FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485231
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC85630.5765433121177783No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTCTGGTCCCACCGCCGAATACCACAGAATAGGTGTCATCATAAGACTGA80470.5418012416923698No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTCACCATCCCCTGCACCCGCAGCAATGGCAACATTGCCAGCAAATATGTG51100.3440542245617012No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49360.33233887523220296No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48000.32318205046891696No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTCTGGTCCCAC41410.2788118481232886No Hit
GTCTGGTCCCACCGCCGAATACCACAGAATAGGTGTCATCATAAGACTGA40950.2757146868062948No Hit
GAATAGGTGTCATCATAAGACTGACAGTAGTAGTCAGCCTCGTCTTCAGG40100.26999167132924107No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT37760.2542365463688813No Hit
GACTACTACTGTCAGTCTTATGATGACACCTATTCTGTGGTATTCGGCGGTGGGACCAGACTGACCGTCCTGGGT37240.2507354074888014No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36900.24844620129797992No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGTTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTCAC36270.24420443688557536No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35700.240366650036257No Hit
TCGCTGGACTGCAGCCTGAAGACGAGGCTGACTACTACTGTCAGTCTTAT35540.23928937653469393No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCTTCAGGCTGCAGTCCAGC34450.2319504508052956No Hit
GTAGTAGTCAGCCTCGTCTTCAGGCTGCAGTCCAGCGATGGTGAGGGAGG34430.23181579161760021No Hit
ACCAAAGACCCTCCGGGGTCCCTCATCGGTTCTCTGGCTCCCTCGACACC33560.22595811695285112No Hit
GCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTCACCATCCCCTGCACCCGCAGCAATGGCAACATTGCCAG32940.22178368213429428No Hit
CACCTATTCTGTGGTATTCGGCGGTGGGACCAGACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA31700.21343481249718058No Hit
GGACCAGACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG31440.21168424305714062No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31110.20946236646016678No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30930.20825043377090835No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG30530.20555725001700073No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30500.20535526123545764No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG30210.2034027030138746No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29380.19781434672451625No Hit
GACCCAGGACGGTCAGTCTGGTCCCACCGCCGAATACCACAGAATAGGTGTCATCATAAGACTGACAGTAGTAGT29240.19687173241064856No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT29040.19552514053369474No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27560.18556036064423648No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27560.18556036064423648No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27510.18522371267499804No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27130.18266518810878576No Hit
GTCCAGCGATGGTGAGGGAGGCAGAGTTGGAGGAGGTGTCGAGGGAGCCA26960.181520585013375No Hit
CAGTAGTAGTCAGCCTCGTCTTCAGGCTGCAGTCCAGCGATGGTGAGGGA26940.18138592582567964No Hit
GCCTGGAGCATCCCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT26560.1788274012594674No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT26250.17674018385018897No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26050.17539359197323515No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25560.17209444187469827No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25500.17169046431161214No Hit
GTCTTATGATGACACCTATTCTGTGGTATTCGGCGGTGGGACCAGACTGACCGTCCTGGGTCAGCCCAAGGCTGC25190.16960324690233372No Hit
ATTTTATGTTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACG24990.16825665502537987No Hit
ACCCAGGACGGTCAGTCTGGTCCCACCGCCGAATACCACAGAATAGGTGT24680.16616943761610148No Hit
GAGTTGGAGGAGGTGTCGAGGGAGCCAGAGAACCGATGAGGGACCCCGGA24450.1646208569576046No Hit
CCCCTGCACCCGCAGCAATGGCAACATTGCCAGCAAATATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCC24170.1627356283298692No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23700.15957113741902773No Hit
CTGTGTCGGAGTCTCCGGGGAAGACGGTCACCATCCCCTGCACCCGCAGC23620.15903250066824623No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23550.1585611935113124No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGT23230.15640664650818625No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCATCACTGTGATCTATGACGACAACCAAAGACCCTCCGGGGTCC23120.15566602097586166No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCATCACTGTGATCTATGACGAC22890.15411744031736477No Hit
TCCCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGC22750.1531748260034971No Hit
