FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485231
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC81400.548062893920205No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTCTGGTCCCACCGCCGAATACCACAGAATAGGTGTCATCATAAGACTGA76770.5168892919687241No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTCACCATCCCCTGCACCCGCAGCAATGGCAACATTGCCAGCAAATATGTG53100.3575201433312394No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG52610.3542209932327025No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT44370.2987414079022051No Hit
GTCTGGTCCCACCGCCGAATACCACAGAATAGGTGTCATCATAAGACTGA40870.2751760500555132No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTCTGGTCCCAC38630.26009422103363045No Hit
GAATAGGTGTCATCATAAGACTGACAGTAGTAGTCAGCCTCGTCTTCAGG38020.2559871158089213No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37940.2554484790581398No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGTTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTCAC36090.24299250419631693No Hit
GACTACTACTGTCAGTCTTATGATGACACCTATTCTGTGGTATTCGGCGGTGGGACCAGACTGACCGTCCTGGGT35640.23996267247317085No Hit
CACCTATTCTGTGGTATTCGGCGGTGGGACCAGACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCA34440.2318831212114479No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34410.23168113242990485No Hit
GTAGTAGTCAGCCTCGTCTTCAGGCTGCAGTCCAGCGATGGTGAGGGAGG34140.22986323339601716No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33730.22710272004826185No Hit
TCGCTGGACTGCAGCCTGAAGACGAGGCTGACTACTACTGTCAGTCTTAT32910.2215816933527512No Hit
GCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTCACCATCCCCTGCACCCGCAGCAATGGCAACATTGCCAG32890.22144703416505582No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC32870.22131237497736042No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32470.2186191912234528No Hit
ACCAAAGACCCTCCGGGGTCCCTCATCGGTTCTCTGGCTCCCTCGACACC32300.21747458812804202No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCTTCAGGCTGCAGTCCAGC31830.21431009721720054No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG31530.21229020940176985No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30910.20811577458321298No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG29660.1996995753522516No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29570.19909360900762238No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29250.19693906200449626No Hit
GGACCAGACTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG28280.19040809140127024No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCATCACTGTGATCTATGACGACAACCAAAGACCCTCCGGGGTCC27530.1853583718626934No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27490.18508905348730265No Hit
GTCCAGCGATGGTGAGGGAGGCAGAGTTGGAGGAGGTGTCGAGGGAGCCA27360.18421376876728265No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27320.1839444503918919No Hit
GACCCAGGACGGTCAGTCTGGTCCCACCGCCGAATACCACAGAATAGGTGTCATCATAAGACTGACAGTAGTAGT27130.18266518810878576No Hit
GTCTTATGATGACACCTATTCTGTGGTATTCGGCGGTGGGACCAGACTGACCGTCCTGGGTCAGCCCAAGGCTGC26970.1815879146072227No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26450.1780867757271428No Hit
GTCTTTGGTTGTCGTCATAGATCACAGTGATGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCACATATTTG25940.17465296644091052No Hit
CAGTAGTAGTCAGCCTCGTCTTCAGGCTGCAGTCCAGCGATGGTGAGGGA25850.17404700009628132No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG25580.17222910106239364No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCATCACTGTGATCTATGACGAC25310.17041120202850601No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25110.16906461015155216No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24680.16616943761610148No Hit
GAGTTGGAGGAGGTGTCGAGGGAGCCAGAGAACCGATGAGGGACCCCGGA24580.16549614167762455No Hit
ATTTTATGTTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACG24130.16246630995447847No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23690.15950380782518006No Hit
CTGTGTCGGAGTCTCCGGGGAAGACGGTCACCATCCCCTGCACCCGCAGC23670.1593691486374847No Hit
GCCTGGAGCATCCCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT23330.15707994244666318No Hit
GAGTTGGGACTGAGCTTGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAATGTGAAGTGCAACTGGTGGAAT22740.1531074964096494No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22600.15216488209578174No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGT22410.15088561981267562No Hit
CCCCTGCACCCGCAGCAATGGCAACATTGCCAGCAAATATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCC22300.150144994280351No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21650.1457685706802511No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21430.14428731961560187No Hit
