FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023186
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTTATTCTGCATTCAATTTCGGCCCCGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT59130.5779007922313245No Hit
CTACTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC50550.4940450709841612No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49840.487105961183988No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTGAAGCTCTTTG36400.3557515446849351No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33050.3230106745010194No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32780.3203718580981366No Hit
GAGTATTAGTCGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATGCCGC31040.30336615239066994No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30420.2973066480581243No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA29890.2921267491932063No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29570.28899926308608603No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29030.2837216302803205No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24420.23866628354961855No Hit
CCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGTCG23610.23074983434097024No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23230.2270359445887649No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23100.22576540335774728No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCGGGGCCGAAATTG22370.21863082567587905No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20890.20416620243044764No Hit
GAGTGAACTCTGTCCCAGATCCACCGCCGCTGAACCTTGATGGGACCCCA20780.20309112908112503No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC20500.20035457873739476No Hit
ATAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGAGTATTAGTCGGTGGTTGGCCTGGTATCAGCAGAAA20160.1970316247485794No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20090.19634748716264688No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20040.1958588174584093No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20030.19576108351756183No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19470.1902879828301013No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT19350.18911517553993118No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19290.1885287718948461No Hit
GTATTAGTCGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT18830.18403301061586064No Hit
GTCCCAGATCCACCGCCGCTGAACCTTGATGGGACCCCACTTTCCAAACT18400.17983045115941773No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC18070.1766052311114499No Hit
CAGTATAGTAGTTATTCTGCATTCAATTTCGGCCCCGGGACCAAAGTGGA17810.17406414864941466No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16410.1603813969307633No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16200.15832898417296562No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16130.15764484658703304No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGA15920.15559243382923535No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15710.15354002107143763No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15670.1531490853080476No Hit
CTAATACTCTGACTGGCCCGACAAGTGATGGTGACT15580.15226947984042002No Hit
GAATAACTACTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAA15460.15109667255024992No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15440.1509012046685549No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT15380.15031480102346984No Hit
ACTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC15190.14845785614736715No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15180.14836012220651962No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC15050.147089580975502No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14850.14513490215855182No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14790.14454849851346677No Hit
GTAGTTATTCTGCATTCAATTTCGGCCCCGGGACCAAAGTGGATATCAAA14710.14376662698668669No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14350.14024820511617633No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14030.137120719009056No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13780.1346773704878683No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13540.13233175590752805No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13280.1297906734454928No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGA13250.12949747162295028No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12960.1266631873383725No Hit
GTCTCCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT12960.1266631873383725No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12770.12480624246226982No Hit
ACTTATTACTGCCAACAGTATAGTAGTTATTCTGCATTCAATTTCGGCCC12660.12373116911294721No Hit
CTTCTCCCTCTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGT12490.12206969211853953No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12490.12206969211853953No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC12420.12138555453260697No Hit
GTCAGAGTATTAGTCGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATG12320.12040821512413187No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATGCCGCCAGTTTGGAAAGTGGGGTCCCAT12210.11933314177480928No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12200.11923540783396176No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12190.11913767389311425No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT12090.11816033448463915No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCGACTA12060.11786713266209663No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11990.11718299507616406No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11950.11679205931277403No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11870.11601018778599395No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11830.1156192520226039No Hit
CTCCTGATCTATGATGCCGCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT11750.11483738049582384No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11720.11454417867328132No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11710.11444644473243379No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGCGGCATCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG11710.11444644473243379No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11510.1124917659154836No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC11500.11239403197463609No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11500.11239403197463609No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11400.11141669256616099No Hit
GGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11210.1095597476900583No Hit
CCCCTAAACTCCTGATCTATGATGCCGCCAGTTTGGAAAGTGGGGTCCCA11180.10926654586751577No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11080.10828920645904068No Hit
CTATAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGAGTATTAGT10970.10721413310971807No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10710.10467305064768281No Hit
CATCAAGGTTCAGCGGCGGTGGATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG10640.10398891306175025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)