FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023186
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTAGTTATTCTGCATTCAATTTCGGCCCCGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT57530.5622633616957229No Hit
CTACTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC49140.4802645853246624No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48840.4773325670992371No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34500.33718209592390824No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34270.3349342152844155No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTGAAGCTCTTTG33980.33209993099983776No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA29090.28430803392540555No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28980.28323296057608294No Hit
GAGTATTAGTCGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATGCCGC28470.27824852959285995No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26780.2617314935896308No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25520.2494170170428446No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23380.22850195370147752No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22630.2211719081379143No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCGGGGCCGAAATTG22220.2171648165631664No Hit
GAGTGAACTCTGTCCCAGATCCACCGCCGCTGAACCTTGATGGGACCCCA22220.2171648165631664No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21410.20924836735451816No Hit
CCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGTCG20760.20289566119943No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20690.20221152361349745No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20690.20221152361349745No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20460.19996364297400473No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18930.18501035002433575No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18710.18286020332569053No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18640.18217606573975798No Hit
GTCCCAGATCCACCGCCGCTGAACCTTGATGGGACCCCACTTTCCAAACT18440.18022138692280779No Hit
ATAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGAGTATTAGTCGGTGGTTGGCCTGGTATCAGCAGAAA18330.17914631357348518No Hit
GTATTAGTCGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT18070.1766052311114499No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17560.17162080012822692No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGA17540.17142533224653192No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT17340.16947065342958173No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17230.16839558008025912No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17230.16839558008025912No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC16750.16370435091957866No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16160.1579380484095756No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16120.15754711264618554No Hit
CAGTATAGTAGTTATTCTGCATTCAATTTCGGCCCCGGGACCAAAGTGGA15510.15158534225448744No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC15300.14953292949668975No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15230.1488487919107572No Hit
GTAGTTATTCTGCATTCAATTTCGGCCCCGGGACCAAAGTGGATATCAAA15130.1478714525022821No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15100.14757825067973956No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14910.14572130580363687No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT14810.1447439663951618No Hit
GAATAACTACTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAA14680.14347342516414416No Hit
ACTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC14430.14103007664295641No Hit
CTAATACTCTGACTGGCCCGACAAGTGATGGTGACT14420.1409323427021089No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14250.13927086570770125No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14190.13868446206261617No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14120.13800032447668362No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14090.13770712265414106No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13440.13135441649905294No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12750.12461077458057479No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC12380.12099461876921692No Hit
GTCTCCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT12280.12001727936074184No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12090.11816033448463915No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11920.11649885749023148No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11920.11649885749023148No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11890.11620565566768895No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11790.11522831625921386No Hit
CTCCTGATCTATGATGCCGCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT11780.11513058231836637No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11760.11493511443667133No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATGCCGCCAGTTTGGAAAGTGGGGTCCCAT11730.1146419126141288No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACCGACTA11710.11444644473243379No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC11690.11425097685073876No Hit
CCCCTAAACTCCTGATCTATGATGCCGCCAGTTTGGAAAGTGGGGTCCCA11590.11327363744226368No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11530.11268723379717861No Hit
ACTTATTACTGCCAACAGTATAGTAGTTATTCTGCATTCAATTTCGGCCC11390.11131895862531349No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11380.11122122468446598No Hit
CTTCTCCCTCTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGT11260.11004841739429587No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11210.1095597476900583No Hit
GTCAGAGTATTAGTCGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATG11190.1093642798083633No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAGAGGGAGAAGTG11120.10868014222243072No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11070.10819147251819317No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11060.10809373857734567No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11040.10789827069565064No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10960.10711639916887057No Hit
GGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10940.10692093128717554No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC10910.10662772946463302No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGCGGCATCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG10880.10633452764209049No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10850.10604132581954796No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT10730.10486851852937785No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10600.10359797729836023No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10460.10222970212649508No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10290.10056822513208742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22750.039.2051661
GTGGTAT2450.038.6111451
TATCAAC25550.034.9052962
CGGAACT601.478286E-534.01754430
ATCAACG26400.033.395943
TCAACGC26400.033.395944
CAACGCA26900.032.7751965
AACGCAG26950.032.7207876
CGAAACC550.004013080631.5647169
ACGCAGA28700.030.8463547
CGGGTAT553.6466288E-430.76454417
AGAGTAC29250.030.28706611
CGCAGAG29350.030.0569048
GCAGAGT30000.029.4085489
GTTCTAA950.007382774327.86197370
CCGTTTA1504.802132E-1027.04823766
TCTCCTA2500.027.0251622
CCCCCGA550.00915184526.66390865
AATGGTA4000.026.46887670
GCCGGGA3600.026.3375421
Produced by FastQC (version 0.11.5)