FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130277
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38100.3370855108968863No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31200.27603852860847383No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23770.2103024302892123No Hit
GTAATATACAGCCGTGTCCTCAGGTCTCAGACTGCTCATTTGCAGATACA23170.20499399704674162No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22220.19658897774616313No Hit
CTCCTGTAGGGCCAGTCAGAGTGTCAGCATCTCCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT22210.19650050385878862No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22070.19526186943554544No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20770.18376026407685903No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19600.17340881925404125No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGACT19050.16854275544844316No Hit
GCCTAGAGCCTGAGGACTTTGCAGTTTATTACTGTCAGCAGCATAGCGACTGGTTGCTCAGTTTCGGCGGAGGGA18570.16429600885446666No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTCAGCAT18460.16332279609334702No Hit
GTATATTACTGTGTGAAAGTTCTACAAGGTTACTACTACTACATGGACGT18440.16314584831859802No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17770.15721809786450577No Hit
GTTTATTACTGTCAGCAGCATAGCGACTGGTTGCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA17190.15208661239678414No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16890.14943239577554884No Hit
GCATACAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTC16600.146866653041688No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16280.14403548864570367No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG15780.13961179427697812No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15740.13925789872748007No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15320.13554199545775064No Hit
GTAATAAACTGCAAAGTCCTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15150.13403793937238395No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT15050.13315320049863882No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTAGGGCCAGTCAGAGTGTCAGCATCTCCTTAGCCTGGTA14540.12864103224253878No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAACTG14090.12465970731068579No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14000.12386344232431519No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13750.12165159513995241No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13580.12014753905458574No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13450.11899737851871708No Hit
GTTGTATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTT13390.11846653519447002No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13150.11634316189748177No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAGGACTTTGCAGTTTA12920.11430826248786802No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12900.11413131471311899No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12820.11342352361412292No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12580.11130015031713465No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12470.11032693755601503No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12250.1083805120337758No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12030.10643408651153656No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGC252.8038485E-454.223146
TATGTCG306.8918E-445.185955
GGTATCA12250.045.1536751
GTCACCG7900.039.8988470
GTATCAA35200.035.284181
ACGGGTC1350.032.64874616
TATCAAC37900.032.3042372
TCTGGAG8200.031.60550370
ATCAACG38850.031.5918053
TCAACGC38600.031.2555684
CAACGCA38750.031.2220345
TTATGCG450.004696356630.56762338
AACGCAG40350.029.8999966
CGCAGAG41450.029.2700318
AGAGTAC43250.028.20357911
GCAGAGT43750.027.5763429
TATGCGG500.00777728527.56904239
CTAGAGA3900.026.0688194
AATACGT3800.025.86072310
GTGCTAG3850.025.5610621
Produced by FastQC (version 0.11.5)