA19790.12701644217895774No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA19700.12643880297753754No Hit
CTGTTGGTACCAGGAGACAAAATTATAATAACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA19420.12464170323978573No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19240.12348642483694529No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC19080.12245951070108711No Hit
CAACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCG19070.12239532856759597No Hit
ACAGTACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAGCGGCCCTCAGGGGTCCCTGATCGCTT18900.12130423229824666No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18750.12034150029587964No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18630.11957131469398599No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT18390.11803094349019876No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC18060.11591293308499126No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC17920.11501438321611537No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC17760.1139874690802572No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA17270.11084254453919153No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17130.10994399467031564No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16970.10891708053445746No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG16860.10821107706605497No Hit
GCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAAACTTGGAGCC16850.10814689493256384No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16490.10583633812688295No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG16440.10551542745942727No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16390.10519451679197157No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT16360.10500197039149818No Hit
GTACTGTTGGTACCAGGAGACAAAATTATAATAACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTGA16330.10480942399102477No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG16100.10333323492072864No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG16050.10301232425327297No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15940.10230632078487047No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC15880.10192122798392367No Hit
GGGTACTGTTGGTACCAGGAGACAAAATTATAATAACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGA15730.10095849598155662No Hit
GTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAAACTTGGAG15730.10095849598155662No Hit
TTACTGCACCTCATATGCTGTCTACAACAATTGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC15720.10089431384806549No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG15710.10083013171457436No Hit
CATATGCTGTCTACAACAATTGGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACC15690.10070176744759209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT801.8735591E-1067.4894470
GCGTCGA350.004735587657.848170
TCCGTAA200.007692795251.17349224
GTACGTA703.2941898E-948.8536569
GTGGTAT3400.048.1122551
GGTATCA12200.045.5324671
TGGTATC3550.045.1048742
GTATCAA29400.036.8615151
TGTTAGA502.0045835E-434.0685772
GGTACGT951.2901E-732.2304968
TATCAAC34600.031.1146552
AATACGT1900.030.5078710
ATGGCAA3550.030.41777870
ATCAACG35200.030.2900453
TCAACGC35700.029.8658124
CAACGCA36350.029.5210825
GTGTTAG704.187977E-529.2110141
AACGCAG37050.028.8787966
TCTGGAG12350.028.41660970
GAATACG2050.028.2728619
Produced by FastQC (version 0.11.5)