FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327660
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATGATACCTCAATCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGG65560.4938011237816911No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49160.370275522347589No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTTTCCCAGACCCACTGGCA41760.3145383607248844No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35830.26987331093804134No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34070.2566169049304792No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33310.2508925477908501No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGTTACTTAGTCTGGTACC31560.23771146227196724No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31320.23590377054366327No Hit
CTTCTATAGTACATCCACCAGGGCCACTGACATCCCAGACAGGTTCAGTGCCAGTGGGTCTGGGAAAGACTTCA29810.22453037675308438No Hit
GTGTATTACTGTCAGCACTATGATACCTCAATCACTTTCGGCGGAGGGAC29130.2194085835228899No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27540.20743262582287636No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26700.20110570477381257No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26120.1967371164304114No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG25240.1901089134266303No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23810.17933808354548605No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23630.1779823147492581No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23160.17444225178132955No Hit
CTATGATACCTCAATCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT21960.1654037931398099No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTTTCCCAGACCCACTGGCA21700.1634454604341473No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20590.1550848861907416No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20560.1548589247247036No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20480.15425636081526897No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20340.15320187397375834No Hit
ATGATACCTCAATCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGA20250.15252398957564436No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20210.15222270762092704No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20040.15094225931337843No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCGCCCTCTCCTGCAGGGCCAGTCAGA19840.1494358495397918No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19760.14883328563035716No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19490.14679963243601524No Hit
CTATAGAAGAGGAGCCTGGGAGCCTGGCCAGATTTCTTCTGGTACCAGACTAAGTAACTGCTGCTAACACTCTGA18840.14190380067185876No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18480.13919226307940286No Hit
CAGTAATACACGGCAGTGTCCTCAGCCGTCAGGCTACTGATCTGAAGATA18430.1388156606360062No Hit
GTAATACACGGCAGTGTCCTCAGCCGTCAGGCTACTGATCTGAAGATATG18240.13738457135109894No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17260.13000316346052454No Hit
GTATTACTGTGCGCGACCCTTGAGTTACGATGTTTTGCCTGGTTATACTCGATTTTACTACCACTACTACGGTTT17080.1286473946642966No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC16560.1247307292529714No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG16170.1217932301944775No Hit
GTATCATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGAAGTCTTTC15860.11945829504541826No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15800.11900637211334227No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15510.11682207794164168No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15420.1161441935435277No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14880.11207688715484385No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14790.1113990027567299No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14660.11041983640389859No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14640.11026919542653991No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG14600.10996791347182261No Hit
GTGTATTACTGTGCGCGACCCTTGAGTTACGATGTTTTGCCTGGTTATAC14430.10868746516427398No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14420.10861214467559464No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14100.10620188903785607No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14010.10552400463974211No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGCGAAATTGTGTTGACGCAGTC13900.10469547926426946No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13860.10439419730955216No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13510.10175798020577558No Hit
CTATAGTACATCCACCAGGGCCACTGACATCCCAGACAGGTTCAGTGCCAGTGGGTCTGGGAAAGACTTCACTCT13310.10025157043218896No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT200.00799469150.67717713
GTATCAA25650.039.0882841
TATCAAC28700.034.812742
TACGTAT1002.0548642E-433.9216170
TCAACGC30200.032.9706234
CAACGCA30550.032.704615
ATCAACG30550.032.5928883
AACGCAG31550.031.7797836
ACGCAGA33000.030.3868357
CGCAGAG33700.029.6610538
AGAGTAC33900.029.3000811
GCAGAGT34700.028.812789
TGTTAGA13650.027.336170
GCTAAGT500.00856767127.0278311
GATTCAC2500.027.0064491
AATGGTA6950.025.380270
CAGAGTA39400.025.20711710
GAGTACG30650.024.47054512
AGTACGG31150.024.1862213
GTATTAG1551.9626896E-923.9573331
Produced by FastQC (version 0.11.5)