GTCACCATCCCCTGCACCCGCAGCAATGGCAACATTGCCAGCAAATATGT21160.14246942058171422No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21080.1419307838309327No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21040.14166146545554192No Hit
ACCCAGGACGGTCAGTCTGGTCCCACCGCCGAATACCACAGAATAGGTGT20880.1405841919539789No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20670.13917027048317734No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20370.13715038266774662No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20290.13661174591696512No Hit
CTACTACTGTCAGTCTTATGATGACACCTATTCTGTGGTATTCGGCGGTG20100.13533248363385897No Hit
ATTCTGTGGTATTCGGCGGTGGGACCAGACTGACCGTCCTGGGTCAGCCC20010.13472651728922974No Hit
CCTCCAACTCTGCCTCCCTCACCATCGCTGGACTGCAGCCTGAAGACGAG19850.13364924378766668No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19320.13008077531373907No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18860.1269836139967453No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18720.1260409996828776No Hit
GTCTGAAACCGGATGATACGGCCCTGTATTTTTGTGTTAAAGACATGGGT18690.12583901090133454No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18690.12583901090133454No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18570.12503105577516224No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT18560.12496372618131457No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18560.12496372618131457No Hit
GGTTCTTGGGCCAATTTTATGTTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTCACCATCCC18440.12415577105514228No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT18410.1239537822735992No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18360.12361713430436073No Hit
TCCCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGC18220.12267451999049304No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17820.11998133623658543No Hit
CTCCGACACAGAGTGCGGCTGAGTCAACATAAAATTGGCCCAAGAACCTG17780.11971201786119465No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTCTGGTC17730.11937536989195621No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17640.11876940354732697No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGTTGACT17550.11816343720269776No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17520.1179614484211547No Hit
CCACAGAATAGGTGTCATCATAAGACTGACAGTAGTAGTCAGCCTCGTCT17310.11654752695035317No Hit
CTCCAGGCCCTTCCCTGGAGCTTGCCGGACCCAGTGCATGGCATAATCAT16990.11439297994722707No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16780.11297905847642556No Hit
GCTGGTACCACTGCACATATTTGCTGGCAATGTTGCCATTGCTGCGGGTG16680.11230576253794865No Hit
GTTCTCTGGCTCCCTCGACACCTCCTCCAACTCTGCCTCCCTCACCATCGCTGGACTGCAGCCTGAAGACGAGG16440.11068985228560406No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGTTGACTCAGCCGCACT16360.11015121553482252No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16300.10974723797173638No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16290.10967990837788869No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16230.10927593081480255No Hit
GCACAGGTTCTTGGGCCAATTTTATGTTGACTCAGCCGCACTCTGTGTCG16200.10907394203325947No Hit
GTTTCAGACTGTTCATTTGGAGAGAGAGGGAATTCCTGGAGTTGTCTCTG16060.1081313277193918No Hit
GAGCCTGGAGCATCCCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGG16040.10799666853169641No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15780.10624609909165644No Hit
GTCCTGAGATTCCCAGGTGTTTCCATTCAGTGATGAGCACTGAACACAGA15520.10449552965161649No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15480.10422621127622572No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15430.10388956330698726No Hit
GATCTATGACGACAACCAAAGACCCTCCGGGGTCCCTCATCGGTTCTCTGGCTCCCTCGACACCTCCTCCAACT14970.10079240198999347No Hit
GTGATGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCACATATTTGCTGGCAATGTTGCCATTGCTGCGGGTG14950.1006577428022981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTC200.00629709153.83064356
AAACGTC200.0078531150.9070532
GCGACTT200.00807350950.55185321
CGCGCTT200.008083194550.5365218
GGTATCA10500.044.8926051
TGGTATC3350.043.2073252
AATGGTA3550.041.5833270
GTATCAA24050.035.279431
CGGTCAA400.002915938633.69328310
ACAGCTA553.7576878E-430.6085971
TATCAAC28550.029.3580362
ATCAACG29350.028.7862263
TCAACGC29750.028.5123274
CAACGCA30050.028.1175585
AACGCAG30150.027.8075526
ACGCTTA500.00788899427.48914540
AGTTAAC500.00861662626.99647727
CAGATTA500.00866353626.96644224
TCTAAGC500.00872353126.928313
ACGCAGA31200.026.878067
Produced by FastQC (version 0.11.5)