FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100038
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52090.47352909626758344No Hit
GAGTTACACTACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT48810.44371194449646284No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46430.422076328272296No Hit
GTCTAAAGTGGATGCACCATAGATCAGGAGCTTGGGGGCCTTCCCGGGTTTTTGCTGATACCAAGTTAAATAC42990.390804681292828No Hit
ACACTACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA38550.3504424392611892No Hit
GGGTAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAACT37830.34389721082362606No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37480.34071550255536626No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36680.33344302651362956No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAACTCTGTC34850.31680723756815676No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33660.3059894294560733No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29490.2680816480885206No Hit
ACTTTAGACAGTGGAGTCCCATCAAGGTTGAGTGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCAGCAGT28980.2634454446119134No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27200.24726418541904915No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27180.24708237351800574No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26640.24217345218983344No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26440.24035533317939925No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26340.23944627367418217No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACACTACC26010.23644637730696577No Hit
GATCTATGGTGCATCCACTTTAGACAGTGGAGTCCCATCAAGGTTGAGTGGCAGTGGATCTGGGACAGAGTTCA25310.23008296077044613No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23200.21090180521036545No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22480.20435657677280236No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTAACAGGTATTTAACTTGGTATCAGCAAAAA21980.19981127924671693No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21880.19890221974149985No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21740.19762953643419592No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21740.19762953643419592No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21490.19535688767115317No Hit
CCATAGATCAGGAGCTTGGGGGCCTTCCCGGGTTTTTGCTGATACCAAGT21450.19499326386906635No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC20820.18926618898619865No Hit
GTGGAGTCCCATCAAGGTTGAGTGGCAGTGGATCTGGGACAGAGTTCACT20500.18635719856950395No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20440.18581176286637371No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20190.18353911410333099No Hit
CTGCTACTCTGGCTCCGAGGTTCCAGATGTGACATCCAAATGACCCAGTC19960.18144827724133167No Hit
ACCTTGATGGGACTCCACTGTCTAAAGTGGATGCACCATAGATCAGGAGCTTGGGGGCCTTCCCGGGTTTTTG19480.17708479161628962No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19380.17617573211107254No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTCAACCTTGATGGGACTCCA19080.17344855359542125No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18840.17126681078290024No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18590.16899416201985748No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18260.16599426565264108No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17170.15608551704577478No Hit
CATCAAGGTTGAGTGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTG16950.15408558613429718No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16910.15372196233221033No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16850.15317652662908007No Hit
GTATTTAACTTGGTATCAGCAAAAACCCGGGAAGGCCCCCAAGCTCCTGA16790.15263109092594984No Hit
GTCTCCTACAGATGCAGACAGGGACGAAGGAGACTGGGTCATTTGGATGT16260.14781307554829923No Hit
AGTCAGGACATTAACAGGTATTTAACTTGGTATCAGCAAAAACCCGGGAA16230.14754035769673413No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTTCCAGATGTGACAT16210.1473585457956907No Hit
GTTAAATACCTGTTAATGTCCTGACTTGCCCGGCAAGTGATGGTGACTCT16180.14708582794412559No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16050.14590405058734335No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15560.14144965901177958No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15290.13899519834769344No Hit
ACTTACTACTGTCAACAGAGTTACACTACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA15260.13872248049612831No Hit
GTCCCAGATCCACTGCCACTCAACCTTGATGGGACTCCACTGTCTAAAGT15030.136631643634129No Hit
GGACTCCACTGTCTAAAGTGGATGCACCATAGATCAGGAGCTTGGGGGCC15020.13654073768360728No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14790.134449900821608No Hit
ACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC14490.1317227223059567No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14490.1317227223059567No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATT14350.1304500389986528No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14020.12745014263143636No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13990.12717742477987123No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13890.12626836527465413No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13830.1257229295715239No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13780.12526839981891533No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTTCCAGATGTGACATCCAAATGACCCAGTCTCCTTCGTCC13420.12199578560013383No Hit
CCTTCGTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG13260.12054129039178647No Hit
GTCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAG13210.12008676063917792No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13180.11981404278761279No Hit
GTAGTAAGTTGCAAAATCTTCAGGCTGCAGACTGCTGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCACT13120.11926860708448253No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGG13000.11817773567822203No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12830.11663233451935297No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12520.11381425005317998No Hit
CTCCTGATCTATGGTGCATCCACTTTAGACAGTGGAGTCCCATCAAGGTT12450.11317790839952802No Hit
GATCAGGAGCTTGGGGGCCTTCCCGGGTTTTTGCTGATACCAAGTTAAAT12340.11217794294378922No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12010.10917804657657282No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11930.10845079897239913No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11770.10699630376405178No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11670.10608724425883469No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11650.10590543235779128No Hit
GTTAATGTCCTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAG11640.10581452640726957No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11300.10272372408953147No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)