FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327660
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATGATACCTCAATCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGG68150.5133091303496377No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48720.36696142084569844No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTTTCCCAGACCCACTGGCA46290.3486585420966211No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44620.3360800204871729No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32860.24750312580028017No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32340.243586460388955No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32210.24260729403612372No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGTTACTTAGTCTGGTACC32110.2418540891493304No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29910.22528358163987766No Hit
CTTCTATAGTACATCCACCAGGGCCACTGACATCCCAGACAGGTTCAGTGCCAGTGGGTCTGGGAAAGACTTCA29690.22362653088893242No Hit
GTGTATTACTGTCAGCACTATGATACCTCAATCACTTTCGGCGGAGGGAC28760.21662172544175468No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27080.20396788334362714No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26340.1983941671813567No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25830.19455282225871082No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25510.19214256662097223No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG25200.18980763147191299No Hit
CTATGATACCTCAATCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT23800.17926276305680672No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23710.17858487865869274No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTTTCCCAGACCCACTGGCA23510.17707846888510614No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22930.17270988054170494No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22340.16826597170962446No Hit
CTATAGAAGAGGAGCCTGGGAGCCTGGCCAGATTTCTTCTGGTACCAGACTAAGTAACTGCTGCTAACACTCTGA22190.1671361643794345No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21920.16510251118509256No Hit
ATGATACCTCAATCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGA21760.16389738336622328No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21690.16337013994546795No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21640.16299353750207132No Hit
CAGTAATACACGGCAGTGTCCTCAGCCGTCAGGCTACTGATCTGAAGATA20330.153126553485079No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20180.15199674615488906No Hit
GTAATACACGGCAGTGTCCTCAGCCGTCAGGCTACTGATCTGAAGATATG19410.1461970685265806No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGA19210.14469065875299397No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18200.1370832893963816No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17850.13444707229260502No Hit
GTATTACTGTGCGCGACCCTTGAGTTACGATGTTTTGCCTGGTTATACTCGATTTTACTACCACTACTACGGTTT17490.13173553470014915No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC17420.13120829127939382No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17140.12909931759637255No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG16950.1276682283114653No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16200.12201919166051549No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16110.12134130726240151No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15790.11893105162466294No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15690.11817784673786964No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15640.11780124429447299No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15640.11780124429447299No Hit
CTATAGTACATCCACCAGGGCCACTGACATCCCAGACAGGTTCAGTGCCAGTGGGTCTGGGAAAGACTTCACTCT15240.11478842474729976No Hit
GTATCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTTTC15120.11388457888314779No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14990.1129054125303165No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14770.11124836177937122No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14570.1097419520057846No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14560.10966663151710529No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14500.1092147085850293No Hit
GTGTATTACTGTGCGCGACCCTTGAGTTACGATGTTTTGCCTGGTTATAC14460.10891342663031198No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14080.10605124806049743No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13950.1050720817076661No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGCGAAATTGTGTTGACGCAGTC13800.10394227437747615No Hit
CTGCAGGAGAGGGCGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGG13690.10311374900200353No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGCGAAATTGTGTTGACGCAGTCTC13360.10062817287558562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1200.057.57003441
TCGTATG1500.048.26225740
GCGAATC1650.047.48079333
ATCTCGT1400.046.49203537
CACGAGA2150.040.8952421
GGCGAAT2150.039.56529632
CGAATCT1850.038.71085434
GTACGGC350.001488416538.640973
CCCACGA2350.037.41338319
GTATGCC2050.037.1409442
TATACAC2700.036.3153533
GTATCAA29050.036.196811
AATCTCG1800.036.0984836
TAAGGCG2350.036.09269729
ACTAAGG2650.034.52290327
CGAGACT2650.034.46436723
AATGGTA7500.034.24994770
ATCTCCG2700.033.81084410
GAGACTA2650.033.1941624
TCTCCGA2700.032.55859411
Produced by FastQC (version 0.11.5)