FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100038
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTACACTACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT49810.4528025395486338No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47100.42816702695725056No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45120.4101676487539521No Hit
GTCTAAAGTGGATGCACCATAGATCAGGAGCTTGGGGGCCTTCCCGGGTTTTTGCTGATACCAAGTTAAATAC44080.40071342989969433No Hit
ACACTACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA43950.3995316525429122No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38350.34862432025075496No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAACT38260.34780616669605957No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAACTCTGTC34860.31689814351867845No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33240.30217137953416157No Hit
ACTTTAGACAGTGGAGTCCCATCAAGGTTGAGTGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCAGCAGT32540.2958079629976419No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31750.2886263929064269No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30430.27662680743756124No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27850.25317307220296026No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26760.24326432359609396No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26720.24290069979400714No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26250.23862812011948678No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACC26180.23799177846583483No Hit
GATCTATGGTGCATCCACTTTAGACAGTGGAGTCCCATCAAGGTTGAGTGGCAGTGGATCTGGGACAGAGTTCA25580.23253742143453227No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25220.22926480721575074No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT24880.22617400489801262No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24740.22490132159070866No Hit
CCATAGATCAGGAGCTTGGGGGCCTTCCCGGGTTTTTGCTGATACCAAGT23840.21671978604375486No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23760.21599253843958116No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTAACAGGTATTTAACTTGGTATCAGCAAAAA22890.20808372074419246No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21870.19881131379097813No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21730.19753863048367423No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21370.19426601626489265No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21140.19217517940289336No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20810.18917528303567693No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20780.18890256518411183No Hit
CTGCTACTCTGGCTCCGAGGTTCCAGATGTGACATCCAAATGACCCAGTC20780.18890256518411183No Hit
GTGGAGTCCCATCAAGGTTGAGTGGCAGTGGATCTGGGACAGAGTTCACT20770.1888116592335901No Hit
ACCTTGATGGGACTCCACTGTCTAAAGTGGATGCACCATAGATCAGGAGCTTGGGGGCCTTCCCGGGTTTTTG20650.1877207878273296No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC20530.1866299164210691No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20130.18299367840020073No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTCAACCTTGATGGGACTCCA19010.1728122119417693No Hit
GTTAAATACCTGTTAATGTCCTGACTTGCCCGGCAAGTGATGGTGACTCT18400.16726694895994504No Hit
CATCAAGGTTGAGTGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTG18160.165085206147424No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18030.1639034287906418No Hit
GTATTTAACTTGGTATCAGCAAAAACCCGGGAAGGCCCCCAAGCTCCTGA17310.15735820035307874No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA17240.15672185869942673No Hit
AGTCAGGACATTAACAGGTATTTAACTTGGTATCAGCAAAAACCCGGGAA17220.15654004679838332No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16850.15317652662908007No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTTCCAGATGTGACAT16420.1492675707566466No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16340.14854032315247293No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16260.14781307554829923No Hit
GTCTCCTACAGATGCAGACAGGGACGAAGGAGACTGGGTCATTTGGATGT16110.1464494862904736No Hit
GGACTCCACTGTCTAAAGTGGATGCACCATAGATCAGGAGCTTGGGGGCC15860.14417683752743088No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15790.1435404958737789No Hit
ACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC15620.14199509471490984No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15430.14026788165499737No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15310.13917701024873688No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14730.13390446511847773No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14690.13354084131639088No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATT14550.13226815800908698No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14420.13108638065230474No Hit
GTCCCAGATCCACTGCCACTCAACCTTGATGGGACTCCACTGTCTAAAGT14130.12845010808717516No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13900.12635927122517587No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13640.12399571651161143No Hit
CCTTCGTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG13580.12345028080848117No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13450.12226850345169893No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTTCCAGATGTGACATCCAAATGACCCAGTCTCCTTCGTCC13280.12072310229282988No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13270.12063219634230818No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13260.12054129039178647No Hit
GTAGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCACT13210.12008676063917792No Hit
GTCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAG12930.11754139402457006No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12860.11690505237091808No Hit
GTTAATGTCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAG12800.11635961666778784No Hit
GATCAGGAGCTTGGGGGCCTTCCCGGGTTTTTGCTGATACCAAGTTAAAT12470.11335972030057143No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12400.11272337864691948No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12370.11245066079535433No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12360.11235975484483263No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG12320.11199613104274579No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12200.11090525963648529No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12120.11017801203231162No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12120.11017801203231162No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12110.11008710608178991No Hit
CACTTTAGACAGTGGAGTCCCATCAAGGTTGAGTGGCAGTGGATCTGGGA11960.10872351682396426No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11810.10735992756613863No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11750.10681449186300837No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11730.10663267996196496No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11670.10608724425883469No Hit
CTCCTGATCTATGGTGCATCCACTTTAGACAGTGGAGTCCCATCAAGGTT11550.10499637285257418No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11460.10417821929787881No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11390.10354187764422683No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11190.10172375863379265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)