FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179869
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38230.3240190224507975No Hit
CCTCTATGGTGCATCCAACAGGGCCACTGGCATCCCCGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA33590.284692622655566No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32120.27223361237561117No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28240.2393486056502883No Hit
GTATCACTGTCAGCAGTTCCATATCTCACCTCAGACGTTCGGCCAAGGGA27240.23087308845304014No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26660.2259572884786362No Hit
CCATAGAGGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGTTAAGTAGTTGCTGCTAACACTCTGA24740.20968429545991973No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23580.19985269551111182No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23220.1968015093201025No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATCACTGTCAGCAGTT23210.19671675414813No Hit
CAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22630.19180095417372608No Hit
GTGTATCACTGTCAGCAGTTCCATATCTCACCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAA22340.1893430541865241No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAACCTGGTACC21300.180528516301386No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21180.17951145423771622No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21150.17925718872179877No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCGGGGATGCCAGTGGCCCTGTT20370.1726462853079452No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG19710.16705244395776142No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19480.16510307500239432No Hit
GTGATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19160.1623909094992749No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19120.162051888811385No Hit
GTCTCTGGTGGCTCCATCAGCGCTAATTATTACTACTACAACTGGGTCCG19050.1614586026075776No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18620.15781413021276092No Hit
GTTCCATATCTCACCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT18380.15578000608542134No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17830.15111847162693487No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17100.1449313440729437No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17020.14425330269716385No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16840.14272770960165915No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC16420.13916799237881494No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16040.13594729584386062No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15370.13026869932170435No Hit
ATATGGAACTGCTGACAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC15340.1300144338057869No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15310.12976016828986947No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15180.1286583510542272No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATCACTGTCAGCAGTTCCATATCTCA14640.12408157176771319No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13790.11687738215005225No Hit
GTCAGAGTGTTAGCAGCAACTACTTAACCTGGTACCAGCAGAAACCTGGC13770.11670787180610728No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCGGGGATGCCA13420.11374144078707042No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13390.11348717527115298No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13380.1134024200991805No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13320.11289388906734561No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13060.11069025459606108No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC12980.11001221322028124No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12890.1092494166725289No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12810.10857137529674905No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12670.1073848028891343No Hit
CTACTTAACCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCCTCTATGGTGCATCCAACAGGGCCA12640.10713053737321686No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12250.10382508566629006No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12220.10357082015037263No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12160.10306228911853774No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11840.10035012361541831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA1750.052.33721527
TCGTATG1700.050.62521440
CGCGTAG1900.050.0289528
ATGCCGT1750.049.4858744
CGTATGC1850.048.45436541
GCGTAGT2050.046.39787329
GACGCGT2000.045.76591526
ACGAGAC2100.043.53854422
AGACGCG2200.041.58948525
CGAGACG2200.041.57185423
CGTAGTA2300.041.3951130
GGTATCA10500.040.6290861
GTCGTAT350.001373873639.27532239
CTTATAC2550.038.5046841
GTATGCC2350.038.20098542
CACGAGA2400.038.09299521
GAGACGC2500.036.58633424
ATCTCGT2100.035.88966837
CCCACGA2600.035.15978219
CGGCCTA400.002399279335.0597150
Produced by FastQC (version 0.11.5)