GTCTTTGGTTGTCGTCATAGATCACAGTGATGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCACATATTTG22520.15162624534500022No Hit
GAGTTGGGACTGAGCTTGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAATGTGAAGTGCAACTGGTGGAAT22140.14906772077878794No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21650.1457685706802511No Hit
CTACTACTGTCAGTCTTATGATGACACCTATTCTGTGGTATTCGGCGGTG21260.14314271652019112No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21100.14206544301862808No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGTTGACT20650.139035611295482No Hit
CCTCCAACTCTGCCTCCCTCACCATCGCTGGACTGCAGCCTGAAGACGAG20520.138160326575462No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20490.13795833779391892No Hit
GTCACCATCCCCTGCACCCGCAGCAATGGCAACATTGCCAGCAAATATGT20260.13640975713542203No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGTTGACTCAGCCGCACT19960.1343898693199913No Hit
CCACAGAATAGGTGTCATCATAAGACTGACAGTAGTAGTCAGCCTCGTCT19740.1329086182553421No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19450.13095606003375906No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19120.12873418343678525No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19110.12866685384293758No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19040.12819554668600372No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19020.12806088749830835No Hit
GAGCCTGGAGCATCCCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGG18730.1261083292767253No Hit
ATTCTGTGGTATTCGGCGGTGGGACCAGACTGACCGTCCTGGGTCAGCCC18670.12570435171363917No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18620.1253677037444007No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCAT18600.1252330445567053No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT18590.1251657149628576No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18400.1238864526797515No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGTT18250.12287650877203612No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18220.12267451999049304No Hit
GTCTGAAACCGGATGATACGGCCCTGTATTTTTGTGTTAAAGACATGGGT18150.12220321283355923No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17950.1208566209566054No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17830.12004866583043311No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17620.1186347443596316No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTCTGGTC17580.11836542598424084No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17540.11809610760885006No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17470.11762480045191623No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17350.11681684532574395No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17170.11560491263648551No Hit
GCACAGGTTCTTGGGCCAATTTTATGTTGACTCAGCCGCACTCTGTGTCG16850.11345036563335939No Hit
CTCCGACACAGAGTGCGGCTGAGTCAACATAAAATTGGCCCAAGAACCTG16700.11244042172564403No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16570.11156513700562405No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16550.11143047781792867No Hit
GGTTCTTGGGCCAATTTTATGTTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTCACCATCCC16430.11062252269175638No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16010.10779467975015333No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15930.10725604299937182No Hit
GTTTCAGACTGTTCATTTGGAGAGAGAGGGAATTCCTGGAGTTGTCTCTG15890.10698672462398107No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15760.10611143990396107No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15690.10564013274702723No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15650.10537081437163648No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15430.10388956330698726No Hit
GATCTATGACGACAACCAAAGACCCTCCGGGGTCCCTCATCGGTTCTCTGGCTCCCTCGACACCTCCTCCAACT15390.10362024493159651No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15360.10341825615005343No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14930.10052308361460272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCG150.002470547668.17641436
GGCGTTA200.006217205454.0046757
ATTATCG200.00693132852.5402848
GTCGTAT200.007537019451.4388339
GCGGTAC306.125079E-446.28145244
GGTATCA9650.045.756361
TAACGGC306.6967536E-445.45094336
TAGGCCC307.0804544E-444.9398464
CGTATGC2150.043.2472841
CTTATAC2550.042.297751
GACGGAC2350.041.64373826
TCGTATG2350.040.9405540
CGAGACG2500.040.45403723
ACTCCTA2450.040.11844331
ATGCCGT2550.039.47535344
CACGAGA2600.038.8902521
CGGTACC459.5476404E-538.64693545
GTATCAA24250.038.223321
GTATGCC2550.037.92085642
TTATACA2850.037.8440782
Produced by FastQC (version 0.11.5)