FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422125
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT67310.4733057923881515No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49950.35123494770150304No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47700.3354135536608948No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46760.3288037268172629No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40370.2838709677419355No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39040.27451876593126484No Hit
GTAATATATAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAAAAACA37090.260806891096071No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35450.2492748527731388No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35020.24625120857871144No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACGACATAAGCAATTATTTAACCTGGTCTCAGCAAAAA34490.2425243913158126No Hit
GTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCTTGGCCGAAGGTG34370.2416805836336468No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33730.23718027599542937No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32960.231765843368199No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT32710.2300079106970203No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32410.22789839149160587No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30960.21770238199876946No Hit
ATATATTACTGTGCGACCACTATACTTGTATCAACAATTGATTACTGGGGCCAGGGAACCCCGGTCACCGTCTC30640.21545222817966073No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30030.2111628724619847No Hit
TAGTTATCCCATCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAACTGTGGCTGCACCATCTGTCTTCAT29320.20617034367583723No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29280.20588907444844862No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28560.200826228355454No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28210.1983651226158038No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26780.1883097477366617No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC26490.1862705458380944No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT25630.1802232574492397No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25140.17677770941372947No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24710.1737540652193021No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24390.1715039114001934No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24090.16939439219477895No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22600.15891711347455392No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGATGCATAGTTAT22090.15533093082534938No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22040.15497934429111365No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21870.15378395007471213No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT21770.15308077700624065No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21760.15301045969939353No Hit
GATGCATAGTTATCCCATCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAACTGTGGCTGCACCAT21400.1504790366528962No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20730.1457677770941373No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20340.14302540212709852No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT20320.14288476751340423No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20100.141337786762767No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19760.13894699832996396No Hit
CTCCTGATCTCTGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT19180.1348685945328294No Hit
ACTTATTACTGTCAACAGATGCATAGTTATCCCATCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAA19050.13395446954381648No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18820.13233717148633206No Hit
CTGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA18690.13142304649731915No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18450.1297354311329876No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC18410.12945416190559902No Hit
GGATAACTATGCATCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTG18270.12846971960973894No Hit
CTATGCATCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC18110.12734464270018458No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17970.1263602004043245No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17940.1261492484837831No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17740.12474290234684013No Hit
GTCACGACATAAGCAATTATTTAACCTGGTCTCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTCTGCTG17710.12453195042629868No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17660.12418036389206294No Hit
CTCCTGTGCAGCGTCTGGGTTCACCTTCAGCACCTTTGCCATGCACTGGGTCCGCCAGGCTCCAGGCAGGGGG17630.12396941197152149No Hit
GGACACGACTGGAAATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17570.12354750813043859No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17440.12263338314142569No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGAGACCAGGTTAAAT17350.12200052737980135No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17180.12080513316339984No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG17050.11989100817438691No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCC16900.1188362485716797No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16710.1175002197415839No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16670.11721895051419531No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16290.1145468928540037No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16250.1142656236266151No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16130.11342181594444932No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15840.11138261404588204No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15830.11131229673903488No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15790.1110310275116463No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15680.11025753713632769No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG15480.10885119099938473No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCAGAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTG15320.10772611408983036No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15290.10751516216928891No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15150.10653071987342885No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15150.10653071987342885No Hit
TCTCTGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT15100.10617913333919311No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14850.10442120066801441No Hit
GTCGTGTCCCTTGGCCGAAGGTGATGGGATAACTATGCATCTGTTGACAGTAATAAGTTGCAAAATCTTCAGG14810.10413993144062583No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14770.10385866221323722No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14680.1032258064516129No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14640.10294453722422431No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14600.10266326799683573No Hit
CTATACTTGTATCAACAATTGATTACTGGGGCCAGGGAACCCCGGTCACCGTCTCCTCAGCATCCCCGACCAGCC14520.10210072954205854No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14510.10203041223521139No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14300.10055374879142129No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14250.10020216225718555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)