FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451937
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC70050.48245894966517144No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG58890.40559611057504563No Hit
AATTATTACTGTGCATCATGGGATGACAGCCTGAATGGTGTGCTATTCGG54510.3754295124375231No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATAGCAC52980.3648918651429091No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50190.34567615537037766No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC47200.3250829753632561No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATAGCACACCATTCAGG43230.29774019120664325No Hit
GTAATAATTAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG42820.2949163772257336No Hit
GTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAGCGTCTGGGACCC42520.29285017187384854No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41940.28885550819353734No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC36790.25338564965284305No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36750.25311015560592504No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT36110.24870225085523684No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35950.24760027466756476No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG34620.2384400976075408No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCACCCTCAGCGTCT33700.2321037345284265No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG33570.23120837887594298No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32490.2237700396091566No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31040.2137833804083786No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30960.21323239231454258No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29680.20441658281316616No Hit
CCATTCAGGCTGTCATCCCATGATGCACAGTAATAATTAGCCTCATCCTC29650.20420996227797764No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28170.19401668254201113No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27900.19215709772531453No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27740.19105512153764248No Hit
GTTCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC27520.18953990427959339No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27470.18919553672094588No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC27460.18912666320921637No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27410.18878229565056887No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26690.18382340280604462No Hit
GTCTGAGGATGAGGCTAATTATTACTGTGCATCATGGGATGACAGCCTGAATGGTGTGCTATTCGGCGGAGGGAC26350.1814817034072415No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC25590.17624731651579925No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25010.17225265283548805No Hit
GTAATAATCACCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG24450.16839573617863585No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23910.16467656654524265No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGCCAC23780.1637812108927591No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATAGCACACCATTCAGGC23590.16247261416989855No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC23530.16205937309952154No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22910.15778921537229232No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC22830.1572382272784563No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC22020.1516594728283665No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21160.14573635081962924No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20470.1409840785102935No Hit
GATTATTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG20230.1393311142287854No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20200.1391244936935969No Hit
TTATTACTGTGCATCATGGGATGACAGCCTGAATGGTGTGCTATTCGGCG20150.1387801261349494No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20100.13843575857630186No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19180.13209939549718755No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT18980.1307219252625975No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG18970.13065305175086797No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATAGCACAC18910.13023981068049095No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA18800.1294822020514664No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18740.12906896098108941No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18650.12844909937552387No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18430.12693388211747478No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18270.12583190592980273No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT18180.12521204432423721No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18080.12452330920694218No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17880.1231458389723521No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17870.1230769654606226No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17670.12169949522603254No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT17510.1205975190383605No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC17110.11784257856918033No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG16940.11667172886977879No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16810.11577637321729525No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16700.1150187645882707No Hit
CCCCTGAGGGCCGGTGATTATTACTATACATGAGGAGTTTGGGGGCCGTT16580.11419228244751665No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16580.11419228244751665No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC16400.1129525592363856No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16220.11171283602525454No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTAATTATTACTGTGCAT16170.11136846846660702No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC15980.11005987174374646No Hit
TTACTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA15840.1090956425795334No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG15830.10902676906780391No Hit
GTTTATAGTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA15710.10820028692704986No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT15690.10806253990359087No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15650.10778704585667286No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15600.10744267829802531No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC15570.10723605776283682No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15540.10702943722764831No Hit
GGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG15450.10640957562208278No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15330.10558309348132873No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15290.10530759943441072No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15230.10489435836403371No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG15210.1047566113405747No Hit
GCCTGAATGGTGTGCTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA15150.10434337027019767No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15140.10427449675846816No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15100.10399900271155016No Hit
GTATAGTAATAATCACCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC14800.10193279735966505No Hit
GTTTGGTCCCTCCGCCGAATAGCACACCATTCAGGCTGTCATCCCATGATGCACAGTAATAATTAGCCTCATCCT14750.10158842980101754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAACG150.002555886367.594921
CCGGATC200.00798408750.6944413
GTGGTAT3350.046.408451
GGTATCA13350.045.8227731
TCGTATG901.8189894E-1242.09817540
TCTCGTA903.6379788E-1241.92893638
TGGTATC3700.041.1064342
GTATCAA33200.038.582041
CGTATGC1100.037.6677841
ATCTCGT1103.092282E-1134.23488637
ACGAGAC1253.6379788E-1232.4500322
TATCAAC40300.031.5341992
CGAGCCC1307.2759576E-1231.19657915
ATCAACG41350.030.815193
TCAACGC42150.030.1501354
CCGGTTC952.475972E-729.92121554
TAGGCAA1603.0105712E-629.85242370
CAACGCA42700.029.8409375
AACGCAG43500.029.292146
AGACGGA1401.8189894E-1128.99021725
Produced by FastQC (version 0.11.5)