FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095411
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGTTTACCTCCCACTTTCGGCGGAGGGA45390.41436501915719304No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36070.32928279887640344No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34700.3167760776548711No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGCGAAGTCTGTCCCAGACCCACTGCCA29610.27030950026976175No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27750.25332957218797325No Hit
GTATGGTAGTTTACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT26350.24054898115867013No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25940.23680609378580278No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24910.2274032303856726No Hit
GTGTATTACTGTCAGCAGTATGGTAGTTTACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAA21970.20056398922413599No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21020.19189144531139454No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20890.19070467614438782No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19200.17527667697330043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19150.17482022729368246No Hit
GTCTGGGACAGACTTCGCTCTCACCATCAGCAGACTGGAGCCTGAAGATT18620.16998186068973198No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACA18330.16733445254794777No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18150.16569123370132308No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17830.16276995575176806No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGCGAAGTCTGTC17740.1619483463284557No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGCGAAGTCTGTCCCAGACCCACTGCCA17640.16103544696921976No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17240.15738384953227602No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16650.15199774331278396No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16250.14834614587584022No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16250.14834614587584022No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15620.1425948799126538No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15450.1410429510019527No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT14880.13583942465430784No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14760.1347439454232247No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14710.13428749574360674No Hit
GTGTATTACTGTGCGAAAGGCGGTGGTACCTATGACTACTGGGGCCAGGG14210.12972299894742703No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAACAACTACTTAGCCTGGTACC13910.1269843008697192No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT13650.12461076253570579No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC13630.12442818266385858No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13470.12296754368908108No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAA13210.12059400535506765No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA12850.11730756766181824No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12550.11456886958411043No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12530.11438628971226325No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12210.11146501176270823No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC12020.10973050298015996No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11700.10680922503060494No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11660.10644406528691058No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11360.10370536720920275No Hit
GTCAGAGTGTTACCAACAACTACTTAGCCTGGTACCAGCAGAGACCTGGC11330.10343149740143197No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11190.10215343829850167No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11080.10114924900334213No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA553.52266E-451.7684370
GGTATCA11750.048.0799141
AACTGTG22650.038.02636770
GTATCAA30550.037.759671
TCTGGAG8950.035.78962770
CGAAATT400.002298951135.36605553
TGATCGG400.00232007235.30019452
TCAGCAT7950.034.02390370
CCTAACG601.5488584E-533.7869382
TATCAAC34300.033.294422
ATCAACG34650.033.1334423
TCAACGC34950.032.9366264
CAACGCA35700.032.0526355
AACGCAG36250.031.5663196
CGCAGAG38000.029.9349488
ACGCAGA38300.029.7004747
AGAGTAC38350.029.2136611
GCAGAGT39100.028.9201329
AGTGTCG500.007129434528.0646149
CGGAAAT854.9837745E-627.9831230
Produced by FastQC (version 0.11.5)