FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126663
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGTAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGG45900.4073977755548909No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37130.3295572855414618No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC32320.28686483890923903No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30230.2683144826802691No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28740.25508958756966366No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28200.25029667256313554No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA27420.24337357310926158No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22370.198550942029693No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21900.1943793308203074No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21710.19269293479949196No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21630.19198287331704333No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21010.1864798968280666No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20830.1848822584925572No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT20790.18452722775133293No Hit
GTGTAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACC20650.18328462015704786No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20570.18257455867459924No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20130.17866922052113188No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20030.17778164366807112No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19510.17316624403215514No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCAC19490.17298872866154297No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG19110.1696159366199121No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19100.169527178934606No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18990.16855084439623916No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18730.16624314457828118No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18540.16455674855746572No Hit
GTAATACATAGCCGTGTCCTCGGCTCTGAGGCTGTTCATTTGCAGATACA18350.16287035253665028No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18040.1601188642921619No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17460.15497091854440945No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17000.15088806502032995No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG16970.15062179196441172No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16630.1476040306640051No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG16280.14449751167829245No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16110.14298863102808915No Hit
CCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAAGTTTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT16020.14218981186033447No Hit
ATGTATTACTGTGCGCTTCAGTGGGATATTGGAGTGCCTAGGGACTACTGGGGCCAGGGAACCCTGGTCACCGT15730.13961583898645824No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15600.13846198907747923No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15410.13677559305666379No Hit
GCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAAGTTTGGCACCTCAG15410.13677559305666379No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15210.13500043935054226No Hit
CCCCTGAGGGCCGCTTATTATTCTTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATA14820.13153888962360527No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC14790.13127261656768707No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14740.13082882814115668No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14580.12940870517625944No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14510.12878740137911693No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCTGAGGCTGTTCATTTGCAGATA14510.12878740137911693No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCCGAAGTTATTTTGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCC14300.1269234899876893No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGTAG14250.12647970156115892No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13930.12363945563136447No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCACCATCTCTTGTTCTGG13780.12230809035177334No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT13560.12035542127503965No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13440.11929032905136673No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13360.11858026756891811No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC13120.11645008312157229No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC12860.1141423833036143No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12840.11396486793300216No Hit
TTATTACTGTGTAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCG12500.11094710663259555No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12340.10952698366769832No Hit
GGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC12270.1089056798705558No Hit
GAATAATAAGCGGCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAAGTTTGGCACCTCAGCCTCCCTGGCCAT12260.10881692218524973No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAAACTTGGAGCCAGAGAATCGCTC12160.10792934533218894No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATACAAAATAACTT12050.1069530107938221No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC11730.10411276486402767No Hit
GCTGGTACCAGTATACAAAATAACTTCGGATGTTGGAGCTGCTTCCAGAA11720.1040240071787216No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGTAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGAC11280.10011866902525422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC200.00795714150.7370723
GGTATCA10000.042.341041
GTGGTAT3500.038.7118071
CTTTCGG350.001485648938.65509818
TGGTATC3400.037.829282
TCTGGAG9100.036.27370570
GTATCAA23250.035.839641
CTAAGTC502.0927985E-433.8187074
TATCAAC26450.030.9679952
ATCAACG28100.029.2505533
TCAACGC28000.029.1082464
CAACGCA28450.028.6478355
AACGCAG29050.028.288976
TCTAGGT500.00851792327.0597723
AGAGTAC30950.026.9870111
CGCAGAG30600.026.8560338
ACGCAGA30800.026.7914457
GACAGGT1800.026.3455371
CAGAGTA31450.026.23539510
GCAGAGT31500.026.196089
Produced by FastQC (version 0.11.5)