FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132552
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGTTATTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT68790.6073893295848667No Hit
CCATTATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC52550.4639963551342455No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46590.4113718398801997No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46170.4076634008857871No Hit
CCATAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC39560.3492996348070552No Hit
GATCTATGGTGCCTCCAGTTTGAAAAGTGGGGCCCCATCAAGGTTCAGCGGCAGTGGATTTGGGACAGAATTCA39480.34859326547478614No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38290.33808602165728374No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36640.32351715417923416No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA33300.2940262345570005No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31200.2754840395849374No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30160.26630123826543944No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29490.26038539510768605No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28900.2551759212822016No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28840.2546461442829998No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28690.2533217017849953No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28000.24722926629417458No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27400.24193149630215655No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25720.22709774032450605No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25680.2267445556583715No Hit
GACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT23940.2113810226815193No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAATAACTGGTTGGCCTGGTATCAGCAGAAA23710.2093502108512457No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG23260.20537688335723214No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23150.20440562552536218No Hit
GTAATAAACACCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGAAAAA22760.2009620750305505No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22320.1970770437030706No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21820.1926622353763889No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA21450.18939527721464444No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21190.18709957688476997No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC21160.18683468838516906No Hit
ACTTATTACTGTCAACAGTATAATGGTTATTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCA20190.1782699602314066No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20030.17685722156686845No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19940.17606255606806576No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19690.1738551519047249No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAATAACCATTATA19580.17288389407285495No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAA19180.16935204741150958No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAATAA19100.16864567807924052No Hit
GAGTGAATTCTGTCCCAAATCCACTGCCGCTGAACCTTGATGGGGCCCCA19050.16820419724657235No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18280.16140539242348254No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18250.16114050392388163No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAAATCCACTGCCG17880.1578735457621372No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17710.1563725109310654No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17650.15584273393186363No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17470.1542534029342582No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17340.15310555276932095No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17290.1526640719366528No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAACCAGATGACCCAGTCTCCTTCCACC17170.1516045179382492No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17150.15142792560518192No Hit
GCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTATTA16710.14754289427770206No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16250.14348127061715488No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15790.13941964695660775No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT15590.13765372362593506No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGTTGTCACATTTGGCACCTGGGAGCCAGAG15570.1374771312928678No Hit
ATTAATAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAG15530.13712394662673325No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15460.13650587346099782No Hit
CCCCTAGGCTCCTGATCTATGGTGCCTCCAGTTTGAAAAGTGGGGCCCCA15290.13500483862992604No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15290.13500483862992604No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAATAA15280.1349165424633924No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15210.13429846929765699No Hit
GTTATTAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA15070.13306232296618611No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTATGGTGCCTCCAGTTTGAAAAGTGGGGCCCCAT14900.1315612881351143No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14710.12988366097097528No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14610.12900069930563896No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14580.12873581080603805No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14340.12661670280923082No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14170.12511566797815907No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14040.12396781781322183No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14010.12370292931362092No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13910.12281996764828458No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13900.12273167148175095No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13670.12070085965147737No Hit
GATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC13640.12043597115187646No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13630.12034767498534285No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGTCAAC13410.1184051593216029No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13160.11619775515826206No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13090.1155796819925266No Hit
ACCTTGATGGGGCCCCACTTTTCAAACTGGAGGCACCATAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTG13040.11513820115985843No Hit
CATCAAGGTTCAGCGGCAGTGGATTTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG12910.1139903509949212No Hit
GAATATTAATAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTATGGTGCCTC12890.11381375866185393No Hit
GGGTGGAAGGAGACTGGGTCATCTGGTTGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG12450.10992872733437405No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12360.10913406183557135No Hit
ACCTTGGTCCCTCCGCCGAAATAACCATTATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTG12260.10825110017023501No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12200.10772132317103322No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12170.10745643467143232No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12150.10727984233836504No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12140.10719154617183141No Hit
GTTTATTACTGTGCGAGAAATGAGGCCTGGGGCCAGGGAACCCTGGTCAGCGTCTCCTCAGCATCCCCGACCAGC12100.10683836150569687No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12060.10648517683956232No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12050.10639688067302869No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11960.10560221517422601No Hit
TTAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA11850.10463095734235602No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT11580.10224696084594792No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11530.10180548001327974No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11450.10109911068101067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2150.049.098161
CGAGACG1950.043.69299723
TCGTATG1750.043.34209440
TTAAGCG404.8727787E-543.31658644
CGTATGC1950.042.49170741
ACGAGAC2050.041.55245222
TCTGGAG8600.039.9338370
CACGAGA2150.039.61277821
ATCTCGT1950.038.75813337
CTTATAC2200.038.6953931
ATGCCGT2150.038.6827244
TATGCCG2150.038.63931343
TAAGCGG459.6955846E-538.545245
AATGGTA3450.037.82717570
GTATCAA22250.037.0362741
ACGGAGC2450.036.2135127
CCCACGA2450.036.1415519
GCTACAT2200.035.75447532
CCGAGCC2500.035.40933614
TATACAC2650.034.6944353
Produced by FastQC (version 0.11.5)