FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132552
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGGTTATTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT62170.5489372673396011No Hit
CCATTATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC47340.4179940523702223No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45580.40245392706030275No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40150.35450910863253965No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37700.3328765478317993No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37520.33128721683419393No Hit
CCATAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC33860.29897081988288393No Hit
GATCTATGGTGCCTCCAGTTTGAAAAGTGGGGCCCCATCAAGGTTCAGCGGCAGTGGATTTGGGACAGAATTCA33610.2967634157195431No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA30960.2733649315881302No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28930.2554408097818025No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28130.2483771164591118No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28080.24793563562644366No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27810.24555163913003555No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27060.238929426640013No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26810.23672202247667215No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26520.23416143364719677No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25700.22692114799143878No Hit
GAGTGAATTCTGTCCCAAATCCACTGCCGCTGAACCTTGATGGGGCCCCA24600.21720856967273908No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22950.20263970219468955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22040.19460475104012886No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21960.19389838170785978No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21820.1926622353763889No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21710.19169097754451891No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAATAACTGGTTGGCCTGGTATCAGCAGAAA20920.18471558038836186No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA20750.18321454555729008No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20740.18312624939075645No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG20190.1782699602314066No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19850.17526789056926304No Hit
GACCAAGGTGGAGCTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT19790.17473811357006125No Hit
GTAATAAACACCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGAAAAA19300.17041160140991318No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19200.16952863974457685No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19170.16926375124497595No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19130.16891056657884143No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19080.16846908574617325No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAATAA18700.16511383141789515No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18180.1605224307581462No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGCTCCACCTTGGTCCCTCCGCCGAAATAACCATTATA17830.157432064929469No Hit
ACTTATTACTGTCAACAGTATAATGGTTATTTCGGCGGAGGGACCAAGGTGGAGCTCAAACGAACTGTGGCTGCA17730.15654910326413268No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17540.15487147609999363No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17500.15451829143385912No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAAATCCACTGCCG17330.1530172566027873No Hit
GGGTGGAAGGAGACTGGGTCATCTGGTTGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG16970.1498385946075765No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAA16910.14930881760837472No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16870.14895563294224018No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16660.14710141344503389No Hit
GCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTATTA16490.1456003786139621No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16250.14348127061715488No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15920.14056749712154495No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15850.13994942395580953No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15740.13897816612393957No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15700.13862498145780502No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15180.13403358079805608No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15140.13368039613192154No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAACCAGATGACCCAGTCTCCTTCCACC15020.13262084213351794No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14600.12891240313910532No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14440.12749966447456718No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14430.12741136830803354No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGTTGTCACATTTGGCACCTGGGAGCCAGAG14300.1262635181430963No Hit
ATTAATAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAG14260.12591033347696176No Hit
GTTATTAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA14220.12555714881082725No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14180.1252039641446927No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13800.12184870981641462No Hit
CCCCTAGGCTCCTGATCTATGGTGCCTCCAGTTTGAAAAGTGGGGCCCCA13720.12114234048414554No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAATAA13610.12017108265227558No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13480.11902323248733833No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13110.11575627432559388No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13110.11575627432559388No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12840.11337227782918576No Hit
ACCTTGATGGGGCCCCACTTTTCAAACTGGAGGCACCATAGATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTG12820.11319568549611848No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12450.10992872733437405No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTATGGTGCCTCCAGTTTGAAAAGTGGGGCCCCAT12420.10966383883477314No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12330.10886917333597045No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12270.10833939633676865No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGTCAAC12100.10683836150569687No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12090.10675006533916323No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12050.10639688067302869No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11970.10569051134075964No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11960.10560221517422601No Hit
GAATATTAATAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGGCTCCTGATCTATGGTGCCTC11830.10445436500928874No Hit
GATCAGGAGCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC11650.10286503401168334No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11610.1025118493455488No Hit
ACCTTGGTCCCTCCGCCGAAATAACCATTATACTGTTGACAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTG11530.10180548001327974No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11440.10101081451447703No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG11440.10101081451447703No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11410.10074592601487613No Hit
CATCAAGGTTCAGCGGCAGTGGATTTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG11380.10048103751527525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)